Microsatellite marker based diversity analysis and DNA fingerprinting of Asiatic cotton (Gossypium arboreum) varieties of India

Santosh, H. B.; Meshram, Mithila; Santhy, V.; Waghmare, V. N.

doi:10.1007/s13562-021-00675-z

Cited by 9 publications

(11 citation statements)

References 19 publications

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“…Pattanaik et al (2018) carried out the comparison of traditional growout test and DNA-based PCR assay to estimate F 1 hybrid purity in cauli ower and proposed that molecular marker-based hybrid purity assessment may serve as an effective substitute to traditional GOT. A combination of SSR markers and morphological descriptors are proposed for comprehensive and unambiguous cultivar identi cation and differentiation (Santhy et al 2019;Santosh et al 2020). The present study has identi ed polymorphic SSR markers which can be used in hybrid purity testing.…”

Section: Con Rmation Of Genetic Puritymentioning

confidence: 99%

“…as an effective substitute to traditional GOT. A combination of SSR markers and morphological descriptors are proposed for comprehensive and unambiguous cultivar identi cation and differentiation (Santhy et al 2019;Santosh et al 2020). The present study has identi ed polymorphic SSR markers which can be used in hybrid purity testing.…”

Section: Con Rmation Of Genetic Puritymentioning

confidence: 99%

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WITHDRAWN: Microsatellite Marker Based DNA Fingerprinting of Cotton (Gossypium Spp.) Hybrids and Their Parents

Bargat

Venoor

et al. 2021

Preprint

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Cotton production in India by vast majority comes from cotton hybrids whose genetic purity is of great significance in seed production chain and trade. Therefore, there is need to develop a rapid, reliable and reproducible technique to assess the genetic purity of cotton hybrids as traditional, morphological traits-based ‘Grow-Out Test’ is resource intensive, time consuming, tedious and not an infallible procedure. In this regard, a study was planned to understand the genetic diversity among the hybrids and their parents, and also to identify SSR markers for confirmation of genetic purity or hybridity. One intra-arboreum hybrid, CICR2 (DS 5 GMS × LD 327 Sel.), four intra-hirsutum hybrids viz., CSHH198 (CSH 19 × CSH 8), CSHH238 (SH 2379 9Y × PIL 8 Sel.), CSHH243 (CSH 2013 × CSH 43), CSHH1862 (GMS 16A × CB 33) and one hirsutum × barbadense hybrid, Phule 388 (RHC-006 × RHCb-001) along with their respective parental lines were selected for molecular characterization. Of the total 215 SSR markers surveyed, 60 markers conveyed polymorphism. The information conveyed by the polymorphic SSR markers was utilized to assess the molecular divergence among the study material. Maximum genetic dissimilarity of 0.66 was noted between Phule 388 and LD 327 (Sel.), and between RHC-006 and DS 5 (GMS). Minimum genetic dissimilarity of 0.07 was observed between CSHH1862 with CB 33, followed by 0.11 between CICR2 with DS 5 (GMS). SSR markers were highly efficient in capturing both intra-species and inter-species level diversity. The clustering and factorial analysis was in congruence with the species of Gossypium. The diploid species genotypes were clustered separately and distinctly from the rest of the genotypes. All the hirsutum hybrids and their respective parents were found closely clustered. The inter-specific hybrid, Phule 388 along with its parents was found grouped closely. The genetic purity of the hybrids was confirmed using identified SSR markers [GH486, BNL1421, BNL3594, JESPR151 for G. hirsutum hybrid CSHH198; GH486, BNL2449, JESPR151, TMB0436 for G. hirsutum hybrid CSHH238; BNL2449, JESPR151, JESPR152 for G. hirsutum hybrid CSHH243; and, GH527, BNL3812, TMB1484, TMB1645, NAU1190, BNL3816 for inter-specific G. hirsutum × G. barbadense hybrid Phule 388]. The SSR markers were efficient in analysis of hybrid seed purity. The information generated in the present study about genetic diversity and genetic purity testing will greatly facilitate quality seed production of these cotton hybrids and thus, better cotton production.

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Section: Con Rmation Of Genetic Puritymentioning

confidence: 99%

Section: Con Rmation Of Genetic Puritymentioning

confidence: 99%

WITHDRAWN: Microsatellite Marker Based DNA Fingerprinting of Cotton (Gossypium Spp.) Hybrids and Their Parents

Bargat

Venoor

et al. 2021

Preprint

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“…Of these marker types, SSRs have the advantages of high polymorphism, good reproducibility, co-dominance, and simple operation [12,13]. SSRs have been effectively utilized for cotton DNA fingerprinting, genetic diversity analysis, and QTL mapping [8,[14][15][16][17][18], and this has enhanced the protection of cotton germplasm resources and enabled the genetic improvement of cotton varieties in China. DNA fingerprinting through PCR-based molecular markers had been reported in cotton.…”

Section: Introductionmentioning

confidence: 99%

Use of SSR Markers for the Exploration of Genetic Diversity and DNA Finger-Printing in Early-Maturing Upland Cotton (Gossypium hirsutum L.) for Future Breeding Program

et al. 2022

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DNA fingerprinting and genetic diversity analysis of 79 early-maturing upland cotton (Gossypium hirsutum L.) cultivars were performed using Simple Sequence Repeat (SSR) molecular markers. Out of 126 pairs of SSR primers, we selected 71 pairs that gave good polymorphisms and clear bands, had good stability, and showed even distribution on the cotton chromosomes, and 142 polymorphic genotypes were amplified. The average number of alleles amplified with the SSR primers was 2.01. The polymorphism information content (PIC) of the markers ranged from 0.1841 to 0.9043, with an average of 0.6494. The results of fingerprint analysis showed that nine varieties had characteristic bands, and at least six primer pairs could be used to completely distinguish all 79 cotton accessions. Using NTSYS-pc 2.11 cluster analysis, the genetic similarity coefficients between the cotton genotypes ranged from 0.3310 to 0.8705, with an average of 0.5861. All cotton accessions were grouped into five categories at a similarity coefficient of 0.57, which was consistent with the pedigree sources. At the same time, the average genetic similarity coefficients of early-maturing upland cotton varieties in China showed a low-high-low pattern of variation over time, revealing the development history of early-maturing upland cotton varieties from the 1980s to the present. This also indirectly reflects that in recent years, China’s cotton breeders have focused on innovation and have continuously broadened the genetic resources for early-maturing upland cotton.

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“…Of these marker types, SSRs have the advantages of high polymorphism, good reproducibility, co-dominance, and simple operation [12][13] . SSRs have been useded for cotton DNA ngerprinting, genetic diversity analysis, and QTL mapping 8,[14][15][16][17][18] , and this has enhanced the protection of cotton germplasm resources and enabled the genetic improvement of cotton varieties in China. In the current research, DNA ngerprinting, genetic diversity, and the genetic relationships among 79 early-maturing upland cotton accessions were analyzed with SSR markers.…”

Section: Introductionmentioning

confidence: 99%

Analysis of Genetic Diversity And DNA Fingerprinting In Early-Maturing Upland Cotton Using SSR Markers

Kuang

Xiao

Guo

et al. 2022

Preprint

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In this study, DNA fingerprinting and genetic diversity analysis of 79 early-maturing upland cotton (Gossypium hirsutum L.) cultivars were performed using Simple Sequence Repeat (SSR) molecular markers. From 126 pairs of SSR primers, we selected 71 pairs of primers that gave good polymorphisms and clear bands, had good stability, and showed even distribution on the cotton chromosomes, and 142 polymorphic genotypes were amplified. The average number of alleles amplified with the SSR primers was 2.01. The polymorphism information content (PIC) of the markers ranged from 0.1841 to 0.9043 with an average of 0.6494. The results of fingerprint analysis showed that nine varieties had characteristic bands, and at least six primer pairs could be used to completely distinguish all 79 cotton accessions. Using NTSYS-pc 2.11 cluster analysis, the genetic similarity coefficients between the cotton genotypes ranged from 0.3310-0.8705, with an average of 0.5861. All cotton accessions were grouped into five categories at a similarity coefficient of 0.57, which was consistent with the pedigree sources. At the same time, the average genetic similarity coefficients of early-maturing upland cotton varieties in China showed a low-high-low pattern of variation over time, revealing the development history of early-maturing upland cotton varieties from the 1980s to the present. This also indirectly reflects that in recent years, China's cotton breeders have focused on innovation and have continuously broadened the genetic resources for early-maturing upland cotton.

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Microsatellite marker based diversity analysis and DNA fingerprinting of Asiatic cotton (Gossypium arboreum) varieties of India

Cited by 9 publications

References 19 publications

WITHDRAWN: Microsatellite Marker Based DNA Fingerprinting of Cotton (Gossypium Spp.) Hybrids and Their Parents

WITHDRAWN: Microsatellite Marker Based DNA Fingerprinting of Cotton (Gossypium Spp.) Hybrids and Their Parents

Use of SSR Markers for the Exploration of Genetic Diversity and DNA Finger-Printing in Early-Maturing Upland Cotton (Gossypium hirsutum L.) for Future Breeding Program

Analysis of Genetic Diversity And DNA Fingerprinting In Early-Maturing Upland Cotton Using SSR Markers

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