Microscopy

Sprott, G. Dennis; Beveridge, Terry J.

doi:10.1007/978-1-4615-2391-8_3

Cited by 16 publications

(10 citation statements)

References 107 publications

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“…1c, e, f). It has been suggested that these tubular structures connect the S-layer to the cytoplasmic membrane (Sprott & Beveridge, 1994) and they are identical in appearance to those observed in Methanosarcina mazei (Robinson, 1986). Additionally, in many cells, an unknown structure was observed in the cytoplasm (US in Fig.…”

Section: Cells Of E1-9csupporting

confidence: 54%

“…T is different from a single S-layer and that this thick layer may play a role in adaptation to low-ionic-strength environments, analogous to the methanochondroitin layer in Methanosarcina species (Sprott & Beveridge, 1994). In thin sections, the cytoplasm of some cells apparently shrank away from the outer layer as a result of the osmotic strength of the 2 % glutaraldehyde fixative, and tubular structures were visible in the intervening space (T in Fig.…”

Section: Cells Of E1-9cmentioning

confidence: 98%

“…1c, d, f) resembling arrays of protein complexes,~10 nm in diameter, that were less electron-dense than ribosomes. These structures do not resemble the 'methanochondrion' internal membrane systems, now believed to be preparation artefacts (Sprott & Beveridge, 1994). The potential protein complexes are similar in size to purified methylcoenzyme M methylreductase complexes (~10 nm) and smaller than F 420 -reducing hydrogenase complexes (~15 nm) (Wackett et al, 1987).…”

Section: Cells Of E1-9cmentioning

confidence: 99%

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Methanosphaerula palustris gen. nov., sp. nov., a hydrogenotrophic methanogen isolated from a minerotrophic fen peatland

Cadillo‐Quiroz

Yavitt

Zinder

2009

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

106

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Peat-forming wetlands or peatlands (Gore, 1983) store nearly 30 % of all terrestrial carbon (Gorham, 1991) and are estimated to contribute around 20 % (115 Tg) of total annual CH 4 emissions to the atmosphere (Cicerone & Oremland, 1988). Hence, the methanogenic archaea inhabiting these ecosystems are of importance, particularly since the CH 4 concentration in the atmosphere has more than doubled in the past 200 years (Cicerone & Oremland, 1988). Surveys of 16S rRNA gene sequences from acidic and neutral pH peatlands have shown a broad diversity of euryarchaeal sequences (Cadillo-Quiroz et al., 2006Galand et al., 2003;Kotsyurbenko et al., 2004). The majority of sequences are not closely related to cultured strains, but instead form independent clusters, suggesting the existence of novel euryarchaeal groups. Two of these clusters, named groups E1 and E2 in the , 2006Galand et al., 2003; Hales et al., 1996). The order Methanomicrobiales is diverse and its members exhibit great morphological and physiological variability (Dubach & Bachofen, 1985). This order comprises the families Methanomicrobiaceae, Methanocorpusculaceae and Methanospirillaceae (Boone et al., 2001;Garcia et al., 2000). It also includes three genera that are not placed within any family: the genus Methanocalculus, which is listed as a genus incertae sedis (Boone et al., 2001), with four reported species (Lai et al., 2002Mori et al., 2000;Ollivier et al., 1998), the proposed genus 'Candidatus Methanoregula', with one species, 'Candidatus Methanoregula boonei', isolated from an acidic bog (Bräuer et al., 2006a, b), and the recently described genus Methanolinea, with one species, Methanolinea tarda, isolated from sewage sludge (Imachi et al., 2008).Abbreviations: TEM, transmission electron microscopy; T-RFLP, terminal restriction fragment length polymorphism.The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA and mcrA gene sequences of strain E1-9c T are EU156000 and EU296536, respectively.Plots showing the effects of sodium ion concentration on growth of strain E1-9c T and formate utilization by strain E1-9c T are available as supplementary material with the online version of this paper.

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Section: Cells Of E1-9csupporting

confidence: 54%

Section: Cells Of E1-9cmentioning

confidence: 98%

Section: Cells Of E1-9cmentioning

confidence: 99%

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Methanosphaerula palustris gen. nov., sp. nov., a hydrogenotrophic methanogen isolated from a minerotrophic fen peatland

Cadillo‐Quiroz

Yavitt

Zinder

2009

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

106

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“…This expectation is strengthened by the finding that tetraether lipid-rich Thermoplasma membranes do not exhibit a fracture plane (16). However, it is difficult to interpret much of the past freeze-fracture data on methanogen cells in this context, since the frequency of intramembrane fractures compared with cross fractures is rarely reported (21).…”

mentioning

confidence: 99%

Freeze-fracture planes of methanogen membranes correlate with the content of tetraether lipids

et al. 1993

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Methanospirillum hungatei GP1 contained 50%1 of its ether core lipids (polar lipids less head groups) as tetraether lipids, and its plasma membrane failed to fracture along its hydrophobic domain during freeze-etching. The membrane ofMethanosaeta ("Methanothrir") conciii did not contain tetraether lipids and easily fractured to reveal typical intramembranous particles. Methanococcus jannaschii grown at 500C contained 20%k tetraether core lipids, which increased to 45% when cells were grown at 70'C. The frequency of membrane fracture was reduced as the membrane-spanning tetraether lipids approached 45%. As the tetraether lipid content increased, and while fracture was still possible, the particle density in the membrane increased; these added particles could be tetraether lipid complexes torn from the opposing membrane face. The diether membrane (no tetraether lipid) of Methanococcus volkae easily fractured, and the intramembranous particle density was low. Protein-free liposomes containing tetraether core lipids (ca. 45%) also did not fracture, whereas those made up exclusively of diether lipids did split, indicating that tetraether lipids add considerable vertical stability to the membrane. At tetraether lipid concentrations below 45%, liposome bilayers fractured to reveal small intramembranous particles which we interpret to be tetraether lipid complexes.

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“…No other member of the domain Archaea has yet been described that will show the variety of uni-and multicellular forms displayed by M. mazei (Sprott & Beveridge, 1993). This organism, therefore, affords a unique archaeal model suitable to study development of complex multicellular structures in response to environmental changes that might have played a role in evolution, and in response to stressors occurring today in nature and in the laboratory.…”

Section: Introductionmentioning

confidence: 95%

Integration of Foreign DNA in an Intergenic Region of the ArchaeonMethanosarcina mazeiWithout Effect on Transcription of Adjacent Genes

Macario

Guerrini

Dugan

et al. 1996

Journal of Molecular Biology

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Microscopy

Cited by 16 publications

References 107 publications

Methanosphaerula palustris gen. nov., sp. nov., a hydrogenotrophic methanogen isolated from a minerotrophic fen peatland

Methanosphaerula palustris gen. nov., sp. nov., a hydrogenotrophic methanogen isolated from a minerotrophic fen peatland

Freeze-fracture planes of methanogen membranes correlate with the content of tetraether lipids

Integration of Foreign DNA in an Intergenic Region of the ArchaeonMethanosarcina mazeiWithout Effect on Transcription of Adjacent Genes

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