Microscopic Detection of Thermogenesis in a Single HeLa Cell

Suzuki, Madoka; Tseeb, Vadim; Oyama, Kotaro; Ishiwata, Shin’ichi

doi:10.1529/biophysj.106.098673

Cited by 161 publications

(159 citation statements)

References 16 publications

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“…1SU4 is a conformation where Ca 2+ is present (E1 state), whereas 1KJU is a conformation corresponding to the Ca 2+ -free (E2) state. It has been recently found that ATPases release heat when they are transporting Ca 2+ , 8 hence knowledge of the thermal transport properties is needed to characterize the heat transfer in these enzymes. The protein interactions were modeled using the GROMOS96 43a2 force field.…”

Section: Computer Simulations Of the Proteinsmentioning

confidence: 99%

Heat transfer in protein–water interfaces

Lervik

Bresme

Kjelstrup

et al. 2010

Phys. Chem. Chem. Phys.

102

135

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We investigate using transient non-equilibrum molecular dynamics simulation the temperature relaxation process of three structurally different proteins in water, namely; myoglobin, green fluorescence protein (GFP) and two conformations of the Ca 2+ -ATPase protein. By modeling the temperature relaxation process using the solution of the heat conduction equation we compute the thermal conductivity and thermal diffusivity of the proteins, as well as the thermal conductance of the protein-water interface. Our results indicate that the temperature relaxation of the protein can be described using a macroscopic approach. The protein-water interface has a thermal conductance of the order of 100-270 MW m −2 K −1 , characteristic of water-hydrophilic interfaces. The thermal conductivity of the proteins is of the order of 0.1-0.2 † Imperial College and NTNU ‡ Imperial College and CAS ¶ NTNU and CAS § UB and CAS 1 Anders Lervik et al.Heat transfer in protein-water interfaces W m −1 K −1 as compared with ≈ 0.6 W m −1 K −1 for water, suggesting that these proteins can develop temperature gradients within the biomolecular structures that are larger than those of aqueous solutions. We find that the thermal diffusivity of the transmembrane protein, Ca 2+ -ATPase is about three times larger than that of myoglobin or GFP. Our simulation shows that the Kapitza length of these structurally different proteins is of the order of 1 nm, showing that the protein-water interface should play a major role in defining the thermal relaxation of biomolecules.

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Section: Computer Simulations Of the Proteinsmentioning

confidence: 99%

Heat transfer in protein–water interfaces

Lervik

Bresme

Kjelstrup

et al. 2010

Phys. Chem. Chem. Phys.

102

135

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“…[1][2][3][4] Accurately measuring intracellular temperature may provide new insights into cellular signaling and metabolism. However, sensing intracellular temperature is not a trivial task, especially at the single-cell level.…”

Section: Introductionmentioning

confidence: 99%

“…However, sensing intracellular temperature is not a trivial task, especially at the single-cell level. 3 Currently, there are two major approaches to intracellular temperature sensing: one uses micro-or nano-scale thermocouples 1,5 ; the other employs temperature-sensitive fluorescent dyes, 6,7 proteins, 8 or nanoparticles. 9,10 Although the thermocouple-based approach features high-temperature resolution (∼0.1°C), 5 to detect intracellular temperature changes, the tip of the thermocouple has to be inserted into cells via a micromanipulation system.…”

Section: Introductionmentioning

confidence: 99%

Single-cell photoacoustic thermometry

Gao

Wang

et al. 2013

J. Biomed. Opt

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Abstract. A novel photoacoustic thermometric method is presented for simultaneously imaging cells and sensing their temperature. With three-seconds-per-frame imaging speed, a temperature resolution of 0.2°C was achieved in a photo-thermal cell heating experiment. Compared to other approaches, the photoacoustic thermometric method has the advantage of not requiring custom-developed temperature-sensitive biosensors. This feature should facilitate the conversion of single-cell thermometry into a routine lab tool and make it accessible to a much broader biological research community.

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“…Thus, several approaches have been proposed to detect cellular temperature response using the emission intensity or lifetime of organic dyes (Chapman et al, 1995;Gota et al, 2009) and transition metal ions (Zohar et al, 1998;Suzuki et al, 2007). These pioneering works were able to provide the average temperature for individual cells.…”

Section: Approaches To Monitoring Of Intracellular Temperaturementioning

confidence: 99%

Cell Thermoregulation: Problems, Advances and Perspectives

Ibraimov¹

2017

JMBR

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Thermoregulation at organism level is the well-established fact. The question on possibility of thermoregulation at the cell level remains opened. Based on study of distribution of chromosomal heterochromatin regions (HRs) in various human populations, in norm and at some forms of pathology the hypothesis about thermoregulation existence at the cell level has been presented. The essence of hypothesis of cell thermoregulation (СТ) is elimination of the temperature difference between the nucleus and cytoplasm when the nucleus temperature becomes higher than the cytoplasm temperature. The nucleus, in contrast to the cytoplasm, cannot conduct heat directly in the extracellular space, from where the heat is taken by the circulating flow of sap, lymph and blood. Thus, the nucleus can conduct heat only in the cytoplasm. With this, the nucleus has two options for the dissipation of heat surplus: either by increasing its volume or increasing the heat conductivity of the nuclear envelope. As the first option is limited, and the second one is hampered because of thickness of the cell membranes, apparently the higher eukaryotes took advantage of the opportunity of a dense layer of peripheral condensed chromatin (CC) as heat conductor for a more efficient elimination of the temperature difference between the nucleus and cytoplasm. The СС localized between a nucleus and cytoplasm is made of different types of chromosomal HRs. For this reason, СС is subject to wide variability in population. Obviously, the density of the СС packing depends on the type and quantity of chromosomal HRs in its structure that can affect upon its heat-conducting ability. If the situation is this then we are entitled to expect a new type of variability with all consequences resulting from here.

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Microscopic Detection of Thermogenesis in a Single HeLa Cell

Cited by 161 publications

References 16 publications

Heat transfer in protein–water interfaces

Heat transfer in protein–water interfaces

Single-cell photoacoustic thermometry

Cell Thermoregulation: Problems, Advances and Perspectives

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