2022
DOI: 10.1007/978-1-0716-2413-5_15
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Microscale Thermophoresis to Study RNA–RNA Binding Affinity

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“…Consequently, the binding affinity further validates our AUC experiment, which was performed with a ∼200 nM concentration of JEV TR RNA. To our knowledge, few studies ( 55 , 56 ) have shown evidence of RNA–RNA interactions characterized using MST, highlighting the growing applicability of the technique. Next, we needed to validate whether the CS is the primary driver of this RNA–RNA interaction.…”
Section: Resultsmentioning
confidence: 99%
“…Consequently, the binding affinity further validates our AUC experiment, which was performed with a ∼200 nM concentration of JEV TR RNA. To our knowledge, few studies ( 55 , 56 ) have shown evidence of RNA–RNA interactions characterized using MST, highlighting the growing applicability of the technique. Next, we needed to validate whether the CS is the primary driver of this RNA–RNA interaction.…”
Section: Resultsmentioning
confidence: 99%