Microsatellite Markers for Grapevine: Tools for Cultivar Identification &amp; Pedigree Reconstruction

Sefc, Kristina M.; Pejić, Ivan; Maletić, Edi; Thomas, Mark R.; Lefort, François

doi:10.1007/978-90-481-2305-6_21

Cited by 26 publications

(19 citation statements)

References 88 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…ncbi.nlm.nih.gov/probe). They have been used for many p u r p o s e s , i n c l u d i n g t h e f o l l o w i n g : g e r m p l a s m characterization and pedigree reconstruction (Sefc et al 2009); construction of linkage maps (Cipriani et al 2011); identification and mapping of quantitative trait loci (QTLs) (Welter et al 2011) and marker assisted selection (Töpfer et al 2011). However, to our knowledge, the unique study focused on the extensive design of multiplex PCRs for grapevine genotyping was reported by Merdinoglu et al (2005).…”

Section: Introductionmentioning

confidence: 99%

Whole-genome genotyping of grape using a panel of microsatellite multiplex PCRs

Zarouri¹,

Vargas²,

Gaforio³

et al. 2015

Tree Genetics & Genomes

View full text Add to dashboard Cite

The use of microsatellite markers in large-scale genetic studies is limited by its low throughput and high cost and labor requirements. Here, we provide a panel of 45 multiplex PCRs for fast and cost-efficient genome-wide fluorescencebased microsatellite analysis in grapevine. The developed multiplex PCRs panel (with up to 15-plex) enables the scoring of 270 loci covering all the grapevine genome (9 to 20 loci/chromosome) using only 45 PCRs and sequencer runs. The 45 multiplex PCRs were validated using a diverse grapevine collection of 207 accessions, selected to represent most of the cultivated Vitis vinifera genetic diversity. Particular attention was paid to quality control throughout the whole process (assay replication, null allele detection, ease of scoring). Genetic diversity summary statistics and features of electrophoretic profiles for each studied marker are provided, as are the genotypes of 25 common cultivars that could be used as references in other studies.

show abstract

Section: Introductionmentioning

confidence: 99%

Whole-genome genotyping of grape using a panel of microsatellite multiplex PCRs

Zarouri¹,

Vargas²,

Gaforio³

et al. 2015

Tree Genetics & Genomes

View full text Add to dashboard Cite

show abstract

“…Currently, up to 1,079 V. vinifera SSR probes can be found at the NCBI database (http://www.ncbi.nlm.nih.gov/probe). Microsatellite markers have been used in grape for many purposes, including: germplasm characterization and pedigree reconstruction (Sefc et al, 2009); construction of linkage maps (Cipriani et al, 2011); identification and mapping of QTLs (Welter et al, 2011) and marker assisted selection (Töpfer et al, 2011). …”

Section: Microsatellite Markersmentioning

confidence: 99%

Association study of phenology, yield and quality related traits in table grapes using SSR and SNP markers

Zarouri¹

View full text Add to dashboard Cite

“…To overcome drawbacks of some of these molecular markers using non-specific primers, Simple Sequence Repeats (SSR) is considered as the markers of choice, given to their abundance, high polymorphism, reproducibility, and codominant inheritance (Varshney et al, 2005;Wünsch and Hormaza, 2002;Morgante et al, 1998Morgante et al, , 2002. Advantages of using SSR markers in plant germplasm characterization, in comparison to other PCR based markers have been well proved as reported in high number of published works in many crops (Achtak et al, 2009;Sefc et al, 2009;Zhang et al, 2009;Fossati et al, 2005;Belaj et al, 2003).…”

Section: Introductionmentioning

confidence: 97%