Microscopic heat pulses activate cardiac thin filaments

Ishii, Shuya; Oyama, Kotaro; Arai, Tomomi; Itoh, Hideki; Shintani, Seine A.; Suzuki, Madoka; Kobirumaki-Shimozawa, Fuyu; Terui, Takako; Fukuda, Norio; Ishiwata, Shin’ichi

doi:10.1085/jgp.201812243

Cited by 15 publications

(38 citation statements)

References 58 publications

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“…Brunet et al (2012) analyzed sliding movements of thin filaments that had been reconstituted with human cTn and Tm at temperatures above ∼43 • C under relaxing conditions in the absence of Ca 2+ (+EGTA). We performed a rapid-heating experiment using infrared laser irradiation and found that thin filaments that had been reconstituted with bovine cTn and human Tm exhibited sliding movements at >∼35 • C in the absence of Ca 2+ (Ishii et al, 2019). Because the sliding velocity was ∼30% at 37 • C compared to the maximum, this previous finding suggests that thin filaments are partially activated in diastole at physiological body temperature, enabling rapid and efficient myocardial dynamics in systole (see Ishii et al, 2019 for details).…”

Section: Thermal Activation Of Thin Filamentsmentioning

confidence: 99%

“…The molecular mechanisms of thermal activation of thin filaments are yet to be fully understood. One possible mechanism is "partial dissociation" of Tn-Tm from F-actin upon increasing temperature (as discussed in Oyama et al, 2012;Ishii et al, 2019); viz., Tanaka and Oosawa (1971) demonstrated that Tm dissociates from F-actin at >∼40 • C. Later experiments by Ishiwata (1978) on reconstituted thin filaments (F-actin plus Tn-Tm) showed that Tn-Tm starts to partially dissociate from F-actin at ∼41 • C, with dissociation temperatures of 48.8 and 47.0 • C in the absence and presence of Ca 2+ , respectively.…”

Section: Thermal Activation Of Thin Filamentsmentioning

confidence: 99%

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Thermal Activation of Thin Filaments in Striated Muscle

et al. 2020

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In skeletal and cardiac muscles, contraction is triggered by an increase in the intracellular Ca 2+ concentration. During Ca 2+ transients, Ca 2+-binding to troponin C shifts the "on-off" equilibrium of the thin filament state toward the "on" sate, promoting actomyosin interaction. Likewise, recent studies have revealed that the thin filament state is under the influence of temperature; viz., an increase in temperature increases active force production. In this short review, we discuss the effects of temperature on the contractile performance of mammalian striated muscle at/around body temperature, focusing especially on the temperature-dependent shift of the "on-off" equilibrium of the thin filament state.

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Section: Thermal Activation Of Thin Filamentsmentioning

confidence: 99%

Section: Thermal Activation Of Thin Filamentsmentioning

confidence: 99%

Thermal Activation of Thin Filaments in Striated Muscle

et al. 2020

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“…In that study, the [Ca 2+ ] as measured by a calcium indicator (Fluo-4) was the same at 41 °C as in relaxed cells at 36 °C, suggesting that the contraction system of cardiomyocytes is activated at high temperatures even under conditions of very low [Ca 2+ ] 19 . This activation has also been confirmed in an in vitro motility assay using thin filaments reconstituted with α-tropomyosin and troponin 20 . These filaments were found to be able to slide at a rate of approximately 30% of full activity at a temperature of approximately 37 °C even at a low [Ca 2+ ] 20 .…”

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confidence: 54%

“…This activation has also been confirmed in an in vitro motility assay using thin filaments reconstituted with α-tropomyosin and troponin 20 . These filaments were found to be able to slide at a rate of approximately 30% of full activity at a temperature of approximately 37 °C even at a low [Ca 2+ ] 20 . Adult cardiomyocytes do not show SPOC with temperature jumps, but neonatal cardiomyocytes exhibit oscillatory contraction at high frequencies (6)(7)(8)(9)(10)(11)(12) under conditions of low-frequency Ca 2+ oscillation (~ 1.4 Hz) 18 .…”

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confidence: 54%

“…3e). The model used in this simulation is based on a previous model but newly assumes an effect of temperature 20 . A temperature increase from 37 to 41 °C was modeled by multiplying the rate constants of all the transitions by a factor of 7/4 and by multiplying the rate constant from the detachment (Det) state to the weak binding (WB) state by an additional factor of 2 ( K np in k +1 , Fig.…”

Section: Model Simulation Of Hsosmentioning

confidence: 99%

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Mechanism of contraction rhythm homeostasis for hyperthermal sarcomeric oscillations of neonatal cardiomyocytes

Shintani

Washio²,

Higuchi

2020

Sci Rep

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The heart rhythm is maintained by oscillatory changes in [Ca2+]. However, it has been suggested that the rapid drop in blood pressure that occurs with a slow decrease in [Ca2+] preceding early diastolic filling is related to the mechanism of rapid sarcomere lengthening associated with spontaneous tension oscillation at constant intermediate [Ca2+]. Here, we analyzed a new type of oscillation called hyperthermal sarcomeric oscillation. Sarcomeres in rat neonatal cardiomyocytes that were warmed at 38–42 °C oscillated at both slow (~ 1.4 Hz), Ca2+-dependent frequencies and fast (~ 7 Hz), Ca2+-independent frequencies. Our high-precision experimental observations revealed that the fast sarcomeric oscillation had high and low peak-to-peak amplitude at low and high [Ca2+], respectively; nevertheless, the oscillation period remained constant. Our numerical simulations suggest that the regular and fast rthythm is maintained by the unchanged cooperative binding behavior of myosin molecules during slow oscillatory changes in [Ca2+].

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Opto-thermal technologies for microscopic analysis of cellular temperature-sensing systems

2021

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Could enzymatic activities and their cooperative functions act as cellular temperature-sensing systems? This review introduces recent opto-thermal technologies for microscopic analyses of various types of cellular temperature-sensing system. Optical microheating technologies have been developed for local and rapid temperature manipulations at the cellular level. Advanced luminescent thermometers visualize the dynamics of cellular local temperature in space and time during microheating. An optical heater and thermometer can be combined into one smart nanomaterial that demonstrates hybrid function. These technologies have revealed a variety of cellular responses to spatial and temporal changes in temperature. Spatial temperature gradients cause asymmetric deformations during mitosis and neurite outgrowth. Rapid changes in temperature causes imbalance of intracellular Ca2+ homeostasis and membrane potential. Among those responses, heat-induced muscle contractions are highlighted. It is also demonstrated that the short-term heating hyperactivates molecular motors to exceed their maximal activities at optimal temperatures. We discuss future prospects for opto-thermal manipulation of cellular functions and contributions to obtain a deeper understanding of the mechanisms of cellular temperature-sensing systems.

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Microscopic heat pulses activate cardiac thin filaments

Cited by 15 publications

References 58 publications

Thermal Activation of Thin Filaments in Striated Muscle

Thermal Activation of Thin Filaments in Striated Muscle

Mechanism of contraction rhythm homeostasis for hyperthermal sarcomeric oscillations of neonatal cardiomyocytes

Opto-thermal technologies for microscopic analysis of cellular temperature-sensing systems

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