Microscopic considerations for optimizing silk biomaterials

DeBari, Megan K.; Abbott, Rosalyn D.

doi:10.1002/wnan.1534

Cited by 35 publications

(38 citation statements)

References 92 publications

(147 reference statements)

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“…The associated high demands (functional and regulatory) placed on scaffolds have limited their success. Indeed, a scaffold (Griffith and Naughton, 2002;DeBari and Abbott, 2019) need to be biocompatible, have suitable mechanical properties, be permeable to fluids, and easy to manufacture to be commercially successful (Howard et al, 2008). Additionally, the scaffolds must facilitate angiogenesis (the ingrowth of blood vessels) once implanted (Varley et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

“…Generally, larger interconnected pores (300-500 µm) promotes good exchange of nutrients, but not necessarily an adequate cellular growth, while smaller pores (<50 µm) can promote adequate cell adhesion and short-term proliferation (Griffon et al, 2006;Ribeiro et al, 2018Ribeiro et al, , 2019. The interconnection and orientation of the pores are yet to be achieved and understood (Zhu et al, 2014;Qi et al, 2017;DeBari and Abbott, 2019). Mathematical modeling is also a challenge.…”

Section: Introductionmentioning

confidence: 99%

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3D Structure and Mechanics of Silk Sponge Scaffolds Is Governed by Larger Pore Sizes

et al. 2020

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Three-dimensional scaffolds play an essential role in tissue engineering. Although essential, the tunability of the 3D scaffolds mechanical and transport properties remains a challenge. In this work, we present new approaches to advance the field. First, we applied our progressive pH acidification to mimic the natural silk gelation process before ice-templating (−20 and −80 • C); second, we fitted the mechanical properties using a connectivity model; third, we fitted the scaffolds mechanical relaxation to understand the transport properties; and fourth we used micro-CT to correlate the process parameters to the scaffolds' performances. Our results suggested that the free shrinkage of the scaffolds determined their final properties. We found, however, that the porosity (above 90%) was anisotropic, similarly the tortuosity (between 1 and 1.3). We identified two major pore dimensions, the first one between 10 and 20 µm, and the second between 50 and 130 µm. Mechanically, our model suggested that the bulk modulus captured the elastic contribution and was controlled predominantly by the silk concentration. We tentatively associated the fractional modulus 1 to the collapse of the larger pores structures and was controlled mostly by the process temperature. We assigned the slow relaxation to the transport of fluid in the silk sponge scaffolds; and the fast relaxation with a viscoelastic relaxation. The silk concentration and process temperatures did not influence the latter. Overall, our use of the tomography, mechanical test, and detailed statistical analysis provides inroads into the interplay between process parameters (silk concentration and process temperature) and the multiple responses of the silk sponge scaffolds. The development of a new mechanical fitting for the compression test helped capture simply the different failure modes in the sponge scaffolds as well as correlating those events to relaxation and eventually transport properties.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

3D Structure and Mechanics of Silk Sponge Scaffolds Is Governed by Larger Pore Sizes

et al. 2020

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“…This process only breaks the amide bond of SS, but retains the intact SF. [ 115 ] Hence, it is also a suitable SS extraction method for A. mylitta cocoons. Compared with methods using high temperature or alkaline treatments, the chemical gentle LiBr method maximizes the integrity of SS.…”

Section: Silk Of Silkworms: Composition Structure and Propertiesmentioning

confidence: 99%

Silk‐Based Biomaterials for Cardiac Tissue Engineering

Song

Wang

Yue

et al. 2020

Adv Healthcare Materials

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Cardiovascular diseases are one of the leading causes of death globally. Among various cardiovascular diseases, myocardial infarction is an important one. Compared with conventional treatments, cardiac tissue engineering provides an alternative to repair and regenerate the injured tissue. Among various types of materials used for tissue engineering applications, silk biomaterials have been increasingly utilized due to their biocompatibility, biological functions, and many favorable physical/chemical properties. Silk biomaterials are often used alone or in combination with other materials in the forms of patches or hydrogels, and serve as promising delivery systems for bioactive compounds in tissue engineering repair scenarios. This review focuses primarily on the promising characteristics of silk biomaterials and their recent advances in cardiac tissue engineering.

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“…Core fibroin is covered by glue‐like protein known as sericin which helps the fiber to glue together in cocoon as shown in Figure a. Sericine is rich with amino acid serine with a molecular weight ranges between 10 and 300 kDa . It is antibacterial and UV‐resistant.…”

Section: Silk and Fibroin Sericinmentioning

confidence: 99%

Overview of Protein‐Based Biopolymers for Biomedical Application

Nagarajan

Radhakrishnan

Kalkura

et al. 2019

Macro Chemistry & Physics

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Biopolymers are playing a vital role in biomedical applications. Among them, protein‐based biopolymers are utilized for the fabrication of tissue‐engineering constructs, therapeutic molecule delivery carriers, emulsifiers, and food packaging materials. Wide ranges of proteins are extracted from animal or plant sources and are being utilized for the fabrication of scaffolds for regenerative tissue‐engineering application. Here, an overview about the protein structure, extraction procedure, solubility, and various formulation‐based proteins found in the literature are discussed. Biopolymers display several advantages such as biocompatibility and degradability by enzymes. Methods to overcome the disadvantages of these proteins such as immunogenicity, antigenicity, and solubility are reported. Various crosslinking reagents specific to protein chemistry are discussed as well.

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Microscopic considerations for optimizing silk biomaterials

Cited by 35 publications

References 92 publications

3D Structure and Mechanics of Silk Sponge Scaffolds Is Governed by Larger Pore Sizes

3D Structure and Mechanics of Silk Sponge Scaffolds Is Governed by Larger Pore Sizes

Silk‐Based Biomaterials for Cardiac Tissue Engineering

Overview of Protein‐Based Biopolymers for Biomedical Application

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