Microsatellite markers and genetic diversity assessment in Lolium temulentum

Kirigwi, Francis M.; Zwonitzer, John C.; Mian, M. A. Rouf; Wang, Zeng‐Yu; Saha, Malay C.

doi:10.1007/s10722-007-9218-5

Cited by 16 publications

(16 citation statements)

References 40 publications

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“…Tissue was sent to DuPont Pioneer and DuPont Crop Genetics for genotyping. The SSR genotyping was performed following procedures described elsewhere (Kirigwi et al, 2008;Schuelke, 2000) and visualized on agarose gel. The SSR marker UMC2000 and indel marker IDP6793 were genotyped at DuPont Crop Genetics via polymerase chain reaction (PCR) and visualization on agarose gel.…”

Section: Disease Evaluationmentioning

confidence: 99%

Yield Effects of Two Southern Leaf Blight Resistance Loci in Maize Hybrids

Santa-Cruz¹,

Kump²,

Arellano³

et al. 2014

Crop Science

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In this study we examined the effects of two quantitative trait loci (QTL) for southern leaf blight (SLB) resistance on several agronomic traits including disease resistance and yield. B73–3B and B73–6A are two near‐isogenic lines (NILs) in the background of the maize (Zea mays L.) inbred B73, each carrying one introgression (called 3B and 6A respectively) encompassing a QTL for SLB resistance. Sets of isohybrid triplets were developed by crossing B73, B73–3B, and B73–6A to several inbred lines. A subset of these triplets for which the B73–3B and/or B73–6A hybrid was significantly more SLB resistant than the B73 check hybrid was selected and assessed in multi‐environment yield trials with and without disease. In the presence of SLB, 3B was associated with an approximately 3% yield increase over B73. 6A was associated with a yield advantage in the presence of SLB in specific pedigrees where the 6A resistance phenotype was highly expressed. Results suggested that both introgressions might confer a yield cost in the absence of SLB, but only introgression 6A was associated with a statistically significant reduction. We present evidence to suggest that the yield cost is associated with the resistance phenotype rather than with linkage drag.

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Section: Disease Evaluationmentioning

confidence: 99%

Yield Effects of Two Southern Leaf Blight Resistance Loci in Maize Hybrids

Santa-Cruz¹,

Kump²,

Arellano³

et al. 2014

Crop Science

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“…The size of the SSR amplification fragments ranged from 126 to 560 bp. The CNL primers developed from conserved sequences of five different cereal species were previously shown to amplify in hexaploid wheat (Triticum aestivum L.), ryegrass (Lolium perenne L.), and tall fescue (Festuca arundinacea Schreb) [22,37,52]. The number of fragments generated and PIC content of the 63 primer pairs are shown in Table 2.…”

Section: Ssr Selection and Amplification In P Virgatummentioning

confidence: 99%

Genetic Diversity in Switchgrass Collections Assessed by EST-SSR Markers

et al. 2008

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Switchgrass (Panicum virgatum L.), is a warm season C 4 perennial grass, native to North American tall grass prairies. The high biomass production potential of switchgrass with low inputs makes it an excellent choice as a sustainable bioenergy crop. The objective of this study was to determine the genetic variability within and among 31 switchgrass populations obtained from Germplasm Resources Information Network (GRIN). Six plants from each population (186 genotypes) were characterized with 24 conserved grass expressed sequence tag-simple sequence repeats (EST-SSR) and 39 switchgrass EST-SSR markers. The partitioning of variance components based on the analysis of molecular variance (AMOVA) revealed that the variability within population was significantly higher (80%) than among populations (20%). Pair-wise genetic distance estimates based on SSR data revealed dissimilarity coefficients for genotypes ranging from 0.45 to 0.81. The uplands and lowlands were generally grouped in distinct sub-clusters. The genotypes were grouped into the different adaptive zones based on the geographical locations of the collections. Ploidy estimation showed that 20 of the accessions were tetraploid, four of them were octoploids and the remaining seven had mixed ploidy levels. Flow cytometry analysis of genotypes within cultivars collected from commercial seed sources did not always support the ploidy mixtures that were found in GRIN collections. Three genotypes of two accessions that clustered differently from other genotypes of the same accessions also had different ploidy levels.

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“…Two major species in the outcrossing group, Italian ryegrass (Lolium multiflorum Lam.) (tall fescue), L. multiflorum and L. perenne can be transferred to L. temulentum (Kirigwi et al 2008;Hirata et al 2011). In contrast, darnel ryegrass (L. temulentum L.) from the inbreeding group is a cosmopolitan weed of cereal crop fields.…”

Section: Introductionmentioning

confidence: 99%

Exceptionally high fertility observed in three F₁ hybrids between Lolium multiflorum Lam. and L. temulentum L.

et al. 2012

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The pollen fertility and seed set of three F1 hybrid plants derived from a cross between a darnel ryegrass (Lolium temulentum L.) line (Ba3081) and a genotype of Italian ryegrass (L. multiflorum Lam.) were evaluated in field and greenhouse experiments under different temperatures. In the field where the daily mean temperature for 3 weeks prior to pollen dispersal was 16.5°C, the mean pollen fertility of the F1 plants was 42.8%, which was lower than the value observed in the parental species (96.0–98.1%). In two greenhouses with a daily mean temperature of 20.4 and 15.8°C, the mean pollen fertility of the F1 plants was 53.1% and 44.0%, respectively, which were higher than the values reported previously for the hybrids between L. temulentum × L. multiflorum (maximum of about 20%). The seed set of the F1 plants was 24% under open‐pollinated conditions in the field compared with 4.2–8.8% under self‐pollinated conditions in the two greenhouses. These results indicated that the F1 plants were self‐fertile and able to set F2 seed, with a significant reduction in pollen fertility occurring with a decrease in the daily mean temperature (2.15% decrease in fertility per unit decrease in temperature). The germination rate of the F2 seeds was approximately 57%, indicating the possibility of producing an F2 population for future genetic analysis. This is the first report of F1 plants from L. temulentum × L. multiflorum with fertile pollen and with the ability to produce progeny by self‐pollination. These findings suggest a possibility of developing recombinant inbred lines between L. temulentum and L. multiflorum.

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Microsatellite markers and genetic diversity assessment in Lolium temulentum

Cited by 16 publications

References 40 publications

Yield Effects of Two Southern Leaf Blight Resistance Loci in Maize Hybrids

Yield Effects of Two Southern Leaf Blight Resistance Loci in Maize Hybrids

Genetic Diversity in Switchgrass Collections Assessed by EST-SSR Markers

Exceptionally high fertility observed in three F₁ hybrids between Lolium multiflorum Lam. and L. temulentum L.

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Microsatellite markers and genetic diversity assessment in Lolium temulentum

Cited by 16 publications

References 40 publications

Yield Effects of Two Southern Leaf Blight Resistance Loci in Maize Hybrids

Yield Effects of Two Southern Leaf Blight Resistance Loci in Maize Hybrids

Genetic Diversity in Switchgrass Collections Assessed by EST-SSR Markers

Exceptionally high fertility observed in three F1 hybrids between Lolium multiflorum Lam. and L. temulentum L.

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Product

Resources

About

Exceptionally high fertility observed in three F₁ hybrids between Lolium multiflorum Lam. and L. temulentum L.