Microsatellite variation in the freshwater schistosome‐transmitting snail Biomphalaria glabrata

Genetic differentiation, dispersal and mating system in the schistosome-transmitting freshwater snail Biomphalaria glabrata

“…This is consistent with previous studies in the same species (Mulvey and Vrijenhoek, 1982;Vianey-Liaud, 1997;Mavárez et al, 2000Mavárez et al, , 2002a. Moreover, this reasonably fits the bimodal distribution of selfing rates observed in freshwater snails, with most species being either preferential selfers or preferential outcrossers (see review in Städler and Jarne, 1997;and also Viard et al, 1997a, b;Mavárez et al, 2000aMavárez et al, , 2002aMeunier et al, 2001;Charbonnel et al, 2002;this study).…”

“…The variability was analysed at seven polymorphic microsatellite loci (Table 2) which were cloned in B. glabrata, and showed non-ambiguous amplification patterns. Detailed information about loci, PCR amplification and scoring can be found in Mavárez et al (2000) for loci BgC7, BgC8, BgE2, BgE4 and BgE5, and in Jones et al (1999) for loci BgU1 and BgU2. Briefly, individual microsatellite genotypes were obtained using either of two methods.…”

“…Briefly, individual microsatellite genotypes were obtained using either of two methods. In the first one, PCR amplification included ␥-33 P-labelled primers, and was followed by electrophoresis in acrylamide gels (Mavárez et al, 2000). The second method included PCR-amplification with fluorescent-labelled primers, and electrophoresis on a ABI 310 automatic genetic analyser using the manufacturer's protocols.…”

“…Little is therefore known on the demography and mating system of B. glabrata, and it is even possible that this taxon encompasses several genetically heterogeneous entities (Woodruff and Mulvey, 1997;Mavárez et al, 2002b). Microsatellite markers have recently been described in this species (Jones et al, 1999;Mavárez et al, 2000) and proved to be useful for smallscale genetic analyses of population structure and mating system in central Venezuela (Mavárez et al, 2002a). We here use these markers to describe the genetic diversity of B. glabrata at the scale of its distribution area, from Venezuela and the Lesser Antilles (north) to Brazil (south).…”

Genetic variability in Brazilian populations of Biomphalaria straminea complex detected by simple sequence repeat anchored polymerase chain reaction amplification

“…Jones et al (1999) reported the isolation and characterization of the first microsatellite loci in B. glabrata, and also demonstrated divergence between resistant and susceptible populations to S. mansoni. Mavárez et al (2000) characterized nine microsatellite loci in B. glabrata populations, from Venezuela, detecting at least eight suitable loci for studies on populational structure, reproduction systems and resistance to S. mansoni. Zietkiewicz et al (1994) introduced the simple sequence repeat anchored PCR (SSR-PCR) technique in the study of several eukariotic species.…”