Microsatellite marker based analysis of genetic diversity in short day tropical Indian onion and cross amplification in related Allium spp.

Khar, Anil; Lawande, K. E.; Negi, K. S.

doi:10.1007/s10722-010-9616-y

Cited by 46 publications

(60 citation statements)

References 35 publications

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“…The ACM238 produced the highest number of bands (14). The EST-SSRs markers showed a higher percentage of amplification than in previous reports (Jakše et al 2005;Khar et al 2011;Mallor et al 2014;Mitrová et al 2015). The bands of ACM315 (290 bp), ACM238 (153 bp) and ACM300 (184 bp, 207 bp, 216 bp) were excluded from further evaluation because of being monomorphic across the studied set of 96 accessions.…”

Section: Resultsmentioning

confidence: 72%

Molecular characterization of Turkish onion germplasm using SSR markers

Hancı¹,

Gökçe²

2016

Czech J. Genet. Plant Breed.

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Hanci F., Gökçe A.F. (2016): Molecular characterization of Turkish onion germplasm using SSR markers. Czech J. Genet. Plant Breed., 52: 71-76.In this study, molecular markers were used for the first time to study the genetic diversity of Turkish onion (Allium cepa L.) accessions. We used 83 turkish landraces, 3 breeding lines and 10 commercial varieties, in total 96 accessions, to study the genetic diversity. Bulked samples of 10 seeds were used to generate a representative profile of the accessions. Variability was evaluated at 46 microsatellite loci. We identified 308 alleles with these markers, out of which 303 were polymorphic. A dendrogram based on the UPGMA analysis grouped the 96 accessions into five main clusters. Dice's similarity coefficient ranged from 0.407 to 0.767 with an average of 0.587. The results showed that 44 out of the 46 SSR markers were convenient and polymorphic enough to distinguish all the studied accessions.

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Section: Resultsmentioning

confidence: 72%

Molecular characterization of Turkish onion germplasm using SSR markers

Hancı¹,

Gökçe²

2016

Czech J. Genet. Plant Breed.

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“…Jakse et al (2005) identified 398 SNP, indels and SSRs which distinguished 35 elite onion populations. The diversity assessment of tropical Indian onion and cross amplification of genomic and expressed sequence tag (EST)-SSR markers in distantly related native wild species were estimated (Khar et al 2010) (Table 1).…”

Section: Diversity Analysis and Varietal Identification Of Alliumsmentioning

confidence: 99%

“…of onion diversity has not been fully exploited but with the liberalisation of policies multinational companies have entered in hybrid onion development (Khar et al 2010). Here we have reviewed the use of molecular markers for diversity analysis, varietal identification, colour and quality improvement, male sterility analysis and recent efforts of genome sequencing in Alliums.…”

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confidence: 99%

Molecular markers in the improvement of Allium crops

Chinnappareddy¹,

Khandagale²,

Chennareddy³

et al. 2013

Czech J. Genet. Plant Breed.

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Chinnappareddy L.R.D., Khandagale K., Chennareddy A., Ramappa V.G. (2013) The genus Allium (Family: Alliaceae) is the most important among the bulbous vegetable crops. Characterization of Alliums based on phenotypic traits is influenced by the environment and leads to biased diversity estimates. Recognizing the potential of DNA markers in plant breeding, researchers have adopted the molecular markers for marker-assisted selection (MAS), quantitative trait loci (QTL) mapping and characterization of different quality traits in Alliums. This review presents details about the use of DNA markers in Alliums for cultivar identification, diversity studies, SSR development, colour improvement, total soluble solids (TSS), cytoplasmic male sterility (CMS) and efforts of DNA sequencing. As there are no such reports to describe the above work under a single heading, we decided to mine literature for those who are working in onion, garlic, chives and leek improvement to generate new insights in the subject.

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“…Other widely used markers like AFLP (Volk et al, 2004;García-Lampasona et al, 2012) and SSR (Fischer and Bachmann, 1998;Song et al, 2004;Khar et al, 2011;Lee et al, 2011;Cunha et al, 2012) have been used in the study of Allium. Initially, we also tried some SSR primers with our investigated species.…”

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confidence: 99%

RAPD and ISSR analysis of some economically important species, varieties and cultivars of the genus Allium (Alliaceae)

Mukherjee¹

2013

Turk J Bot

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IntroductionAllium L. is one of the most economically important genera of plants. Onion (Allium cepa L.), garlic (A. sativum L.), and leek (A. porrum L.) are among the major crops of the world. Besides these, there are some minor Allium crops like A. tuberosum Rottl. and A. stracheyi Baker. Many Allium species are used as foods, spices, and herbal remedies in widespread areas of the world (Krest et al., 2000). These crops possess a wide range of bioactive compounds, especially volatile sulphur compounds (Krest et al., 2000), which help to reduce blood lipids and cholesterol as well as platelet activity, which ultimately lowers the risk of cardiovascular diseases (Sainani et al., 1976;Kendler 1987;Augusti, 1990).Allium is a very large genus with about 750 species (Stearn, 1992). Classification of such a large genus proved to be difficult (Hanelt, 1990). Many ambiguities remain in the phylogeny of Allium (Fritsch & Friesen, 2002). Additionally, there is great morphological diversity at the intraspecific level in Allium cepa, A. sativum, and A. porrum. These variations should be measured at the molecular level for their proper characterisation, which ultimately will be beneficial for future breeding programmes.DNA-based molecular markers have been used previously in studies of genetic diversity and phylogenetic analysis in plants (Savolainen & Chase, 2003;Nybom, 2004). Among the different molecular markers, RAPD has been widely used for the assessment of genomic variation and genetic diversity studies in plants like Lathyrus L.

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Microsatellite marker based analysis of genetic diversity in short day tropical Indian onion and cross amplification in related Allium spp.

Cited by 46 publications

References 35 publications

Molecular characterization of Turkish onion germplasm using SSR markers

Molecular characterization of Turkish onion germplasm using SSR markers

Molecular markers in the improvement of Allium crops

RAPD and ISSR analysis of some economically important species, varieties and cultivars of the genus Allium (Alliaceae)

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