Microsatellite polymorphism in Dioscorea tokoro, a wild yam species

Abstract

Six microsatellite loci were characterized in Dioscorea tokoro, a wild yam species in East Asia. All six loci were polymorphic in a sample of 23 individuals from natural populations in Japan. The microsatellite loci displayed many alleles (6.2 alleles per locus on average), and the observed heterozygosity (Ho = 0.54) as well as expected heterozygosity (He = 0.68) were high. The heterozygosities were far more than that previously detected by allozyme analysis of D. tokoro (Ho = 0.23, He = 0.28). Five microsatellite loci were sufficient to provide a paternity exclusion rate (Q) of Q = 0.98, which enables monitoring of the pollen-mediated gene flow between plants in a population. Microsatellite loci are abundant and highly polymorphic in D. tokoro and other plants and are therefore ideal markers for plant population genetic studies.

Assessment of population genetic structure in common wild rice Oryza rufipogon Griff. using microsatellite and allozyme markers

“…Microsatellite loci are a demonstrated rich source of highly polymorphic genetic markers in an ever-increasing number of organisms, and are promising markers for the detection of genetic variation in species, while allozyme analysis has revealed only low levels of variation (Saghai-Maroof et al 1994;Terauchi and Konuma 1994;Lehmann et al 1996;Sanchez et al 1996;Estoup et al 1998;Meglecz et al 1998;Streiff et al 1998;Dje et al 1999;Sun et al 1999;Lemaire et al 2000). For example, in the yam (Dioscorea tokoro), Terauchi and Konuma (1994) found that microsatellites show higher levels of genetic variability than allozymes with respect to heterozygosity. In the current study, all the microsatellite loci surveyed were polymorphic with four to 14 alleles per locus, and an average of 3.1 alleles per locus.…”

“…Since Condit and Hubbell (1991) first noted that microsatellite analysis has great potential for understanding plant population genetics in natural populations, it is now widely used in population and conservation genetic studies (e.g. Bruford and Wayne 1993;Saghai-Maroof et al 1994;Terauchi and Konuma 1994;Chase et al 1996a, b;Inna et al 1997;van Treuren et al 1997;Aldrich et al 1998;Provan et al 1999;Olsen and Schaal 2001). Recently, hundreds of microsatellites from rice have been developed, mapped and published (Wu and Tanksley 1993;Akagi et al 1996;Panaud et al 1996;Chen et al 1997;McCouch et al 1997;Cho et al 2000;Temnykh et al 2000).…”

Assessing Genetic Diversity in Plants with Synthetic Tandem Repetitive DNA Probes

“…Jelinski and Cheliak (1992) (Terauchi and Konuma, 1994) yielding an observed H=0.54 compared to H=0.23 with allozymes. These findings are also consistent with what has been found for animal populations (e.g., see Scribner et al, 1994).…”

Extraction of total DNA and optimization of the RAPD reaction system in Dioscorea opposita Thunb.

“…Molecular markers of DNA can be used to reveal the differences, correlation, and genetic relationships among different varieties of species (Hou et al, 2006). For example, Terauchi and Konuma (1994) analyzed a wild yam species with microsatellite polymorphisms, Mignouna et al (2002) constructed a genetic linkage map of guinea yam and water yam with amplified fragment length polymorphism markers, and Zhou et al (2005) analyzed the genetic diversity of 28 cultivar varieties of D. opposita Thunb. using inter-simple sequence repeat markers.…”