Microscopic and Molecular Detection of Theileria annulata Infection of Cattle in Egypt

El‐Dakhly, Khaled Mohamed; Arafa, Waleed M.; Ghanem, Saad Soliman; Abdel-Fatah, Omima Ramadan; Wahba, Ahmed A.

doi:10.17582/journal.jap/2018/5.2.29.34

Cited by 12 publications

(10 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In blood smear examination the highest positive rate was 38.9%, the lowest rate was 5.1%, and the relative average positive rate was 13.7% Proved by (Li et al 2016). Microscopic examination of Giemsastained thin blood smears 9.31% and PCR 11.44% (El-Dakhly et al, 2018). The sero-prevalence of T. annulata in cattle was 10.6% (21 out of 198) in Central Anatolia, Turkey (Sayin et al, 2003).…”

Section: Discussionmentioning

confidence: 99%

Cattle Theileriosis: Prevalence and Comparative by Using ELISA and Blood Smears Techniques in Sulaimani Province - Kurdistan Region / Iraq.

Mustafa

2020

Journal of Animal and Poultry Production

View full text Add to dashboard Cite

Isolated one hundred fifty adult female cattle (local breed) one hundred infested with a hard tick (Ixodidae) and show some clinical signs for a search of theileriosis such as; enlargement pre-scapular lymph node and pale of the eye mucus membrane, fifty of them use a control (Non infested cattle) during the period of April to November 2018 in Sulaimani province. Indicated the highest sensitivity (prevalence) of Theileria annulata when examined by ELISA technique 63% (63/100), the prevalence rate of macroscopically detection to T. annulata merozoite was 48% as the following, in mixed lymph node biopsy cells and blood smears technique was 22%, the blood and lymph node biopsy smear examinations were 15% and 11% respectively. Distinguished the total prevalence rate in control cattle was 6% and 4% in the ELISA technique and microscopically method respectively. Sensitivity values and positive predictive noted a highly in ELISA serolog-ical test 63% and 95.45% respectively, while in mixed lymph node biopsy and blood smears examined 22% and 100% respectively. During the study collected 1368 hard tick and identified to three genera in infested cattle of Ixodidae; Hyalomma species are the highest number percentage (44.8%), followed Boophilus species (28.6%) and the Rhipicephalus species is the lowest percentage (26.6%). This study conducted to find out the prevalence the local cattle with theileriosis (T. annulata) by the microscopic examination for both blood and lymph biopsy smear (merozoite) and compared with the (ELISA) technique.

show abstract

Section: Discussionmentioning

confidence: 99%

Cattle Theileriosis: Prevalence and Comparative by Using ELISA and Blood Smears Techniques in Sulaimani Province - Kurdistan Region / Iraq.

Mustafa

2020

Journal of Animal and Poultry Production

View full text Add to dashboard Cite

show abstract

“…Theileriosis commonly spreads among cattle in the tropical and subtropical regions and is prevalent worldwide. It spreads from Asia through the Middle East [ 5 ] to North Africa [ 6 ] and South Europe [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

Identification and discrimination of Theileria annulata by polymerase chain reaction-restriction fragment length polymorphism

Selim

Khater

2022

Vet World

View full text Add to dashboard Cite

Background and Aim: Theileria annulata infection is a tick-borne disease affecting ruminants in the tropical and subtropical regions causing severe economic losses. This study aimed to characterize circulating T. annulata isolates from four governorates (administrative districts) north and south of Egypt using polymerase chain reaction (PCR)-restriction fragment length polymorphism. Materials and Methods: Fifty samples were collected from the four governorates of Egypt and were examined by a PCR assay based on the heat shock protein 70 gene. The amplified product was subsequently digested using two restriction enzymes, Taq I and Alu I, to determine which pattern of T. annulata strains was involved. Results: The findings revealed that one distinct pattern was observed for T. annulata isolates in the northern governorates and another one in the southern governorates. The Taq I enzyme produced three fragments (100, 175, and 270 bp), and the Alu I enzyme produced four fragments (60, 90, 125, and 270 bp). Conclusion: This study determined the presence of two distinct circulating genotypes of T. annulata among cattle in Egypt based on PCR-RFLP using the HSP 70 gene. More studies are needed in different parts of the country to investigate the virulence and strain variance of T. annulata in cattle.

show abstract

“…Theileria annulata, the pathogen causing bovine tropical theileriosis, leads to acute lymphoproliferative disease in bovines that has a number of clinical features in common with human leukemias [1]. The disease poses a major threat to cattle in subtropical and tropical countries, including Africa, Europe, the Mediterranean basin, the Middle East and Asia [2][3][4][5][6][7][8]. Tropical theileriosis also causes substantial economic loss in the livestock industry…”

Section: Introductionmentioning

confidence: 99%

Theileria annulata SVSP455 interacts with host HSP60

Liu

Zhao

et al. 2022

Parasites Vectors

View full text Add to dashboard Cite

Background Theileria annulata, a transforming parasite, invades bovine B cells, dendritic cells and macrophages, promoting the uncontrolled proliferation of these cells. This protozoan evolved intricate strategies to subvert host cell signaling pathways related to antiapoptotic signaling to enable survival and proliferation within the host cells. However, the molecular mechanisms of the cell transformation induced by T. annulata remain largely unclear. Although some studies have predicted that the subtelomere-encoded variable secreted protein (SVSP) family plays roles in host-parasite interactions, the evidence for this is limited. Methods In the present study, the SVSP455 (TA05545) gene, a member of the SVSP gene family, was used as the target molecule. The expression pattern of SVSP455 in different life-cycle stages of T. annulata infection was explored using a quantitative real-time PCR assay, and the subcellular distribution of SVSP455 was observed using confocal microscopy. The host cell proteins interacting with SVSP455 were screened using the Y2H system, and their interactions were verified in vivo and in vitro using both bimolecular fluorescence complementation and confocal microscopy, and co-immunoprecipitation assays. The role played by SVSP455 in cell transformation was further explored by using overexpression, RNA interference and drug treatment experiments. Results The highest level of the SVSP455 transcript was detected in the schizont stage of T. annulata, and the protein was located both on the surface of schizonts and in the host cell cytoplasm. In addition, the interaction between SVSP455 and heat shock protein 60 was shown in vitro, and their link may regulate host cell apoptosis in T. annulata-infected cells. Conclusion Our findings are the first to reveal that T. annulata-secreted SVSP455 molecule directly interacts with both exogenous and endogenous bovine HSP60 protein, and that the interaction of SVSP455-HSP60 may manipulate the host cell apoptosis signaling pathway. These results provide insights into cancer-like phenotypes underlying Theilera transformation and therapeutics for protection against other pathogens. Graphical Abstract

show abstract

Microscopic and Molecular Detection of Theileria annulata Infection of Cattle in Egypt

Cited by 12 publications

References 15 publications

Cattle Theileriosis: Prevalence and Comparative by Using ELISA and Blood Smears Techniques in Sulaimani Province - Kurdistan Region / Iraq.

Cattle Theileriosis: Prevalence and Comparative by Using ELISA and Blood Smears Techniques in Sulaimani Province - Kurdistan Region / Iraq.

Identification and discrimination of Theileria annulata by polymerase chain reaction-restriction fragment length polymorphism

Theileria annulata SVSP455 interacts with host HSP60

Contact Info

Product

Resources

About