Microsatellite records for volume 7, issue 4

Gélin, Pauline; Rougeux, Clément; Mehn, Vincent; Guillaume, Mireille M. M.; Bruggemann, Henrich J.; Magalon, Hélène

doi:10.1007/s12686-015-0493-8

Cited by 10 publications

(9 citation statements)

References 1 publication

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“…DNA from 175 individuals was genotyped at 12 polymorphic microsatellite loci, Bd04, Bd18, Bd01, Bd02, Bd12, Bd09, Bd06, Bd20, Bd05, Bd03, Bd14, and Bd10 (Appendix ) (Basiita et al. in Goossens, ). Forward primers were fluorescently labeled using the 5‐dye system (6‐FAM, VIC, NED, PET, and LIZ GS‐500 size standard), and all reverse primers were pigtailed (Brownstein, Carpten, & Smith, ) to ensure consistent amplification and minimize stuttering.…”

Section: Methodsmentioning

confidence: 99%

“…A total of 20 μl PCR reactions were run on Biorad C1000 thermocycler under the following conditions: an initial denaturation at 95°C for 5 min, 6 cycles of 95°C for 30 s (denaturation)/59°C for 90 s (annealing)/72°C for 30 s (extension), 10 cycles at reduced annealing temperatures of 57, 55, and 53°C, prior to a final extension at 60°C for 30 min (Basiita et al. in Goossens, ). Visualization of PCR product was performed on an ABI‐3730 instrument (Applied Biosystems) using a 5‐standard dye system (6‐FAM, VIC, NED, PET, and LIZ GS‐500 size standard) at the Georgia Genomics Facility, USA.…”

Section: Methodsmentioning

confidence: 99%

“…Bagrus docmak (Ssemutundu) is a freshwater catfish with a widespread distribution in African freshwater rivers including the Nile, Chad, Niger, Volta, and Senegal. It is also found in Lake Victoria as well as the Rift Valley Lakes Edward, George, Albert, Tanganyika, Malawi, and Turkana (Aruho, Basiita, Kahwa, Bwanika, & Rutaisire, 2013;Golubtsov, Darkove, Dgebyadze, & Mina, 1995;Goossens, 2015;Greenwood, 1966;Mwanja et al, 2014). The fish species is potamodromous migrating from lakes to rivers during rainy seasons and from deep waters to shallow sandy bottoms to spawn (Chapman et al, 2012;Thieme et al, 2005).…”

Section: Introductionmentioning

confidence: 98%

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Populations genetically rifting within a complex geological system: The case of strong structure and low genetic diversity in the migratory freshwater catfish,Bagrus docmak,in East Africa

Basiita

Zenger

Jerry

2017

Ecology and Evolution

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The complex geological history of East Africa has been a driving factor in the rapid evolution of teleost biodiversity. While there is some understanding of how macroevolutionary drivers have shaped teleost speciation in East Africa, there is a paucity of research into how the same biogeographical factors have affected microevolutionary processes within lakes and rivers. To address this deficiency, population genetic diversity, demography, and structure were investigated in a widely distributed and migratory (potamodromous) African teleost species, Ssemutundu (Bagrus docmak). Samples were acquired from five geographical locations in East Africa within two major drainage basins; the Albertine Rift and Lake Victoria Basin. Individuals (N = 175) were genotyped at 12 microsatellite loci and 93 individuals sequenced at the mitochondrial DNA control region. Results suggested populations from Lakes Edward and Victoria had undergone a severe historic bottleneck resulting in very low nucleotide diversity (π = 0.004 and 0.006, respectively) and negatively significant Fu values (−3.769 and −5.049; p < .05). Heterozygosity deficiencies and restricted effective population size (N eLD) suggested contemporary exposure of these populations to stress, consistent with reports of the species decline in the East African Region. High genetic structuring between drainages was detected at both historical (ɸST = 0.62 for mtDNA; p < .001) and contemporary (microsatellite F ST = 0.460; p < .001) levels. Patterns of low genetic diversity and strong population structure revealed are consistent with speciation patterns that have been linked to the complex biogeography of East Africa, suggesting that these biogeographical features have operated as both macro‐ and micro‐evolutionary forces in the formation of the East African teleost fauna.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

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Populations genetically rifting within a complex geological system: The case of strong structure and low genetic diversity in the migratory freshwater catfish,Bagrus docmak,in East Africa

Basiita

Zenger

Jerry

2017

Ecology and Evolution

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“…PCR amplifi cation -Genetic differentiation was assessed using 14 plastid and 12 nuclear microsatellites (SSRs) ( Goossens, 2015 ;Basiita et al, 2016 ), as well as a 317 bp long section of the psbA-trnH intergenic spacer containing a variable 13 bp minisatellite motif (AGTCTAGATTTAG). PCR protocol and amplifi cation of the SSRs followed Basiita et al (2016) .…”

Section: Methodsmentioning

confidence: 99%

Hidden in plain view: Cryptic diversity in the emblematic Araucaria of New Caledonia

Ruhsam

Clark

Finger

et al. 2016

American J of Botany

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“…Importantly, the genetic marker used here was selected for the high resolution of mitochondrial loci, linked to their small effective population size and high sensitivity to sampling effects (Avise 2000, Avise 2004. Further studies will be needed to reveal how these patterns are reflected in nuclear or other genetic markers (for a valuable recent resource, see Goossens 2015).…”

Section: Current Haplotype Diversity In O Barnabitamentioning

confidence: 99%

Evidence for geographic substructuring of mtDNA variation in the East European Hermit beetle (Osmoderma barnabita)

Landvik¹,

Miraldo²,

Niemelä³

et al. 2017

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The genus Osmoderma is a flagship taxon of invertebrate conservation in Europe and encompasses a complex of four accepted species. While species limits amongst Osmoderma have been intensively studied, patterns of intraspecific variation are poorly known. In this paper, the authors focus on clarifying the phylogeographic structure of the East European Osmoderma barnabita using samples from Croatia to Finland. Samples of hind legs were collected from populations in Latvia and Finland (n=186) and combined with previously-published sequences from GenBank and museum specimens (n=10). In a partial sequence of the mitochondrial COI gene (759 bp), 26 closely related haplotypes were found. Beetle samples from different parts of Europe were distinct and showed no overlap in haplotype composition. The solitary population of Finland proved to be monomorphic and all 97 individuals sampled here belonged to a single haplotype unique to this region. The results suggest the Northern parts of Eastern Europe to be dominated * These authors contributed equally to this work. RESEARCH ARTICLE Launched to accelerate biodiversity conservation A peer-reviewed open-access journalMatti Landvik et al. / Nature Conservation 19: 171-189 (2017) 172 by a single COI haplotype to which most of the other haplotypes are linked by one or two mutations. The pattern seems to reflect a founder effect or a strong bottleneck event. While O. barnabita is widely distributed over Eastern Europe, current patterns of mitochondrial genetic diversity appear influenced by population history and little homogenisation by ongoing gene flow. From a conservation perspective, the patterns suggest that regional populations might need to be managed as subunits and that the population of Finland may be affected by low genetic diversity.

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Microsatellite records for volume 7, issue 4

Cited by 10 publications

References 1 publication

Populations genetically rifting within a complex geological system: The case of strong structure and low genetic diversity in the migratory freshwater catfish,Bagrus docmak,in East Africa

Populations genetically rifting within a complex geological system: The case of strong structure and low genetic diversity in the migratory freshwater catfish,Bagrus docmak,in East Africa

Hidden in plain view: Cryptic diversity in the emblematic Araucaria of New Caledonia

Evidence for geographic substructuring of mtDNA variation in the East European Hermit beetle (Osmoderma barnabita)

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