2005
DOI: 10.1007/s00251-005-0787-1
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Microsatellite typing of the rhesus macaque MHC region

Abstract: To improve the results gained by serotyping rhesus macaque major histocompatibility complex (MHC) antigens, molecular typing techniques have been established for class I and II genes. Like the rhesus macaque Mamu-DRB loci, the Mamu-A and -B are not only polymorphic but also polygenic. As a consequence, sequence-based typing of these genes is time-consuming. Therefore, eight MHC-linked microsatellites, or short tandem repeats (STRs), were evaluated for their use in haplotype characterization. Polymorphism analy… Show more

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Cited by 87 publications
(93 citation statements)
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“…Multiplex PCR assays were developed for 18 microsatellite loci spanning the MHC region (Table 1). Four of these loci were adapted for cynomolgus macaques from a previous study with rhesus macaques (31). Additional microsatellites were identified by screening human MHC primer pairs (15) for specificity against the rhesus MHC genomic sequence (9) with BLASTn (2).…”
Section: Methodsmentioning
confidence: 99%
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“…Multiplex PCR assays were developed for 18 microsatellite loci spanning the MHC region (Table 1). Four of these loci were adapted for cynomolgus macaques from a previous study with rhesus macaques (31). Additional microsatellites were identified by screening human MHC primer pairs (15) for specificity against the rhesus MHC genomic sequence (9) with BLASTn (2).…”
Section: Methodsmentioning
confidence: 99%
“…1). The majority of the primers used to amplify these markers were adapted to reflect rhesus genomic sequences (Table 1) (9,15,31). First, we used these markers to genotype DNA from five MCM previously shown to possess the MHC class I alleles Mafa-B*430101, Mafa-B*440101, and Mafa-B*460101 (20).…”
Section: Microsatellite Analysis Of Mauritian Cynomolgus Macaquesmentioning
confidence: 99%
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