Microsatellite markers for direct genotyping of the crayfish plague pathogen Aphanomyces astaci (Oomycetes) from infected host tissues

Grandjean, Frédéric; Vrålstad, Trude; Diéguez-Uribeondo, Javier; Jelić, Mišel; Mangombi, Joa Braïthe; Delaunay, Carine; Filipová, Lenka; Rezinciuc, Svetlana; Kozubíková‐Balcarová, Eva; Guyonnet, Daniel; Viljamaa-Dirks, Satu; Petrusek, Adam

doi:10.1016/j.vetmic.2014.02.020

Cited by 53 publications

(84 citation statements)

References 31 publications

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“…This strain is considered highly virulent and has been involved in massive outbreaks, particularly in Norway, Finland, Czech Republic and France (Viljamaa-Dirks et al, 2013;Kozubíková-Balcarova et al, 2014;Grandjean et al, 2014;Strand et al, 2014). This strain is specific to P. leniusculus which often shares the habitat of the white-clawed crayfish in France (Bramard et al, 2006;Filipova et al, 2013) which means that this strain is particularly well adapted to the water conditions of the La Lucelle brook.…”

Section: Discussionmentioning

confidence: 99%

“…As they are invasive, they are now very common in much of Europe . Recently, several molecular methodologies have been developed to detect A. astaci directly from infected crayfish tissues (Oidtmann et al, 2006;Vrålstad et al, 2009Vrålstad et al, , 2014Tuffs and Oidtmann, 2011;Grandjean et al, 2014) and have improved the possibilities for screening for this pathogen (Kozubíková et al, 2008;2011b;Aquiloni et al, 2010;Vrålstad et al, 2011;Pârvulescu et al, 2012). These studies confirmed that all of these North American crayfish species can carry crayfish plague and so can freshwater crab species (Svoboda et al, 2014;Schrimpf et al, 2014).…”

Section: Introductionmentioning

confidence: 91%

“…Eight microsatellite loci developed by Grandjean et al (2014) were genotyped on 5 samples. All of them were positive for A. astaci presence, with level of infection ranging from A4 to A6 (moderate to high infection levels) according to Vrålstad et al (2009).…”

Section: (I) Diagnosis Of Plague Detection and Strain Determinationmentioning

confidence: 99%

“…All of them were positive for A. astaci presence, with level of infection ranging from A4 to A6 (moderate to high infection levels) according to Vrålstad et al (2009). All experimental procedures for microsatellite genotyping were identical to those described in Grandjean et al (2014).…”

Section: (I) Diagnosis Of Plague Detection and Strain Determinationmentioning

confidence: 99%

“…This outbreak gave us the opportunity to follow the spread of mortality front for one year. The A. astaci strain involved in this outbreak was characterized using specific molecular makers recently developed by Grandjean et al (2014). Finally, crayfish from the upstream part, free of plague at the time of translocation, were rescued by French authorities of ONEMA and translocated to a suitable brook free of non-indigenous crayfish.…”

Section: Introductionmentioning

confidence: 99%

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Monitoring of white-clawed crayfish (Austropotamobius pallipes) population during a crayfish plague outbreak followed by rescue

Collas

Becking

Delpy

et al. 2016

Knowl. Manag. Aquat. Ecosyst.

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-A mass mortality was detected in the downstream section of one of the most extensive French populations of the endangered white-clawed crayfish, Austropotamobius pallipes, on June 26, 2013. This population occupied a 12 km stretch of the La Lucelle brook, with an estimated size of around 150 000 individuals. The presence of the crayfish plague pathogen was quickly diagnosed as the cause of the mortality, and monitoring was carried out to follow the spread of the disease from 15 July 2013 for one year. Results showed that after a fast spread between 15 and 25 July 2013 (upstream progression of mortality for about 4 km), the mortality front was limited to a stretch of a few hundred meters from August until December 2013. During winter, mortality was always observed, confirming that disease remained active. In April 2014, the mortality front was halted by a large dam in the brook (2 m high), 0.56 km from brook source. Two months later, 30 live crayfish were observed above the dam. On the 30 August 2014, no crayfish were found above the dam. Infected individuals analysed for microsatellite markers confirmed the Pacifastacus leniusculus strain of Aphanomyces astaci at the origin of this outbreak. Before the crayfish plague spread upstream of the large dam, a sample of 576 individuals was collected from upstream of the dam and translocated to another stream in the same French department. In July 2014, observations by night confirmed the presence of translocated white-clawed crayfish in the receiving brook. Key-words:

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 91%

Section: (I) Diagnosis Of Plague Detection and Strain Determinationmentioning

confidence: 99%

Section: (I) Diagnosis Of Plague Detection and Strain Determinationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Monitoring of white-clawed crayfish (Austropotamobius pallipes) population during a crayfish plague outbreak followed by rescue

Collas

Becking

Delpy

et al. 2016

Knowl. Manag. Aquat. Ecosyst.

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Aphanomyces astaci presence in Japan: a threat to the endemic and endangered crayfish species Cambaroides japonicus?

Mrugała

Kawai

Kozubíková‐Balcarová

et al. 2016

Aquatic Conservation

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1. Spread of the crayfish plague pathogen, Aphanomyces astaci, by North American crayfish species is considered one of the main reasons for substantial declines and local extinctions of native European crayfish populations. Owing to human introductions, several American crayfish species have become established throughout the world, and thus pose a potential threat to indigenous crayfish populations susceptible to crayfish plague.2. In Japan, two such widespread alien species, Procambarus clarkii and Pacifastacus leniusculus, were introduced for aquaculture purposes in the late 1920s and since then successfully expanded their ranges.3. Aggressive interactions with alien crayfish along with habitat modifications have been considered primarily responsible for drastic declines in populations of the Japanese endemic crayfish, Cambaroides japonicus, observed in the last few decades. However, the presence of the crayfish plague pathogen, to which Japanese crayfish are susceptible, may be expected, and could contribute to these declines. 4. Only recently, A. astaci has been reported from Taiwan, and to our knowledge no study focusing on its presence outside of the Western Palearctic has been conducted. 5. To fill this gap, 54 P. clarkii and 47 P. leniusculus individuals from five different Japanese locations were screened using molecular methods recommended by the World Organization for Animal Health. Aphanomyces astaci DNA was detected in all studied populations, altogether in 61% and 21% of examined individuals of P. clarkii and P. leniusculus, respectively. 6. The results provide the first evidence of A. astaci presence in Japan and highlight the threat of pathogen transmission to C. japonicus populations.

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Genetic diversity and parasite facilitated establishment of the invasive signal crayfish (Pacifastacus leniusculus) in Great Britain

Robinson

Leániz

James

et al. 2018

Ecology and Evolution

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Successful establishment of non‐native species is strongly influenced, among other factors, by the genetic variation of founding populations, which can be enhanced by multiple introductions through admixture. Coexisting pathogens can also facilitate the establishment of non‐native species by detrimentally impacting on the native fauna acting as novel weapons. The signal crayfish (Pacifastacus leniusculus) is a highly invasive species, which has caused mass declines of native crayfish in Europe through displacement and transmission of the oomycete Aphanomyces astaci (crayfish plague), which is typically lethal to native European crayfish. However, whether Aphanomyces astaci may have facilitated the invasion of the signal crayfish is not known. We estimated the genetic diversity at microsatellite DNA loci, effective population size, and potential origins of seven infected and noninfected signal crayfish populations in Europe and one founder population in North America. Approximate Bayesian computation analysis and population structuring suggested multiple host introductions from diverse source populations, as well as higher heterozygosity among infected than uninfected populations, which could reflect a fitness advantage. Low effective population size, moderate heterozygosity, and lack of isolation by distance suggest that some invasive signal crayfish populations may not be fully established or that their genetic diversity may have been reduced by eradication attempts.

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Microsatellite markers for direct genotyping of the crayfish plague pathogen Aphanomyces astaci (Oomycetes) from infected host tissues

Cited by 53 publications

References 31 publications

Monitoring of white-clawed crayfish (Austropotamobius pallipes) population during a crayfish plague outbreak followed by rescue

Monitoring of white-clawed crayfish (Austropotamobius pallipes) population during a crayfish plague outbreak followed by rescue

Aphanomyces astaci presence in Japan: a threat to the endemic and endangered crayfish species Cambaroides japonicus?

Genetic diversity and parasite facilitated establishment of the invasive signal crayfish (Pacifastacus leniusculus) in Great Britain

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