Microscale methodology for structure elucidation of natural products

Molinski, Tadeusz F.

doi:10.1016/j.copbio.2010.09.003

Cited by 57 publications

(39 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As the natural pharmacopeia has grown and the preponderance of readily identified compounds has been catalogued, the long term success of secondary metabolite discovery campaigns has generally been determined by the degree to which this dependence on blind chance can be minimized. Historically, this has been accomplished via a number of strategies including the exploration of new ecologies, judicious selection of genera [14, 43, 69], and by the development of new analytical methodologies with improved analytical separation and sensitivity [55]. …”

Section: What Is Genome Mining and Why Is It Important?mentioning

confidence: 99%

Microbial genome mining for accelerated natural products discovery: is a renaissance in the making?

Bachmann

Lanen

Baltz³

2014

Journal of Industrial Microbiology and Biotechnology

250

187

View full text Add to dashboard Cite

Microbial genome mining is a rapidly developing approach to discover new and novel secondary metabolites for drug discovery. Many advances have been made in the past decade to facilitate genome mining, and these are reviewed in this Special Issue of the Journal of Industrial Microbiology and Biotechnology. In this Introductory Review, we discuss the concept of genome mining and why it is important for the revitalization of natural product discovery; what microbes show the most promise for focused genome mining; how microbial genomes can be mined; how genome mining can be leveraged with other technologies; how progress on genome mining can be accelerated; and who should fund future progress in this promising field. We direct interested readers to more focused reviews on the individual topics in this Special Issue for more detailed summaries on the current state-of-the-art.

show abstract

Section: What Is Genome Mining and Why Is It Important?mentioning

confidence: 99%

Microbial genome mining for accelerated natural products discovery: is a renaissance in the making?

Bachmann

Lanen

Baltz³

2014

Journal of Industrial Microbiology and Biotechnology

250

187

View full text Add to dashboard Cite

show abstract

“… 9-O-Desmethylkabiramide B (620 g) 275,276  Sanguinamides A and B (390 g and 190 g, respectively) 275,276 .…”

Section: Box 2: Advances In Nmr Techniquesmentioning

confidence: 99%

“… Phorbaside F (7.5 g), 275,276  Hemi-phorboxazole A (16.5 g) 275,276  Muironolide A (90 g) [275][276][277] .…”

Section: Box 2: Advances In Nmr Techniquesmentioning

confidence: 99%

The re-emergence of natural products for drug discovery in the genomics era

Harvey

Edrada-Ebel

Quinn

2015

Nat Rev Drug Discov

2,114

1,410

View full text Add to dashboard Cite

Natural products have been a rich source of compounds for drug discovery. However, their use has diminished in the past two decades in part because of technical barriers to screening natural products in high-throughput assays against molecular targets. Here, we review advances in chemical techniques for the isolation and structural elucidation of natural products that have reduced these technological barriers. We also assess the use of genomic and metabolomic approaches to augment traditional methods of studying natural products, and highlight recent examples of natural products in antimicrobial drug discovery and as inhibitors of protein protein interactions. The growing appreciation of functional assays and phenotypic screens may further contribute to a revival of interest in natural products for drug discovery.

show abstract

“…The Molinski group developed a simple and accurate method for quantitation of small amounts of natural products, taking advantages of the developed technologies in microcoil probes and narrow diameter tube microprobes [86]. They used the solvent 13 C-satellites (QSCS) method for the quantitation, which uses the integration of 13 C satellite peaks of deuterated solvents, in particular After establishing the proportionality constant, c, in a specific spectrometer, we can apply Eq.…”

Section: Quantitative Nmrmentioning

confidence: 99%