Microsecond-Pulsed Dielectric Barrier Discharge Plasma-Treated Mist for Inactivation of Escherichia coli &lt;italic&gt;In Vitro&lt;/italic&gt;

One of the major concerns in the COVID-19 pandemic is related to the possible transmission in poorly ventilated spaces of SARS-CoV-2 through aerosol microdroplets, which can remain in the air for long periods of time and be transmitted to others over distances >1 m. Cold atmospheric pressure plasmas can represent a promising solution, thanks to their ability in producing a blend of many reactive species, which can inactivate the airborne aerosolized microorganisms. In this study, a dielectric barrier discharge plasma source is used to directly inactivate suitably produced bioaerosols containing Staphylococcus epidermidis or purified SARS-CoV-2 RNA flowing through it. Results show that for low residence times (<0.2 s) in the plasma region a 3.7 log R on bacterial bioaerosol and degradation of viral RNA can be achieved. K E Y W O R D S bioaerosol, cold plasma, inactivation, indoor airborne transmission, SARS-CoV-2 Alina Bisag and Pasquale Isabelli contributed equally to this study.

show abstract

“…In this way, plasma can simultaneously act as a particle filter as well as a disinfection technology. [ 23,26,27 ]…”

Section: Introductionmentioning

confidence: 99%

Cold atmospheric plasma inactivation of aerosolized microdroplets containing bacteria and purified SARS‐CoV‐2 RNA to contrast airborne indoor transmission

Bisag

Isabelli

Laurita

et al. 2020

Plasma Processes & Polymers

View full text Add to dashboard Cite

show abstract

“…All these recent advances should, in our opinion and as has already been documented in the literature [19,20,22], result in a strong effort and a specific interest in the development, optimization and applications of plasma in interaction with liquid droplets and aerosols in various fields of technology over the next decade. The topic of this paper is to summarize the opportunities for generating and delivering plasmas into aerosols and sprays, as a specific branch of plasma-liquid science and technology.…”

Section: Plasma and Aerosols: Opportunities Economical And Societal mentioning

confidence: 76%

“…The most common experimental setups, involving the direct contact of a narrow gas discharge with a batch solution, limit the production of reactive species and, thus, process throughput. While direct contact with a flowing liquid film has been proposed as an alternative to increase the plasma-liquid interface [17,18], some recent works have proposed the use of plasma-droplet interactions with the aim of overcoming the limit of batch process enabling in in-situ and on-demand dispensing [19][20][21]. The large number of reaction pathways and available plasma parameters, already challenging for batch processes, bring even more complexity to the analysis and investigation of plasma-liquid interaction but open new possibilities for technological applications.…”

Section: Plasma-liquid As a Bench Of Emerging Applications From 2010mentioning

confidence: 99%

“…Concerning droplets modifications due to the interaction with plasma, a droplet entering a plasma region is subjected to charging and subsequently experiencing deformation and possibly splitting due to Coulombic fission as a result of exposure to a plasma-related electric field [20]. In addition, the rate of evaporation of such droplets could be enhanced by additional factors other than those encountered in non-reactive gases, such as irradiation by ultra-low energy electrons [34].…”

mentioning

confidence: 99%

See 1 more Smart Citation

Plasma and Aerosols: Challenges, Opportunities and Perspectives

et al. 2019

View full text Add to dashboard Cite

The interaction of plasmas and liquid aerosols offers special advantages and opens new perspectives for plasma–liquid applications. The paper focuses on the key research challenges and potential of plasma-aerosol interaction at atmospheric pressure in several fields, outlining opportunities and benefits in terms of process tuning and throughputs. After a short overview of the recent achievements in plasma–liquid field, the possible application benefits from aerosol injection in combination with plasma discharge are listed and discussed. Since the nature of the chemicophysical plasma-droplet interactions is still unclear, a multidisciplinary approach is recommended to overcome the current lack of knowledge and to open the plasma communities to scientists from other fields, already active in biphasic systems diagnostic. In this perspective, a better understanding of the high chemical reactivity of gas–liquid reactions will bring new opportunities for plasma assisted in-situ and on-demand reactive species production and material processing.

show abstract

“…Electrostatic spray requires a closed circuit with the target side grounded and a capillary to which a high voltage is applied, making it unsafe for biological applications. On the other hand, a dielectric barrier discharge with the closed circuit can be used to impart sterilization and other effects derived from reactive chemical species generated by the plasma 17 . However, it is difficult to generate liquid particles only by the dielectric barrier discharge, which requires another mist generation mechanism such as an ultrasonic transducer.…”

Section: Introductionmentioning

confidence: 99%

Potential generation of nano-sized mist by passing a solution through dielectric barrier discharge

Watanabe

Tanaka

Miyaji

et al. 2022

Sci Rep

View full text Add to dashboard Cite

Plasma medicine, a therapeutic technology that uses atmospheric-pressure plasma, is attracting much attention as an innovative tool for the medical field. Most of the plasma biomedical tools use direct effects, such as heat, optical stimulation, and reactive chemical species, on the lesion. Nanoparticulation techniques using indirect action by plasma, i.e., generation of electric fields, have the potential to be applied to promote transdermal absorption, where drugs pass through the barrier function of skin and penetrate into internal tissues. Here, we show a method to directly generate the nano-sized mist by passing a solution through the dielectric barrier discharge. This method enables us to produce the mist potentially in the nanometer size range for both water-based and oil-based solutions. Ease of mist generation was influenced by the plasma-induced changes in physical and chemical characteristics, including electrical conductivity, viscosity, and chemical species. We anticipate the developed method for nano-sized mist generation to provide a technique in the applications of the transdermal absorption system, including those related to pharmaceuticals and cosmetics.

show abstract

Microsecond-Pulsed Dielectric Barrier Discharge Plasma-Treated Mist for Inactivation of Escherichia coli <italic>In Vitro</italic>

Cited by 16 publications

References 52 publications

Cold atmospheric plasma inactivation of aerosolized microdroplets containing bacteria and purified SARS‐CoV‐2 RNA to contrast airborne indoor transmission

Cold atmospheric plasma inactivation of aerosolized microdroplets containing bacteria and purified SARS‐CoV‐2 RNA to contrast airborne indoor transmission

Plasma and Aerosols: Challenges, Opportunities and Perspectives

Potential generation of nano-sized mist by passing a solution through dielectric barrier discharge

Contact Info

Product

Resources

About