Microscopic analysis of colonization of Colletotrichum abscissum in citrus tissues

Savi, Daiani Cristina; Rossi, Bruno Janoski; Rossi, Gustavo Rodrigues; Ferreira-Maba, Lisandra Santos; Bini, Israel Henrique; Trindade, Edvaldo da Silva; Goulin, Eduardo Henrique; Machado, Marcos Antônio; Glienke, Chirlei

doi:10.1016/j.micres.2019.05.005

Cited by 8 publications

(9 citation statements)

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“…In addition to the inhibition of mycelial growth, the isolate D. vochysiae CMRP4322 also inhibited the germination of C. abscissum conidia at lower than 1 μg/mL concentrations. These results are quite interesting due to the PFD cycle caused by the production of conidia in already infected flowers, which are then splashed on surrounding leaves, twigs and floral buds that remain after the blossom period ( Agostini et al, 1992 ; Silva-junior et al, 2014 ; Pinho et al, 2015 ; Silva A. O. et al, 2016 ; Savi et al, 2019b ). In Brazil, PDF disease leads to large losses in citrus production, estimated 80%, when rainy periods occur during the bloom phase ( Goes et al, 2008 ; Silva A. O. et al, 2016 ).…”

Section: Discussionmentioning

confidence: 99%

Endophytes of Brazilian Medicinal Plants With Activity Against Phytopathogens

et al. 2021

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Plant diseases caused by phytopathogens are responsible for significant crop losses worldwide. Resistance induction and biological control have been exploited in agriculture due to their enormous potential. In this study, we investigated the antimicrobial potential of endophytic fungi of leaves and petioles of medicinal plants Vochysia divergens and Stryphnodendron adstringens located in two regions of high diversity in Brazil, Pantanal, and Cerrado, respectively. We recovered 1,304 fungal isolates and based on the characteristics of the culture, were assigned to 159 phenotypes. One isolate was selected as representative of each phenotype and studied for antimicrobial activity against phytopathogens. Isolates with better biological activities were identified based on DNA sequences and phylogenetic analyzes. Among the 159 representative isolates, extracts from 12 endophytes that inhibited the mycelial growth (IG) of Colletotrichum abscissum (≥40%) were selected to expand the antimicrobial analysis. The minimum inhibitory concentrations (MIC) of the extracts were determined against citrus pathogens, C. abscissum, Phyllosticta citricarpa and Xanthomonas citri subsp. citri and the maize pathogen Fusarium graminearum. The highest activity against C. abscissum were from extracts of Pseudofusicoccum stromaticum CMRP4328 (IG: 83% and MIC: 40 μg/mL) and Diaporthe vochysiae CMRP4322 (IG: 75% and MIC: 1 μg/mL), both extracts also inhibited the development of post-bloom fruit drop symptoms in citrus flowers. The extracts were promising in inhibiting the mycelial growth of P. citricarpa and reducing the production of pycnidia in citrus leaves. Among the isolates that showed activity, the genus Diaporthe was the most common, including the new species D. cerradensis described in this study. In addition, high performance liquid chromatography, UV detection, and mass spectrometry and thin layer chromatography analyzes of extracts produced by endophytes that showed high activity, indicated D. vochysiae CMRP4322 and P. stromaticum CMRP4328 as promising strains that produce new bioactive natural products. We report here the capacity of endophytic fungi of medicinal plants to produce secondary metabolites with biological activities against phytopathogenic fungi and bacteria. The description of the new species D. cerradensis, reinforces the ability of medicinal plants found in Brazil to host a diverse group of fungi with biotechnological potential.

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Section: Discussionmentioning

confidence: 99%

Endophytes of Brazilian Medicinal Plants With Activity Against Phytopathogens

et al. 2021

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“…Although PFD symptoms are not observed on citrus leaves, C . abscissum may produce appressoria on the leaf surface and survive on citrus and weed leaves (Agostini & Timmer, 1994; Frare et al, 2016; Savi et al, 2019; Zulfiqar et al, 1996). In the greenhouse, C .…”

Section: Introductionmentioning

confidence: 99%

“…Under the same controlled conditions, C . abscissum did not colonize citrus leaves, with or without injury, but the pathogen produced a large amount of secondary conidia on the leaf surface, which may be an important mechanism for its spread (Savi et al, 2019). However, quiescent infections by C .…”

Section: Introductionmentioning

confidence: 99%

“…Despite both Colletotrichum species causing PFD, only C. gloeosporioides sensu stricto shows endophytic colonization in the sweet orange leaves (Waculicz-Andrade et al, 2017). Although PFD symptoms are not observed on citrus leaves, C. abscissum may produce appressoria on the leaf surface and survive on citrus and weed leaves Frare et al, 2016;Savi et al, 2019;Zulfiqar et al, 1996). In the greenhouse, C. abscissum was shown to survive for up to 7 months on leaves with intermittent simulated rainfall or only 3 months without rainfall; in the field, the pathogen was isolated from leaves for 7 months in rainy periods and 3 months in the dry season (Pereira, 2013).…”

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confidence: 99%

“…In the greenhouse, C. abscissum was shown to survive for up to 7 months on leaves with intermittent simulated rainfall or only 3 months without rainfall; in the field, the pathogen was isolated from leaves for 7 months in rainy periods and 3 months in the dry season (Pereira, 2013). Under the same controlled conditions, C. abscissum did not colonize citrus leaves, with or without injury, but the pathogen produced a large amount of secondary conidia on the leaf surface, which may be an important mechanism for its spread (Savi et al, 2019). However, quiescent infections by C. abscissum in leaf tissues have already been reported by some authors as likely to occur (Timmer & Peres, 2015), although the possibility of citrus endophytic colonization by C. abscissum was rejected by Waculicz-Andrade et al (2017).…”

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