Microsatellite markers in common bean (Phaseolus vulgaris L.)

Abstract

Common bean is one of the most cultivated and consumed grain legumes worldwide, showing a high level of genetic diversity. Here is presented a detailed review of development and mapping of simple sequence repeats (SSRs, microsatellite markers) in the common bean. In the last 25 years, common bean has been the subject of numerous genetic studies, in which the identification and use of SSRs were conducted, and lead to the development of genetic maps. First genetic maps of common bean have been developed in the 1990s and were based on different molecular markers, and included domestication genes and important agronomic traits. Later, SSRs allowed the genetic mapping of more narrow crosses that are often of interest in plant breeding. Most genetic maps have been correlated with the core map established in the recombinant inbred population BAT93 x Jalo EEP558, and includes different markers, RFLP (restriction fragment length polymorphism), RAPD (random amplified polymorphic DNA), AFLP (amplified fragment length polymorphism), and SSRs in particular. More than 2,000 SSR markers are available for the common bean and they are an important tool to evaluate the genetic diversity of common bean landraces. SSRs are also useful to evaluate intra-specific diversity within the genus Phaseolus. Keywords: common bean, genetic diversity, genetic maps, microsatellite markers902 Journal of Central European Agriculture, 2017, 18(4), p.902-917 DOI: 10.5513/JCEA01/18.4.1983 902 Journal of Central European Agriculture, 2017, 18(4), p.902-917 DOI: 10.5513/JCEA01/18.4.1983 902 Journal of Central European Agriculture, 2017, 18(4), p.902-917 DOI: 10. 902Journal of Central European Agriculture, 2017, 18(4), p.902-917 DOI: 10.5513/JCEA01/18.4.1983 Sažetak Grah je jedna od najviše uzgajanih i najviše korištenih zrnatih mahunarki u svijetu koja ima visoku razinu genetske raznolikosti. U ovom radu je prikazan detaljan pregled razvoja i kartiranja mikrosatelitnih biljega (SSR, ponavljajuće jednostavne sekvence) kod graha. U posljednjih 25 godina, grah je bio predmetom brojnih genetskih istraživanja u kojima je provedena identifikacija i upotreba SSR-ova što je dovelo do razvoja genetskih karata. Prve genetske karte graha su razvijene u 1990-ima i bile su bazirane na različitim molekularnim biljezima, a uključivale su gene koji su povezani uz udomaćenje i važna agronomska svojstva. Kasnije su SSR-ovi omogućili genetsko kartiranje križanaca koji su često od interesa u oplemenjivanju bilja. Većina genetskih karata povezana je s osnovnom genetskom kartom uspostavljenom na rekombinantnoj inbred liniji BAT93 x Jalo EEP558, koja uključuje različite biljege kao što su RFLP (polimorfizam dužine restrikcijskih ulomaka), RAPD (nasumično umnožena polimorfna DNA), AFLP (polimorfizam dužine umnoženih ulomaka) te SSR-ove. Više od 2 000 SSR biljega je dostupno za grah i oni su važan alat za procjenu genetske raznolikosti tradicijskih kultivara graha. SSR-ovi su također korisni za procjenu unutarvrsne raznolikosti unutar roda Phaseolus.

ARTIFICIAL HYBRIDIZATION WITHOUT EMASCULATION IN LIMA BEAN (Phaseolus lunatus L.)

“…Other applications of microsatellites in lima bean plants were related to genetic diversity and identification of cross-fertilization rates (PENHA et al, 2017;OLIVEIRA-SILVA et al, 2017). In characterization studies on lima and other bean species of the genus Phaseolus by molecular markers, microsatellites have proven to be effective in tests for kinship determination (VIDAK et al, 2017;GIOIA et al, 2019). Therefore, the use of one or two microsatellite primers with at least one informative band is sufficient to confirm or deny cross-fertilization in lima beans.…”

Characterization and population structure assessment of Indian Common bean using Microsatellite Markers

“…Despite of the diverse classes of currently available markers, knowledge of their genetic informative potential is critical to exploit the germplasm diversity. SSRs are used to examine the genetic diversity and linkage mapping, among other aspects, has been widely explained in the literature (Angioi et al 2008;Vidak et al 2017). For the common bean, the present work is the rst to assess the genetic diversity and structure of common bean from Uttarakhand (India) using SSR markers.…”

Inheritance and identification of ISSR-RGA markers associated with powdery mildew resistance in mungbean for marker-assisted breeding

“…Generally, conventional breeding methods are employed to develop new varieties, however they are limited in low selection efficiency and are subjected to constraints from the environment (Zhao et al, 2020). From the effectiveness of molecular approaches in accelerating breeding for disease resistance, molecular DNA markers which are the powerful genomic tools for revealing differences between individuals of species have been extensively applied to tag genes in many crops (Vidak et al, 2017). Molecular markers that are in close association with the desirable traits can be applicable for selection of the plant materials in a short time at an early stage through marker-assisted selection (MAS), thereby reducing the length of breeding time.…”