Microsatellite markers associated with body and carcass weights in broiler breeders

Nassar, Farid S.; Moghaieb, Reda E. A.; Abdou, A. M.; Stino, F. K. R.

doi:10.5897/ajb11.3721

Cited by 9 publications

(3 citation statements)

References 43 publications

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“…A total of twelve roosters of Cairo-B2 chicken strain (40 weeks old, 3400 ± 70 g weight, 18th generation) were used for the current study. The Cairo-B2 chicken strain was established in the Animal Production Department (Faculty of Agriculture, Cairo University, Egypt) by crossing between males from Arbor Acers grandparent-female-line with females from White Baladi native Egyptian chicken breed, and selecting the next generation of the parents based on the highest live body weight at 42 d of age [18]. The chickens were reared in cages (50 × 50 × 60 cm) and held in the Agricultural Experiments Station (Cairo University).…”

Section: Animals and Ethical Statementmentioning

confidence: 99%

The In Vitro Evaluation of Rooster Semen Pellets Frozen with Dimethylacetamide

Hamad

Elomda

Sun

et al. 2023

Animals

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Sperm cryopreservation is an effective technique for conserving animal genetic diversity and transmitting superior genetic backgrounds, maintained via a non-invasive sampling and collection of huge quantities of sperm. Nevertheless, cryopreservation in avian species is not commercially viable because of the rooster sperm’s susceptibility to damage. This study aims to estimate the impact of dimethylacetamide (DMA) as a cryoprotectant at different levels (3%, 6%, or 9%) on the post-thawed sperm quality, motility, antioxidant-biomarkers, and the expression of anti-freeze related genes. Semen samples were collected twice a week from twelve roosters aged 40 wk, weighing 3400 ± 70 g, and belonging to the Cairo-B2 chicken strain. Fresh semen samples were rapidly appraised, pooled, diluted with two volumes of a basic extender, and divided equally into three groups. The diluted groups were chilled at −20 °C for 7 min, then gently supplemented with 3, 6, or 9% pre-cooled DMA and equilibrated at 5 °C for a further 10 min. Semen pellets were formed by pipetting drops 7 cm above liquid nitrogen (LN2), which were then kept inside cryovials in the LN2. Thawing was performed 2 months later by taking 3–4 pellets of the frozen semen into a glass tube and warming it in a water bath for 8 s at 60 °C. The results showed that 3% DMA increased the proportion of total motile sperm, progressivity, viability, and plasma membrane integrity (%) compared to the 6% and 9% DMA groups. The lipid peroxidation and antioxidant enzyme activity were improved in the 3% group. At the same time, some anti-freeze-related genes’ (including ras homolog family member A (RHOA), heat shock protein 70 (HSP70), and small nuclear ribonucleoprotein polypeptide A (SNRPA1)) expressions were upregulated within the 3% DMA group relative to other groups. In conclusion, the 3% DMA group maintained higher post-thawed sperm quality than the other tested groups.

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Section: Animals and Ethical Statementmentioning

confidence: 99%

The In Vitro Evaluation of Rooster Semen Pellets Frozen with Dimethylacetamide

Hamad

Elomda

Sun

et al. 2023

Animals

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“…Однак мікросателітні маркери пов'язані також із проявом господарсько-корисних ознак у тварин. У результаті низки досліджень виявлено зв'язок деяких мікросателітних локусів з показниками продуктивності та стійкості до захворювань, що своєю чергою, істотно розширює сферу їхнього застосування (Van Tassell et al, 2000;McElroy et al, 2005;Nassar et al, 2012;Puja et al, 2015). Також є дослідження, насамперед в медицині, в яких виявлено зв'язок мікросателітної нестабільності (високої варіабельності внаслідок особливостей нуклеотидній структури) зі спадковими захворюваннями (так звана мікросателітна експансія) (Kurzawski et al, 2004;Salipante et al, 2014).…”

Section: вступunclassified

Microsatellite diversity in the populations of Ukrainian local chicken breeds

Kulibaba¹,

Liashenko²

2018

SMLNUVMBA

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The article considers the questions about microsatellite diversity in the populations of Birkivska Barvista (line A), White Plymouth Rock (line G-2), Poltava clay (line 14) and Rhode Island Red (line 38) chicken breeds. Using the classical PCR method, populations polymorphism was studied for 14 microsatellite loci (LEI0094, LEI0166, LEI0192, ADL0268, ADL0278, MCW0034, MCW0081, MCW0104, MCW0123, MCW0330, MCW0245, MCW0257, MCW0282, MCW0288). For all microsatellite loci 66 alleles were detected. For the population of White Plymouth Rock chicken breed, the number of individual alleles in all the loci was 64; for Birkivska Barvista – 50; for Rhode Island Red – 50; for Poltava clay – 52. By the values of the polymorphism information content (PIC), the number of highly informative markers was ~ 45% of the total. According to the results of the research, it was revealed that the biggest genetic differences were between the White Plymouth Rock and Rhode Island Red chicken breeds (65.9% of differences), the smallest were between White Plymouth Rock and Poltava clay chicken breeds (32.3%). Between lines 14 and 38 (the egg-meat direction of productivity), 35.9% of the differences were observed. By comparison of the population of Borkovskaya Barvistaya chicken breed (line A), the maximum differences were found with the Rhode Island Red (58.8%), while the G-2 and 14 lines showed similar differences (32.8 and 37.9%). According to Wright's F-statistics analysis, 19.5% of detected genetic variability was between populations that indicating a significant divergence of the experimental chicken lines. Among all studied loci, the average level of divergence (the value of Fst was within the range of 0.06–0.15) is characteristic for 29% of the total number of microsatellite markers; strongly expressed divergence (0.16–0.25) for 57% and very strong (> 0.25) for 14% (locus MCW0257 and MCW0288). By averaged values of Fis, negative values (excess of heterozygotes) were shown only for 3 from all studied loci. The average Fit value indicates a significant (27.5%) excess of homozygous individuals what indicates the high level of inbreeding in experimental chicken populations and reaches its maximum value in the MCW0245 and MCW0257 loci.

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“…A similar trend was also observed in the Cairo B-2 line females (average = 943 g) compared with the random breed control (RBC) line females (average = 718 g). The live body weight of the Cairo B-2 line, at six weeks of age, compared with the RBC line, was significantly higher (45.3% for males and 31.3% for females) due to the selection that had been done for six generations [14][15][16].…”

Section: Introductionmentioning

confidence: 99%

Heritability, Genetic and Phenotypic Correlations in Three Indigenous Egyptian Chicken Lines

Ramadan,

Rashed,

El-Komy

et al. 2024

Egyptian Journal of Veterinary Sciences

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T HE STUDY conducted aimed to examine the heritability, genetic and phenotypic correlations of body weight and egg production traits in Egyptian chickens. These traits are considered crucial in chicken production and the improvement of these traits through crossbreeding and selection programs is important for maximizing production. The data used in this study were obtained from three generations of three different chicken strains: Tanta G-1, Tanta G-2 and Mamourah. The researchers used the MCMC glmm package of R software to estimate the genetic parameters for these economically important traits in Gimmizah Station native chickens. After three generations of selection, the genetic correlations among body weight traits ranged from 0.78 to 0.96, 0.57 to 0.91 and 0.82 to 0.93 for Tanta G-1, Tanta G-2 and Mamourah chickens, respectively. The phenotypic correlation among different body weights ranged from 0.26 to 0.95, 0.17 to 0.96 and 0.38 to 0.97 for Tanta G-1, Tanta G-2 and Mamourah lines, respectively. These correlations increased from one generation to another. For the heritability of body weight traits, they ranged from 0.16 to 0.34, 0.15 to 0.33 and 0.13 to 0.19 for Tanta G-1, Tanta G-2 and Mamourah lines, respectively. In conclusion, the study suggests that simultaneous selection for both growth and egg production traits may result in a reduction in egg number but an increase in egg weight. This finding highlights the need for careful consideration when selecting for these traits to attain a balanced and optimal outcome in chicken production.

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Microsatellite markers associated with body and carcass weights in broiler breeders

Cited by 9 publications

References 43 publications

The In Vitro Evaluation of Rooster Semen Pellets Frozen with Dimethylacetamide

The In Vitro Evaluation of Rooster Semen Pellets Frozen with Dimethylacetamide

Microsatellite diversity in the populations of Ukrainian local chicken breeds

Heritability, Genetic and Phenotypic Correlations in Three Indigenous Egyptian Chicken Lines

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