Microsatellites to reveal genetic diversity and to distinguish four mangoes of Tegal District, Central Java, Indonesia

Abstract

Abstract. Hidayat A, Rahayu ES, Abdullah M, Retnoningsih A. 2021. Microsatellites to reveal genetic diversity and to distinguish four mangoes of Tegal District, Central Java, Indonesia. Biodiversitas 22: 3467-3473. The Wirasangka mango is Tegal District’s flora of identity, which requires recognition, and its existence deserves to be preserved. However, based on the morphology of the fruit, most of the people of Tegal still have difficulty distinguishing the wirasangka mango from other mangoes. Molecular markers are needed to ascertain the differences between these mango varieties. Microsatellite DNA is a repeating DNA of stable DNA markers with high repeatability and polymorphism. This study analyzes the level of genetic diversity and microsatellite alleles that can differentiate four mangoes from Tegal District, Central Java, Indonesia, i.e. wirasangka, tengkueh, golek, and okyong. Eleven accessions of six locations in Tegal District were analyzed using ten microsatellite loci. The microsatellite amplification result was separated using electrophoresis in 6% polyacrylamide gel and then visualized with silver dye. A total of 35 microsatellite alleles were found measuring 100-1000 bp, ranging from 1-7 alleles for each locus. The average polymorphism information content (PIC) of 0.54 indicates that genetic diversity is relatively high and informative. Therefore, the microsatellite alleles can be used to differentiate mango varieties. The specific allele characteristic of wirasangka mango accession is locus AJ63516, with an allele size of 600-700bp.

Molecular evaluation of selected mango genotypes grown in Egypt using SCoT and ISSR markers

“…The overall data (Figure 1 and Table 3) can be described as a total of 52 bands were produced, out of which 28 (53.84%) were polymorphic bands. Generally, the percent of polymorphism reported here was similar to that reported earlier in mangoes by Gitahi et al (2016) and Hidayat et al (2021), but lower than that reported by Surapaneni et al (2013), Zhou et al ( 2020), Anggraheni and Mulyaningsih (2021), He et al (2021) and Srivastav et al (2021). This polymorphism could be the result of replication slippage (Powell et al 1996) or it could be the result of the low genetic stability and high variability of the mango genome in the amplified non-coding regions (Fatima et al 2016).…”