Microshell Arrays Enhanced Sensitivity in Detection of Specific Antibody for Reduced Graphene Oxide Optical Sensor

Abstract

Protein-protein interactions play an important role in the investigation of biomolecules. In this paper, we reported on the use of a reduced graphene oxide microshell (RGOM)-based optical biosensor for the determination of goat anti-rabbit IgG. The biosensor was prepared through a self-assembly of monolayers of monodisperse polystyrene microspheres, combined with a high-temperature reduction, in order to decorate the RGOM with rabbit IgG. The periodic microshells allowed a simpler functionalization and modification of RGOM with bioreceptor units, than reduced graphene oxide (RGO). With additional antibody-antigen binding, the RGOM-based biosensor achieved better real-time and label-free detection. The RGOM-based biosensor presented a more satisfactory response to goat anti-rabbit IgG than the RGO-based biosensor. This method is promising for immobilizing biomolecules on graphene surfaces and for the fabrication of biosensors with enhanced sensitivity.

Graphene and its Derivatives-Based Optical Sensors

“…(E) Signal changes caused by the interaction of antigen and antibody. (F) Optical microscopy and SEM image of reduced graphene oxide microshell (RGOM) (Jiang et al, 2017a). (G) Schematic diagram of the photothermal detection experimental setup (Gao et al, 2018b).…”

“…The graphene based optical sensor shows a satisfactory response to rabbit IgG with a minimum concentration of 0.0625 μg/ml. Furthermore, Jiang et al developed a reduced graphene oxide microshell (RGOM)based optical biosensor for the determination of goat antirabbit IgG (Jiang et al, 2017a). In Figure 5F, the RGOM was prepared through a self-assembly of monolayer of monodisperse polystyrene microspheres.…”

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“…Then, they were rinsed with deionized water and dried with nitrogen. A piranha solution was used to make the substrates highly hydrophilic and easily bond with PS spheres by immersing SiO 2 in the solution at 80°C for 1 h. A monolayer hexagonal close-packed array of monodispersed PS spheres with diameters from 200 to 750 nm were formed on a SiO 2 surface using a self-assembly process 19 by vertically inserting the clean substrates into various PS solution with different PS sphere sizes.…”