In this study, the importance of the accuracy of the thermophysical property to a simulation of an investment cast directionally solidified nickel-based superalloy turbine blade was investigated. CMSX-4 was chosen as the base alloy. The effect of the thermophysical properties on the thermal field and solidification microstructure was studied using a coupled macromesoscale model. Relevant thermophysical properties examined include density, specific heat (enthalpy) and thermal conductivity in both the liquid and the solid phases. Commercial finite-element analysis software, ProCAST, was used as the macroscale model to solve the heat and mass transportation equations. An in-house mesoscale code was used to simulate the dendritic solidification structure including microsegregation. During the macroscale analysis, the simulation results were compared to an idealized turbine blade previously cast under industrial conditions. In the mesoscale simulation, the response of primary arm spacing and segregation range was examined. The temperature prediction and the thermal gradient in the liquid show the greatest sensitivity to density and solid state thermal conductivity. Primary arm spacing is most sensitive to density and specific heat. Segregation range shows greatest sensitivity to density and thermal diffusivity in the solid.