Microscopic Study of Bovine Serum Albumin Adsorption on Zinc Oxide (0001) Surface

Rezek, Bohuslav; Hematian, Hadi; Jíra, Jaroslav; Rutherford, David; Kuliček, Jaroslav; Ukraintsev, Egor; Remeš, Z.

doi:10.1002/pssa.202000558

Cited by 5 publications

(9 citation statements)

References 36 publications

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“…To analyze not only the topography but also the adhesion of the BSA molecules, we employed nanoshaving experiments. They have been previously well proven in studies of DNA molecules grafted on diamond, [46] BSA molecules adsorbed on plasma polymer coating, [47] on diamond [48] and on ZnO

(0001)

surface [39] . Hereby, we perform them on the BSA to analyze the layer thickness and adhesion properties of BSA layers on various ZnO surfaces.…”

Section: Methodsmentioning

confidence: 98%

“…FF calculations were performed to determine the most likely orientation of BSA over different ZnO surfaces. We used same FF methodology as in our recently published papers [39,40] . Briefly, the systems under the study consist of BSA molecules and ZnO slabs that are available from the RCSB Protein Data Bank (pdb ID of 4F5S) and QuantumATK software database respectively.…”

Section: Methodsmentioning

confidence: 99%

“…Interactions of 7.5 nm ZnO nanoparticles with BSA were investigated at different temperatures, indicating a decrease in binding sites with the increase of temperature [38] . Our recent studies theoretically examined the different orientations of the BSA on two ZnO non‐polar and Zn‐faced polar surfaces [39,40] . A more detailed inspection of the FF calculation results in these studies reveals that the interactions between BSA and ZnO surfaces in different orientations are mainly about the interactions between specific amino acid residues (ASP, GLU, GLN, LYS, and SER displayed in Figure 1b) with the atoms on ZnO surfaces.…”

Section: Introductionmentioning

confidence: 99%

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Strong Structural and Electronic Binding of Bovine Serum Albumin to ZnO via Specific Amino Acid Residues and Zinc Atoms

2021

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ZnO biointerfaces with serum albumin have attracted noticeable attention due to the increasing interest in developing ZnO‐based materials for biomedical applications. ZnO surface morphology and chemistry are expected to play a critical role on the structural, optical, and electronic properties of albumin‐ZnO complexes. Yet there are still large gaps in the understanding of these biological interfaces. Herein we comprehensively elucidate the interactions at such interfaces by using atomic force microscopy and nanoshaving experiments to determine roughness, thickness, and adhesion properties of BSA layers adsorbed on the most typical polar and non‐polar ZnO single‐crystal facets. These experiments are corroborated by force field (FF) and density‐functional tight‐binding (DFTB) calculations on ZnO‐BSA interfaces. We show that BSA adsorbs on all the studied ZnO surfaces while interactions of BSA with ZnO are found to be considerably affected by the atomic surface structure of ZnO. BSA layers on the (0001‾) surface have the highest roughness and thickness, hinting at a specific upright BSA arrangement. BSA layers on (101‾0) surface have the strongest binding, which is well correlated with DFTB simulations showing atomic rearrangement and bonding between specific amino acids (AAs) and ZnO. Besides the structural properties, the ZnO interaction with these AAs also controls the charge transfer and HOMO‐LUMO energy positions in the BSA‐ZnO complexes. This ZnO facet‐specific protein binding and related structural and electronic effects can be useful for improving the design and functionality of ZnO‐based materials and devices.

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(0001)

surface [39] . Hereby, we perform them on the BSA to analyze the layer thickness and adhesion properties of BSA layers on various ZnO surfaces.…”

Section: Methodsmentioning

confidence: 98%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Strong Structural and Electronic Binding of Bovine Serum Albumin to ZnO via Specific Amino Acid Residues and Zinc Atoms

2021

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“…The AFM observations were corroborated by atomic-scale simulations of BSA on the (0001) ZnO surface using the force-field method and showing rearrangements of Zn surface atoms. 31 …”

Section: Resultsmentioning

confidence: 99%

Preparation of Barbed ZnO Fibers and the Selective Adsorption Behavior for BSA

Liu

Dai

2021

ACS Omega

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ZnO electrospun nanofibers can act as seed fibers to fabricate multidentate barbed fibers perpendicular to the growth of the fibers using the chemical bath deposition (CBD) method. Fibers with a multirod morphology have a porous grid structure. The sample is easy to recover, and the nonpolar surface in the sample is sufficiently exposed. In the research of barbed fiber fabrication and adsorption on bovine serum albumin (BSA), the effects of different chemical bath conditions on the growth of ZnO nanorods were discussed. Barbed fibers with large slenderness ratios were obtained at a water content of 60 mL at 75 °C. Each milligram of barbed fibers can quickly adsorb about 162 μg of protein within 30 min. The adsorption activity of BSA between polar and nonpolar ZnO surfaces was also studied. The selective adsorption behavior of BSA on the nonpolar surface was revealed.

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“…Thus, their behavior in the colloid as well as inherent properties can be suppressed or enhanced by surface interaction with other molecules that are also present in the liquid such as proteins or carbohydrates. Our prior computer simulations showed that the interaction between amino acids and zinc oxide surface involved covalent bonding and not merely due to passive adsorption [8].…”

Section: Introductionmentioning

confidence: 97%

Plasma-synthesised Zinc oxide nanoparticle behavior in liquids

Rutherford

Jíra

Kolářová

et al. 2021

NANOCON 2021 Conference Proeedings

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Zinc oxide nanoparticles have been synthesized using non-thermal atmospheric pressure plasma (ZnO-NTP). We investigated the behavior of these ligand-free as a colloid suspension using different solvents, from deionized water to physiological saline and microbial culture broth. We found that the zeta potential of ZnO-NTP became more negative after exposure to microbial culture broth relative to water, which suggests increased colloid stability. Photoluminescence spectra of ZnO-NTP were similar regardless of liquid type, yet optical and fluorescent images of samples showed different agglomeration behavior depending on liquid type. Scanning electron microscopy images revealed large agglomerates of ZnO-NTP interacting with the surface of bacteria cells, ranging in size from 200 nm up to 2 µm. We also studied effect of sub-lethal concentrations of ZnO-NTP on bacteria under illumination. There was no significant difference in viable bacteria concentration after 24h exposure to 10 µg/mL ZnO-NTP relative to untreated control irrespective of sample illumination.

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Microscopic Study of Bovine Serum Albumin Adsorption on Zinc Oxide (0001) Surface

Cited by 5 publications

References 36 publications

Strong Structural and Electronic Binding of Bovine Serum Albumin to ZnO via Specific Amino Acid Residues and Zinc Atoms

Strong Structural and Electronic Binding of Bovine Serum Albumin to ZnO via Specific Amino Acid Residues and Zinc Atoms

Preparation of Barbed ZnO Fibers and the Selective Adsorption Behavior for BSA

Plasma-synthesised Zinc oxide nanoparticle behavior in liquids

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