Microscopic histological characteristics of soft tissue sarcomas: analysis of tissue features and electrical resistance

Tosi, Anna Lisa; Campana, L.G.; Dughiero, Fabrizio; Forzan, Michele; Rastrelli, Marco; Sieni, Elisabetta; Rossi, Carlo Riccardo

doi:10.1007/s11517-016-1573-y

Cited by 13 publications

(8 citation statements)

References 49 publications

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“…Both HCC1954 and MDA-MB231 breast cancer cell lines were grown in a collagen-free scaffold based on crosslinked and lyophilized matrix components, such as hyaluronic acid and ionic-complementary self-assembling peptides (SAPs) condensed with the IKVAV (Ile-Lys-Val-Ala-Val) Laminin adhesion motif [45][46][47]. We previously demonstrated that it could reliably reproduce in vivo tissue architecture [48,49]. This scaffold result was particularly suitable to reproduce a tumor microenvironment where cells are embedded in a myxoid stroma, characterized by abundant amounts of hyaluronic acid and proteoglycans, low collagen, and elastin content [50].…”

Section: Introductionmentioning

confidence: 99%

The Efficiency of Gene Electrotransfer in Breast-Cancer Cell Lines Cultured on a Novel Collagen-Free 3D Scaffold

Sieni

Dettin

Robertis

et al. 2020

Cancers

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Gene Electro-Transfer (GET) is a powerful method of DNA delivery with great potential for medical applications. Although GET has been extensively studied in vitro and in vivo, the optimal parameters remain controversial. 2D cell cultures have been widely used to investigate GET protocols, but have intrinsic limitations, whereas 3D cultures may represent a more reliable model thanks to the capacity of reproducing the tumor architecture. Here we applied two GET protocols, using a plate or linear electrode, on 3D-cultured HCC1954 and MDA-MB231 breast cancer cell lines grown on a novel collagen-free 3D scaffold and compared results with conventional 2D cultures. To evaluate the electrotransfer efficiency, we used the plasmid pEGFP-C3 encoding the enhanced green fluorescent protein (EGFP) reporter gene. The novel 3D scaffold promoted extracellular matrix deposition, which particularly influences cell behavior in both in vitro cell cultures and in vivo tumor tissue. While the transfection efficiency was similar in the 2D-cultures, we observed significant differences in the 3D-model. The transfection efficiency in the 3D vs 2D model was 44% versus 15% (p < 0.01) and 24% versus 17% (p < 0.01) in HCC1954 and MDA-MB231 cell cultures, respectively. These findings suggest that the novel 3D scaffold allows reproducing, at least partially, the peculiar morphology of the original tumor tissues, thus allowing us to detect meaningful differences between the two cell lines. Following GET with plate electrodes, cell viability was higher in 3D-cultured HCC1954 (66%) and MDA-MB231 (96%) cell lines compared to their 2D counterpart (53% and 63%, respectively, p < 0.001). Based on these results, we propose the novel 3D scaffold as a reliable support for the preparation of cell cultures in GET studies. It may increase the reliability of in vitro assays and allow the optimization of GET parameters of in vivo protocols.

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Section: Introductionmentioning

confidence: 99%

The Efficiency of Gene Electrotransfer in Breast-Cancer Cell Lines Cultured on a Novel Collagen-Free 3D Scaffold

Sieni

Dettin

Robertis

et al. 2020

Cancers

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“…In this study, we present a new collagen-free 3D scaffold for cell culture aimed at reproducing as close as possible the tissue organization commonly found in tumors in vivo [30,31]. The new scaffold is a crosslinked and lyophilized matrix based on hyaluronic acid and ionic-complementary self-assembling peptides (SAPs) condensed with the IKVAV (Ile-Lys-Val-Ala-Val) Laminin adhesion motif [32,33,34].…”

Section: Introductionmentioning

confidence: 99%

A Novel 3D Scaffold for Cell Growth to Assess Electroporation Efficacy

Dettin

Sieni

Zamuner

et al. 2019

Cells

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Tumor electroporation (EP) refers to the permeabilization of the cell membrane by means of short electric pulses thus allowing the potentiation of chemotherapeutic drugs. Standard plate adhesion 2D cell cultures can simulate the in vivo environment only partially due to lack of cell–cell interaction and extracellular matrix (ECM). In this study, we assessed a novel 3D scaffold for cell cultures based on hyaluronic acid and ionic-complementary self-assembling peptides (SAPs), by studying the growth patterns of two different breast carcinoma cell lines (HCC1569 and MDA-MB231). This 3D scaffold modulates cell shape and induces extracellular matrix deposit around cells. In the MDA-MB 231 cell line, it allows three-dimensional growth of structures known as spheroids, while in HCC1569 it achieves a cell organization similar to that observed in vivo. Interestingly, we were able to visualize the electroporation effect on the cells seeded in the new scaffold by means of standard propidium iodide assay and fluorescence microscopy. Thanks to the presence of cell–cell and cell–ECM interactions, the new 3D scaffold may represent a more reliable support for EP studies than 2D cancer cell cultures and may be used to test new EP-delivered drugs and novel EP protocols.

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“…These experiments confirm the higher electric field intensity near the electrode when needles are more distant than 1 cm. Even if the simulation model did not consider the inhomogeneity of electrical characteristic of tissue due to the dependence on electric field intensity, 17 , 23 which is a characteristic of real tissue, 36 , 37 nevertheless, simulation results using a simple homogeneous model are useful to compare different electrodes in terms of distribution of electric field. The use of a more complex model for conductivity shows a higher electric field in the center model and comparable electric field near electrodes.…”

Section: Discussionmentioning

confidence: 99%

Effect of Electrode Distance in Grid Electrode: Numerical Models and In Vitro Tests

Ongaro

Campana

Mattei

et al. 2018

Technol Cancer Res Treat

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Electrochemotherapy is an emerging local treatment for the management of superficial tumors and, among these, also chest wall recurrences from breast cancer. Generally, the treatment of this peculiar type of tumor requires the coverage of large skin areas. In these cases, electrochemotherapy treatment by means of standard small size needle electrodes (an array of 0.73 cm spaced needles, which covers an area of 1.5 cm2) is time-consuming and can allow an inhomogeneous coverage of the target area. We have previously designed grid devices suitable for treating an area ranging from 12 to 200 cm2. In this study, we propose different approaches to study advantages and drawbacks of a grid device with needles positioned 2 cm apart. The described approach includes a numerical evaluation to estimate electric field intensity, followed by an experimental quantification of electroporation on a cell culture. The electric field generated in a conductive medium has been studied by means of 3-dimensional numerical models with varying needle pair distance from 1 to 2 cm. In particular, the electric field evaluation shows that the electric field intensity with varying needle distance is comparable in the area in the middle of the 2 electrodes. Differently, near needles, the electric field intensity increases with the increasing electrode distance and supply voltage. The computational results have been correlated with experimental ones obtained in vitro on cell culture. In particular, electroporation effect has been assessed on human breast cancer cell line MCF7, cultured in monolayer. The use of 2-cm distant needles, supplied by 2000 V, produced an electroporation effect in the whole area comprised between the electrodes. Areas of cell culture where reversible and irreversible electroporation occurred were identified under microscope by using fluorescent dyes. The coupling of computation and experimental results could be helpful to evaluate the effect of the needle distance on the electric field intensity in cell cultures in terms of reversible or irreversible electroporation.

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Microscopic histological characteristics of soft tissue sarcomas: analysis of tissue features and electrical resistance

Cited by 13 publications

References 49 publications

The Efficiency of Gene Electrotransfer in Breast-Cancer Cell Lines Cultured on a Novel Collagen-Free 3D Scaffold

The Efficiency of Gene Electrotransfer in Breast-Cancer Cell Lines Cultured on a Novel Collagen-Free 3D Scaffold

A Novel 3D Scaffold for Cell Growth to Assess Electroporation Efficacy

Effect of Electrode Distance in Grid Electrode: Numerical Models and In Vitro Tests

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