Microscale pressure measurements based on an immiscible fluid/fluid interface

Yang, Jing; Duan, Xing; Fraser, Andrew K.; Choudhury, Mohammad Ikbal; Ewald, Andrew J.; Li, Rong; Sun, Sean X.

doi:10.1038/s41598-019-56573-x

Cited by 9 publications

(5 citation statements)

References 24 publications

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“…Indeed, this force, in the form of an apical-basal hydraulic pressure difference, was quantified for kidney epithelium using a permeable microfluidic method (Choudhury et al, 2019 preprint). Similarly, MDCK II cells grown on impermeable substrates can generate dynamic fluid-fill domes (Yang et al, 2019). These domes are generated from active pumping of water across the epithelium into the domes, and according to OEM predictions, the hydraulic pressure is higher inside the dome.…”

Section: Water Dynamics In Tissuesmentioning

confidence: 99%

“…This lumen expansion has been shown to rely on elevated pressure inside the lumen. Another example is the mammary gland organoid; here, expansion of the organoid in the matrix and generation of a fluidfilled lumen also requires an elevated pressure (Yang et al, 2019). The elevated pressure is not particularly highof the order 100-300 Pa, which is about 0.1-0.3% of the atmospheric pressure.…”

Section: Water Dynamics In Tissuesmentioning

confidence: 99%

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The importance of water and hydraulic pressure in cell dynamics

Κωνσταντόπουλος

Zhao

et al. 2020

Journal of Cell Science

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All mammalian cells live in the aqueous medium, yet for many cell biologists, water is a passive arena in which proteins are the leading players that carry out essential biological functions. Recent studies, as well as decades of previous work, have accumulated evidence to show that this is not the complete picture. Active fluxes of water and solutes of water can play essential roles during cell shape changes, cell motility and tissue function, and can generate significant mechanical forces. Moreover, the extracellular resistance to water flow, known as the hydraulic resistance, and external hydraulic pressures are important mechanical modulators of cell polarization and motility. For the cell to maintain a consistent chemical environment in the cytoplasm, there must exist an intricate molecular system that actively controls the cell water content as well as the cytoplasmic ionic content. This system is difficult to study and poorly understood, but ramifications of which may impact all aspects of cell biology from growth to metabolism to development. In this Review, we describe how mammalian cells maintain the cytoplasmic water content and how water flows across the cell surface to drive cell movement. The roles of mechanical forces and hydraulic pressure during water movement are explored.

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Section: Water Dynamics In Tissuesmentioning

confidence: 99%

Section: Water Dynamics In Tissuesmentioning

confidence: 99%

The importance of water and hydraulic pressure in cell dynamics

Κωνσταντόπουλος

Zhao

et al. 2020

Journal of Cell Science

Self Cite

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“…In addition to microelectrodes, 17 glass needles equipped with pressure sensors 19 have been used, or pressure was measured based on the shape of the interface between the oil and body fluid inside a glass needle. 49 To measure intracellular pressure, a nanomechanical device with Fabry-Pérot cavity or nanoparticles that exhibit fluorescence spectrum changes in response to pressure have been developed. 50 , 51 , 52 Indirect methods were also developed to estimate the pressure in a cavity following a shape change that occurs when a force is applied to the cavity wall.…”

Section: Discussionmentioning

confidence: 99%

Blastopore gating mechanism to regulate extracellular fluid excretion

Kato¹,

Inomata²

2023

iScience

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“…It is important to note that making microscale pressure measurements in a closed lumen is very challenging. The pressure range in lumens, epithelial domes, and developing mouse embryos, which generally falls between 100 and 300 Pa, can be measured through curvature changes in a fluid/fluid interface as described by Yang et al [226]. Conventional organoids and spheroids form closed lumens, making them inaccessible to measurements of fluid flow and shear stress.…”

Section: Control Of the Mechanical Environmentmentioning

confidence: 99%

Using Biosensors to Study Organoids, Spheroids and Organs-on-a-Chip: A Mechanobiology Perspective

Yousafzai,

Hammer

2023

Biosensors

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The increasing popularity of 3D cell culture models is being driven by the demand for more in vivo-like conditions with which to study the biochemistry and biomechanics of numerous biological processes in health and disease. Spheroids and organoids are 3D culture platforms that self-assemble and regenerate from stem cells, tissue progenitor cells or cell lines, and that show great potential for studying tissue development and regeneration. Organ-on-a-chip approaches can be used to achieve spatiotemporal control over the biochemical and biomechanical signals that promote tissue growth and differentiation. These 3D model systems can be engineered to serve as disease models and used for drug screens. While culture methods have been developed to support these 3D structures, challenges remain to completely recapitulate the cell–cell and cell–matrix biomechanical interactions occurring in vivo. Understanding how forces influence the functions of cells in these 3D systems will require precise tools to measure such forces, as well as a better understanding of the mechanobiology of cell–cell and cell–matrix interactions. Biosensors will prove powerful for measuring forces in both of these contexts, thereby leading to a better understanding of how mechanical forces influence biological systems at the cellular and tissue levels. Here, we discussed how biosensors and mechanobiological research can be coupled to develop accurate, physiologically relevant 3D tissue models to study tissue development, function, malfunction in disease, and avenues for disease intervention.

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Microscale pressure measurements based on an immiscible fluid/fluid interface

Cited by 9 publications

References 24 publications

The importance of water and hydraulic pressure in cell dynamics

The importance of water and hydraulic pressure in cell dynamics

Blastopore gating mechanism to regulate extracellular fluid excretion

Using Biosensors to Study Organoids, Spheroids and Organs-on-a-Chip: A Mechanobiology Perspective

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