Microsatellite Loci Transferability in Chestnut

Wang, Ying; Kang, Ming; Huang, Hongwen

doi:10.21273/jashs.133.5.692

Cited by 13 publications

(7 citation statements)

References 31 publications

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“…Three out of six (50 %) of the microsatellite loci from F. sylvatica were transferred to F. grandifolia and three out of nine (33.3 %) of the F. crenata ones were transferred, in contrast to the 100 % transferability reported between the closely related species F. sylvatica and F. orientalis (PASTORELLI et al, 2003) and F. crenata and F. japonica (TANAKA et al, 1999). These findings support the idea that the proportion of microsatellites that can be transferred between species decreases with increasing genetic distance (STEINKELLNER et al, 1997;WANG, 2008). The allelic richness, or number of alleles per locus, increased in F. grandifolia for FG7, FG11-12, FS1-15 and FS3-4 compared to the number in their respective species of origin.…”

Section: Discussionsupporting

confidence: 67%

“…Because development of microsatellite markers is expensive and time consuming, and heterologous species often share marker loci, SSR primers often are adapted from closely related species. Previous work has shown successful transferability among members of the Fagaceae family within the same genus such as in Quercus (STEINKELLER et al, 1997;ALDRICH et al, 2003) and Castanea (KUBISIAK and ROBERDS, 2006;WANG, 2008). In Fagus, transferability between the closely related species F. crenata and F. japonica (TANAKA et al, 1999) and F. sylvatica and F. orientalis (PASTORELLI et al, 2003) has also been reported.…”

Section: Introductionmentioning

confidence: 94%

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Use of Microsatellite Markers in an American Beech (Fagus grandifolia) Population and Paternity Testing

2010

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Cross-species amplification of six microsatellite markers from European beech (Fagus sylvatica Linn) and nine markers from Japanese beech (Fagus crenata Blume) was tested in American beech (Fagus grandifolia Ehrh.). Three microsatellites from each species were successfully adapted for use in American beech and were found to be highly polymorphic, with 4-22 alleles at each locus and an expected heterozygosity value of 0.291 to 0.913. Twenty-five trees (including two clonal clusters) from a mature stand were sampled and genotyped to compute population statistics. No linkage disequilibrium between pairs of loci was detected, and the marker loci indicated that the population is at Hardy- Weinberg equilibrium. The markers were also used to genotype two full-sibling families consisting of a combined total of 99 individuals and were found to contain sufficient genetic information to assign paternity using a maximum likelihood method.

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Section: Discussionsupporting

confidence: 67%

Section: Introductionmentioning

confidence: 94%

Use of Microsatellite Markers in an American Beech (Fagus grandifolia) Population and Paternity Testing

2010

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“…It appears that the forces causing tandem repeats such as mutation, replication slippage etc., occurred more frequently in non-coding regions than the genic regions [ 22 , 45 , 47 ]. It is also possible that the lethal mutations in genic regions would subsequently eliminate the genotype while the sequence variations in non-coding regions of the genome would persist, resulting in the observation of higher frequency of sequence variations in the non-coding genomic regions.…”

Section: Resultsmentioning

confidence: 99%

Development and characterization of microsatellite markers for Morus spp. and assessment of their transferability to other closely related species

et al. 2013

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BackgroundAdoption of genomics based breeding has emerged as a promising approach for achieving comprehensive crop improvement. Such an approach is more relevant in the case of perennial species like mulberry. However, unavailability of genomic resources of co-dominant marker systems has been the major constraint for adopting molecular breeding to achieve genetic enhancement of Mulberry. The goal of this study was to develop and characterize a large number of locus specific genic and genomic SSR markers which can be effectively used for molecular characterization of mulberry species/genotypes.ResultWe analyzed a total of 3485 DNA sequences including genomic and expressed sequences (ESTs) of mulberry (Morus alba L.) genome. We identified 358 sequences to develop appropriate microsatellite primer pairs representing 222 genomic and 136 EST regions. Primers amplifying locus specific regions of Dudia white (a genotype of Morus alba L), were identified and 137 genomic and 51 genic SSR markers were standardized. A two pronged strategy was adopted to assess the applicability of these SSR markers using mulberry species and genotypes along with a few closely related species belonging to the family Moraceae viz., Ficus, Fig and Jackfruit. While 100% of these markers amplified specific loci on the mulberry genome, 79% were transferable to other related species indicating the robustness of these markers and the potential they hold in analyzing the molecular and genetic diversity among mulberry germplasm as well as other related species. The inherent ability of these markers in detecting heterozygosity combined with a high average polymorphic information content (PIC) of 0.559 ranging between 0.076 and 0.943 clearly demonstrates their potential as genomic resources in diversity analysis. The dissimilarity coefficient determined based on Neighbor joining method, revealed that the markers were successful in segregating the mulberry species, genotypes and other related species into distinct clusters.ConclusionWe report a total of 188 genomic and genic SSR markers in Morus alba L. A large proportion of these markers (164) were polymorphic both among mulberry species and genotypes. A substantial number of these markers (149) were also transferable to other related species like Ficus, Fig and Jackfruit. The extent of polymorphism revealed and the ability to detect heterozygosity among the cross pollinated mulberry species and genotypes render these markers an invaluable genomic resource that can be utilized in assessing molecular diversity as well as in QTL mapping and subsequently mulberry crop improvement through MAS.

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“…To maintain diversity within fine hardwood forests, additional research with respect to management and conservation must be initiated as indicated with C. sativa (Wang et al 2008b), F. sylvatica (Buiteveld et al 2007), F. excelsior (Heuertz et al 2004;Harbourne et al 2005), J. regia (Aradhya et al 2007;McGranahan 2007), and Ulmus spp.…”

Section: Marker Technologies In Conservation and Tree Improvementmentioning

confidence: 99%

“…American chestnut has been transformed to express the wheat germin-like oxalate oxidase gene in an effort to bolster its resistance to fungal pathogens (Welch et al 2007). With hardwood genetic modification, Gao and Jiang 2009;Howland et al 1995;Jiang et al 2007;Kulju et al 2004;Schenk et al 2008;Truong et al 2007;Wang et al 2008a;Wang et al 2008b Carya AFLP, isozymes, microsatellites, SSR Beedanagari and Conner 2004;Beedanagari et al 2005;Grauke et al 2003;Rϋter et al 1999;2000;Vendrame et al 2000 Castanea AFLP, isozymes, ISSRs, microsatellites, QTL, RFLP, RAPD, SSR Barakat et al 2009;Batista et al 2008;Buck et al 2003;Casasoli et al 2004;Casasoli et al 2006;Clark et al 2001;Feng et al 2007;Marinoni et al 2003;Sisco et al 2005Sisco et al , 2009Tanaka et al 2005; RAPD Buiteveld et al 2007;Jump and Peñuelas 2007;Kraj and Sztorc 2009;Sander et al 2000;Scalfi et al 2004;Scalfi et al 2005;Sisco et al 2009;Vornam et al 2004 Fraxinus Microsatellites, RAPD, RFLP Bacles and Ennos 2008;Harbourne et al 2005;Heuertz et al 2003;Heuertz et al 2004;Lefort et ...…”

Section: Genomic Tools and Genetic Engineering In Hardwood Tree Impromentioning

confidence: 99%

Biotechnological efforts for preserving and enhancing temperate hardwood tree biodiversity, health, and productivity

Pijut

Lawson

Michler

2010

In Vitro Cell.Dev.Biol.-Plant

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Hardwood tree species in forest, plantation, and urban environments (temperate regions of the world) are important biological resources that play a significant role in the economy and the ecology of terrestrial ecosystems, and they have aesthetic and spiritual value. Because of these many values of hardwood tree species, preserving forest tree biodiversity through the use of biotechnological approaches should be an integral component in any forestry program in addition to large-scale ecologically sustainable forest management and preservation of the urban forest environment. Biotechnological tools are available for conserving tree species as well as genetic characterization that will be needed for deployment of germplasm through restoration activities. This review concentrates on the biotechnological tools available for conserving, characterizing, evaluating, and enhancing hardwood forest tree biodiversity. We focus mainly on species grown for lumber and wood products, not species grown mainly for fiber (pulp and paper production). We also present a brief summary of the importance of non-wood forest products from temperate hardwood tree species (a research area that needs further development using biotechnological techniques) and a few case studies for preserving forest tree biodiversity.

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Microsatellite Loci Transferability in Chestnut

Cited by 13 publications

References 31 publications

Use of Microsatellite Markers in an American Beech (Fagus grandifolia) Population and Paternity Testing

Use of Microsatellite Markers in an American Beech (Fagus grandifolia) Population and Paternity Testing

Development and characterization of microsatellite markers for Morus spp. and assessment of their transferability to other closely related species

Biotechnological efforts for preserving and enhancing temperate hardwood tree biodiversity, health, and productivity

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