Microscopic, immunohistochemical and ultrastructural characterization of spontaneous lepidosarcomas in a neon tetra, Paracheirodon innesi, and a black crappie, Pomoxis nigromaculatus

Exploring Immunohistochemistry in Fish: Assessment of Antibody Reactivity by Western Immunoblotting

“…In mammals, vimentin is a common immunohistochemical marker for distinguishing between epithelial and mesenchymal tissues and identifying tumors exhibiting a mesenchymal phenotype, such as sarcomas [ 35 ]. In fish pathology, the V9 mouse monoclonal antibody is widely used for immunohistochemical analysis to identify normal and neoplastic mesenchymal cells with variable results [ 5 , 10 , 12 , 36 ]. The results of the present study show that the V9 clone does not effectively bind to its intended target protein in tested fish tissues, except for a non-specific WB-detectable band in rainbow trout.…”

“…Except for zebrafish ( Danio rerio ), researchers often rely on antibodies developed against human or mouse proteins, commonly lacking species-specificity. Despite their extensive use [ 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 ], mammalian antibodies must be optimized for aquatic animals [ 17 , 18 ]. Thus, false positives, negatives, and aberrant labelling are commonly described, despite manufacturers’ data sheets providing some information regarding expected cross-species reactivity.…”

Metastatic osteoblastic osteosarcoma of the jaw in a marble trout (Salmo marmoratus)

“…No metastases were observed in these cases except for a renal telangiectatic osteosarcoma (Rahmati-Holasoo et al, 2018). Finally, a particular osseous neoplasm deriving from dermal osteoblasts of scales, the lepidocytoma, has also been described (Stilwell et al, 2019).…”

Retrobulbar gangliocytoma and carcinoma of the corpuscles of Stannius in a pearl gourami, Trichopodus leerii (Bleeker, 1852)

“…Findings in this gourami expand the increasing number of neoplasms reported from ornamental fish, providing the first descriptions of a gangliocytoma, a rare ganglion cell tumour of the central nervous system, and a neoplasm of the corpuscles of Stannius, with features of a carcinoma, in a teleost fish. Along with gas gland adenomas and adenocarcinomas, swim bladder leiomyosarcomas, lepidocytomas and lepidosarcomas, branchioblastomas, pseudobranch adenomas and putative rodlet cell neoplasia, the corpuscle of Stannius carcinoma represents another example of neoplastic transformation in a unique anatomical structure or cell type of fish (Bowser et al., 2012; Brittelli, Chen, & Muska, 1985; Bunton & Wolfe, 1996; Stilwell, Boylan, Howard, & Camus, 2018; Stilwell, McManamon, Sturgeon, Camus, & Camus, 2019; Wellings, Alpers, McCain, & Myers, 1977; Wolf, Smith, Jortner, & McMaster, 2018).…”