Microscopy imaging of liposomes: From coverslips to environmental SEM

Bibi, Sagida; Kaur, Randip; Henriksen‐Lacey, Malou; McNeil, Sarah E.; Wilkhu, Jitinder; Lattmann, Eric; Christensen, Dennis; Mohammed, Afzal-Ur-Rahman; Perrie, Yvonne

doi:10.1016/j.ijpharm.2010.12.021

Cited by 119 publications

(103 citation statements)

References 31 publications

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“…A review of the literature indicates that microscopic methods are a valuable tool for the evaluation of various drug forms, including the parenteral forms created with the use (20)(21)(22)(23)(24) Att ribute of phospholipids. The simplest method, frequently used for initial testing, is optical light microscopy and its various modifi cations, while electron microscopy and atomic force microscopy are used when more detailed information is required (20)(21)(22).…”

Section: Microscopic Techniques Fo R Visualization Of Phospholipid Drmentioning

confidence: 99%

“…The simplest method, frequently used for initial testing, is optical light microscopy and its various modifi cations, while electron microscopy and atomic force microscopy are used when more detailed information is required (20)(21)(22). Each of these methods requires sample preparation in a specifi c way, depending on the degree of complexity, and each involves diff erent capabilities and certain limitations, e.g., the risk of error or artifact formation.…”

Section: Microscopic Techniques Fo R Visualization Of Phospholipid Drmentioning

confidence: 99%

“…However, at best, the resolution of light microscopes is not higher than 0.2 m. Due to method limitations (small magnifi cation and low resolution), the light microscope is used only during the initial stages of testing conducted on submicron systems (22). An indisputable benefi t of this method, however, is the apparatus availability, simple and user-friendly use of the microscope and fast preparation of the analyzed samples.…”

Section: Microscopic Techniques Fo R Visualization Of Phospholipid Drmentioning

confidence: 99%

“…3). Their anisotropic properties result from the ordered, concentric arrangement of phospholipid layers (22,28,29). Once the polarized rays pass through the lamellar layers, arranged perpendicularly to the polarization plane, the so called "maltese cross" textures appear in the microscopic image.…”

Section: Microscopic Techniques Fo R Visualization Of Phospholipid Drmentioning

confidence: 99%

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Microscopic methods in analysis of submicron phospholipid dispersions

Płaczek

Kosela

2016

Acta Pharmaceutica

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Microscopy belongs to the group of tests, used in pharmaceutical technology, that despite the lapse of time and the development of new analytical methods, still remain irreplaceable for the characterization of dispersed drug dosage forms (e.g., suspensions and emulsions). To obtain complete description of a specific drug formulation, such as parenteral colloidal products, a combination of different microscopic techniques is sometimes required. Electron microscopy methods are the most useful ones; however, even such basic methods as optical microscopy may be helpful for determination of some properties of a sample. The publication explicates the most popular microscopical techniques used nowadays for characterization of the morphology of nanoparticles suspended in pharmaceutical formulations; ad vantages and disadvantages of these methods are also discussed. Parenteral submicron formulations containing lecithin or a particular phospholipid were chosen as examples.

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Section: Microscopic Techniques Fo R Visualization Of Phospholipid Drmentioning

confidence: 99%

Section: Microscopic Techniques Fo R Visualization Of Phospholipid Drmentioning

confidence: 99%

Section: Microscopic Techniques Fo R Visualization Of Phospholipid Drmentioning

confidence: 99%

Section: Microscopic Techniques Fo R Visualization Of Phospholipid Drmentioning

confidence: 99%

See 2 more Smart Citations

Microscopic methods in analysis of submicron phospholipid dispersions

Płaczek

Kosela

2016

Acta Pharmaceutica

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“…Additionally, AFM has been utilized to show liposome morphology (especially for small liposomes), size and stability without sample manipulation. AFM analysis is a rapid and powerful technique to obtain information about liposome morphology and aggregation processes during their storage [226,[237][238][239].…”

Section: Morphology Of Liposomesmentioning

confidence: 99%