Microsatellite loci polymorphism of chloroplast DNA of scots pine (Pinus sylvestris L.) in Asia and eastern Europe

Семериков, В. Л.; Семерикова, С. А.; Dymshakova, O. S.; Зацепина, К. Г.; Tarakanov, V. V.; Тихонова, И. В.; Экарт, А. К.; Vidyakin, A. I.; Jamiyansuren, S.; Rogovtsev, R. V.; Kalchenko, L. I.

doi:10.1134/s1022795414040127

Cited by 27 publications

(15 citation statements)

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“…By comparison, an excess of private alleles was observed in the peripheral Scots pine populations from Spain and Italy based on an analysis of the allozyme and nSSR loci (Prus-Głowacki et al 2006;Belletti et al 2012), as well as in those from Scotland, and the Iberian and Anatolian Peninsulas based on the nSSR loci . Our findings indicated a higher haplotype diversity (H H = 0.984-1.000) and genetic distance between individuals in the populations (D 2 sh = 6.7-11.16) than was found in the previous studies on Scots pine populations representing a wide distribution range of the Scots pine in Europe and Asia (Semerikov et al 2014: H H = 0.924-0.992, D 2 sh = 2.5-6.0; Wójkiewicz and Wachowiak 2016: H H = 0.98-1.00, D 2 sh = 4.15-11.24). Whereas the long distance gene flow occurring by pollen migration tends to have a homogenizing effect on the genetic variation of Scots pine populations, our results revealed a very high and significant differentiation between all the analyzed populations, as well as shmean genetic distance among individuals in population (for abbreviations see Table 1) between the three analyzed regions (PhiPT = 8%).…”

Section: Discussioncontrasting

confidence: 54%

“…Previous research on the variation of native populations of P. sylvestris across its broad European distribution using cpSSR markers revealed a much lower inter-population genetic differentiation: from 0.0 to 8% . Similarly low values of the PhiPT (0.41-1.7%) were found by Semerikov et al (2014) between 38 Scots pine populations in Asia and Eastern Europe. The presented findings indicate contrasting trends in genetic differentiation among the populations within various geographical regions.…”

Section: Discussionmentioning

confidence: 52%

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Genetic resources of relict populations of Pinus sylvestris (L.) in Western Carpathians assessed by chloroplast microsatellites

et al. 2019

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The relict character of the Scots pine populations from the Tatra Mts. (Western Carpathians) was formed on scarcely accessible, ecologically extreme habitats, in areas which were not under human activity. An exhaustive genetic analysis of the Scots pine populations from the refugial locations in the Tatra Mts. (Poland) had not yet been. In this study, we characterize the genetic variation and differentiation of the relict Scots pine populations from the Tatra Mts., to provide information on their genetic resources and the conservation implications. Eight paternally inherited chloroplast microsatellite loci were used to investigate the genetic structure of 4 relict populations, which were compared with the natural populations from the Baltic region (5 populations from Lithuania, Latvia and Estonia) and to a northern range from Russia (5 populations from the Kola Peninsula and Arkhangelsk Oblast). The four relict pine populations were characterized by a lower level of genetic variation when compared to the Baltic and northern populations, which was expressed in lower values of particular genetic parameters: numbers of alleles and haplotypes, haplotype genetic diversity, haplotype differentiation, and mean genetic distance between individuals in the population. Our results revealed a very high and significant genetic differentiation between all the analyzed populations, as well as between the three analyzed regions (PhiPT = 8%). Furthermore, the relict populations within a close geographic location showed higher differentiation (PhiPT = 5%) compared to the northern Russian and Baltic populations that were separated by tens and hundreds of kilometers. The relict populations of P. sylvestris from the Tatra Mts. deserve conservation efforts due to the fact that they are an important component if the species-poor Tatra forests.

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Section: Discussioncontrasting

confidence: 54%

Section: Discussionmentioning

confidence: 52%

Genetic resources of relict populations of Pinus sylvestris (L.) in Western Carpathians assessed by chloroplast microsatellites

et al. 2019

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“…(). On the other hand, the contribution to the European part of the species' range the system of eastern refugia located in Western Siberia, Central Siberia, Altai (Western Mongolia) or Yakutia proposed by some authors (Sannikov & Petrova, ; Sannikov, Petrova, Egorov, & Sannikova, ; Semerikov et al., ) seems rather limited. Definitely, wider sampling in east European and Asiatic parts of the range is needed for a more precise reconstruction of post‐glacial migration routes of Scots pine in that region.…”

Section: Discussionmentioning

confidence: 97%

Tertiary remnants and Holocene colonizers: Genetic structure and phylogeography of Scots pine reveal higher genetic diversity in young boreal than in relict Mediterranean populations and a dual colonization of Fennoscandia

Dering

Kosiński

Wyka

et al. 2017

Diversity and Distributions

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Aim: Scots pine is one of the dominant tree species in forest ecosystems of the temperate and boreal zones in Eurasia. Since the Tertiary, it has persisted also in the Mediterranean region, forming relict populations. In this study, we investigate rangewide genetic diversity of Scots pine, aiming to provide comprehensive information on the phylogeography and genetic resources of the species.Location: Europe and Asia. Methods: Thirteen paternally inherited chloroplast microsatellite (cpSSR) loci were used to investigate the genetic structure of 62 populations from Fennoscandia and 30 populations from the Mediterranean. We also surveyed variability of two maternally inherited mitochondrial DNA (mtDNA) regions and additionally included literature data from 156 populations (248 populations in total).Results: Scots pine retains an overall high genetic diversity in the chloroplast genome but, unexpectedly, the historically younger populations from Fennoscandia showed statistically higher mean intrapopulation diversity than the Mediterranean stands (0.807 and 0.750, respectively). The latter also contained a lower number of private cpSSR haplotypes.Population differentiation with respect to mtDNA was higher (G ST = 0.628) than at cpSSR (G ST = 0.015) indicating an efficient pollen-mediated gene flow among refugial populations of the species. One novel haplotype mtDNA was found, increasing the number of known mitotypes for the species from 4 to 5. The geographic distribution of mitotypes was structured into four groups corresponding to the main refugial areas of the species. Main conclusions: Genetic impoverishment of the Mediterranean populations may enhance their vulnerability to future environmental changes. The spatial distribution of two mitotypes in Fennoscandia, featuring predominance of mitotype a in Norway and Sweden and mitotype b in Finland, gives strong support for dual colonization of that region from south-eastern (Finland) and south-western (Sweden) directions. These results thus provide new insights into both Holocene expansion of Scots pine and the present distribution of species' genetic resources. | 541 DERING Et al.

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“…Provan et al [19] observed lower values for the number of haplotypes (from 10 to 18) by analyzing the loci of 17 cpSSRs in populations of the naturally-occurring range of Scots pine in Europe. A very high number of haplotypes (36)(37)(38)(39)(40)(41)(42)(43) was found across three Estonian populations of this species [31]. In total, 152 haplotypes were identified of four cpSSR loci in 38 populations of P. sylvestris in Asia and Eastern Europe [32].…”

Section: Discussionmentioning

confidence: 99%

“…A very high number of haplotypes (36)(37)(38)(39)(40)(41)(42)(43) was found across three Estonian populations of this species [31]. In total, 152 haplotypes were identified of four cpSSR loci in 38 populations of P. sylvestris in Asia and Eastern Europe [32]. The alleles of six cpSSR loci were combined into 32 different haplotypes, all found in the set of 235 individuals from the 12 different populations of P. pinaster [33].…”

Section: Discussionmentioning

confidence: 99%

Genetic Diversity among Age Classes of a Pinus sylvestris (L.) Population from the Białowieża Primeval Forest, Poland

Wojnicka-Półtorak

Celiński

Chudzińska

2017

Forests

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The Białowieża Primeval Forest (BPF) is Europe's last primeval forest and an irreplaceable area for biodiversity conservation due to its size, protection status, and substantially undisturbed nature. There is no other forest in Europe with such a large surface representing highly-advanced natural succession. This article reports on the first analysis of the genetic variability and demographic structure of a self-renewed Pinus sylvestris population located in BPF, using both chloroplast and mitochondrial DNA markers. The analysis of molecular variance (AMOVA) for chloroplast simple sequence repeats (cpSSRs) revealed a significant genetic differentiation among age classes that accounted for about 2% of the total variance, comparable to those reported among different populations of Scots pine. None of the 117 detected chloroplast haplotypes were common to all age classes. Haplotype diversity ranged from 0.370 to 0.415 for cpSSRs and from 0.320 to 0.455 for mitochondrial markers. The genetic variation of the studied age classes-represented by mitochondrial markers-strongly depicts the maternal genetic structure, indicating limited seed dispersal. Temporal genetic substructuring is maintained within a self-renewed population of Scots pine from the BPF.

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Microsatellite loci polymorphism of chloroplast DNA of scots pine (Pinus sylvestris L.) in Asia and eastern Europe

Cited by 27 publications

References 29 publications

Genetic resources of relict populations of Pinus sylvestris (L.) in Western Carpathians assessed by chloroplast microsatellites

Genetic resources of relict populations of Pinus sylvestris (L.) in Western Carpathians assessed by chloroplast microsatellites

Tertiary remnants and Holocene colonizers: Genetic structure and phylogeography of Scots pine reveal higher genetic diversity in young boreal than in relict Mediterranean populations and a dual colonization of Fennoscandia

Genetic Diversity among Age Classes of a Pinus sylvestris (L.) Population from the Białowieża Primeval Forest, Poland

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