1998
DOI: 10.1007/s001220050922
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Microsatellites in Cassava (Manihot esculenta Crantz): discovery, inheritance and variability

Abstract: Fourteen microsatellites containing GArepeats were isolated and characterized in cassava (Manihot esculenta Crantz, Euphorbiaceae). Microsatellite heterozygosity (h) was estimated in 48 accessions using (P)-end-labeled primers and in more than 500 accessions using fluorescence-based genotyping. Heterozygosity values ranged from 0.00 to 0.88 and the number of alleles detected varied from 1 to 15. The reproducibility of allele sizing was also assessed using fluorescence-based genotyping. The average inter-gel si… Show more

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Cited by 125 publications
(117 citation statements)
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“…Rocha et al (2008) have rectified this by a detailed examination of genetic diversity in Costa Rica using microsatellite loci. Their results demonstrated high levels of variation within and between populations, which corresponds with the results of other researchers using the same techniques for samples from South America (Chavarriaga et al 1998;Elias et al 2004) and is consistent with the large number of varieties per location and out-crossing between varieties. The high level of variation was in contrast to commercial varieties.…”
Section: The Internationalization Of Cassava Production and Contributsupporting
confidence: 89%
“…Rocha et al (2008) have rectified this by a detailed examination of genetic diversity in Costa Rica using microsatellite loci. Their results demonstrated high levels of variation within and between populations, which corresponds with the results of other researchers using the same techniques for samples from South America (Chavarriaga et al 1998;Elias et al 2004) and is consistent with the large number of varieties per location and out-crossing between varieties. The high level of variation was in contrast to commercial varieties.…”
Section: The Internationalization Of Cassava Production and Contributsupporting
confidence: 89%
“…Apesar do genoma completo do cloroplasto de mandioca ter sido descrito recentemente (DANIELL et al, 2008), ainda não foram desenvolvidos marcadores cpSSR para a espécie. Os marcadores ncSSR vêm sendo utilizados com diversos objetivos, como para quantificar a diversidade genética presente nas amostras de bancos de germoplasma e coleções nucleares (CHAVARRIAGA-AGUIRRE et al, 1998, 1999ELIAS et al, 2004;FREGENE et al, 2003; SIQUEIRA et al, 2009 -PEREIRA et al, 2011;DUPUTIÉ et al, 2009;McKEY, 2005;ROCHA et al, 2008;SARDOS et al, 2008).…”
Section: Marcadores Moleculares Microssatélitesunclassified
“…Na literatura atualmente existem 186 locos ncSSR descritos para mandioca (CHAVARRIAGA-AGUIRRE et al, 1998;MBA et al, 2001). Apesar do genoma completo do cloroplasto de mandioca ter sido descrito recentemente (DANIELL et al, 2008), ainda não foram desenvolvidos marcadores cpSSR para a espécie.…”
Section: Marcadores Moleculares Microssatélitesunclassified
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