Microspectrophotometric Cell Analysis

Thiessen, G.; Thiessen, Hella

doi:10.1016/s0079-6336(77)80006-5

Cited by 19 publications

(3 citation statements)

References 185 publications

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“…Consequently, some of the grains located either above or below the foeal plane appear to be lighter than those grains loeated in the foeal plane. These apparent differences in gray values made successful application of the customary cytophotometrie methods (7,16) :> :j <:…”

Section: Resultsmentioning

confidence: 99%

Automated quantitative analysis of single and double label autoradiographs.

Rüter¹,

Aus²,

Harms³

et al. 1979

J Histochem Cytochem.

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A method for the analysis of silver grain content in both single and double label autoradiographs is presented. The total grain area is calculated by counting the number of pixels at which the recorded light intensity in transmission dark field illumination exceeds a selected threshold. The calibration tests included autoradiographs with low (3H-thymidin) and high eH-de80xyuridin) silver grain density. The results are proportional to the customary visual grain count. For the range of visibly countable grain densities in single labeled specimens, the correlation coefficient between the computed values and the visual grain counts is better than 0.96. In the first emulsion of the two emulsion layer autoradiographs of double labeled specimens eH-14 C-thymidin) the correlation coefficient is 0.919 and 0.906. The method provides a statistical correction for the background grains not due to the isotope. The possibility to record I·C tracks by shifting the focus through the second emulsion of the double labeled specimens is also demonstrated. The reported technique is essentially independent of size, shape and density of the grains.The visual evaluation of autoradiographs in the light microscope is a common and important technique in both experimental and clinical cytology. The grains observed in the photographic emulsions covering the biologic specimen reveal the location and amount of the incorporated radioactive label. Both single labeled and double labeled specimens can be used. In the former, only one isotope CH) and one thin emulsion layer are used. In the latter, two isotopes CH, I·C) with different ß-energies and two separate emulsion layers are used. The first thin layer covers the cells directly and serves to register the low energy "H electrons. The second, thick emulsion permits the registration of the high energy 14C electrons. To prevent "H electrons from entering the second emulsion, the two emulsions are separated from each other by a neutral layer of gelatin. The size, shape, number and location of the visibly countable grains varles to a certain extent with the preparation technique, the exposure time, the ftlm developer and the type of photographic emulsion. In visual, nonautomated evaluation of the autoradiographs, the investigator counts the visible clusters of silver ftlament. Especially at high grain densities, the dissection of these clusters into individual grains of similar size and shape is frequently impossible. The evaluation of all autoradiographs is further complicated by the presence of background grains in the emulsion that are not due to the charged particle flux from the isotope label. Therefore, an integrated silver grain area count, statistically corrected to minimize the effect of the background grain distribution, may be more closely correlated to the charged particle flux than the customary visual count (9). Using routine autoradiographs, the results presented in I Supported in part by the Deutsche Forschungsgemeinschaft, Sonderforschungsbereich 105 Würzburg and by the Bundesminis...

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Section: Resultsmentioning

confidence: 99%

Automated quantitative analysis of single and double label autoradiographs.

Rüter¹,

Aus²,

Harms³

et al. 1979

J Histochem Cytochem.

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“…[41,52] Additionally, the cytotoxicity of the prepared complexes was investigated by MTT assay utilizing an MTT assay kit (Sigma, USA). [53]…”

Section: Theoretical Calculationsmentioning

confidence: 99%

Synthesis, structural characterization, density functional theory calculations, and antimicrobial, anticancer, and antimetastatic properties of nanosized heteroleptic complexes of cocaine/TMEDA with d‐block metal ions

Abdallah

Zaki

Mahmoud

et al. 2021

Applied Organom Chemis

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In the field of transition metal chemistry, the development of transition metal‐based drugs for the treatment of diseases such as cancer or microbial infections with minimization of adverse effects and drug resistance constitutes an active area of research. Herein, eight novel nanosized heteroleptic complexes of cocaine/TMEDA with the formula [M(COC)(TMEDA)Cly(OH2)z]nCl·xH2O (M = Cr(III), Mn(II), Fe(III), Co(II), Ni(II), Cu(II), Zn(II), and Cd(II); COC = cocaine; TMEDA = N,N,N′,N′‐tetramethylethylenediamine, y = 1–2; z = 0–1; n = 0–1; and x = 0–2) were synthesized and structurally characterized via elemental analysis, molar conductivity, mass spectrometry, and spectroscopic and microscopic techniques. The thermal properties and kinetic thermodynamic parameters of the synthesized complexes were also studied. The geometry and electronic structures were investigated via density functional theory (DFT) calculations. The antiproliferative activity of the complexes on HepG‐2 and MCF‐7 cancer cell lines was quantified via MTT assay. The Fe(III) and Cd(II) complexes exhibited promising cytotoxic activities against the HepG‐2 and MCF‐7 cancer cell lines, respectively, with minimum effect on HFB4 human normal cells. Further molecular mechanistic studies were performed on the Cd(II) complex to inspect its influence on different cancer pathophysiology‐related processes in the MCF‐7 cell line including metastasis, apoptosis, and cellular oxidative stress and on the cellular levels of the human tumor suppressor nuclear proteins p21 and p27. The results revealed that the Cd(II) complex is a promising anticancer agent that acts through several molecular mechanisms with minimum effect on the normal cells and with additional antimetastatic properties. Furthermore, the antibacterial and antifungal activities of the prepared complexes were investigated.

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“…Biology and Medicine. See Books (13, 28, 122,122a, 247, 256,365); Instruments, Microscopes (27,187); Instruments, Stages (82); Polarized Light Microscopy (1,32,60,113,114,249,(318)(319)(320)(321)330), Microphotometry and Microspectrophotometry (124,329); Interference Microscopy (155); Fluorescence Microscopy (342,372); Dark Field and Ultramicroscopy (232); Ultraviolet and Infrared Microscopy (40); Techniques of Specimen Preparation, Miscellaneous (154); Heating and Fusion Methods (193); Stereology (19,135,197, 218. 352, 355, 361); Automatic Image Analysis (10,84,163,350); and Techniques for Specimen Examination (285).…”

Section: Applications Of Chemical Microscopymentioning

confidence: 99%

Chemical microscopy

Cocks¹

1978

Anal. Chem.

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Microspectrophotometric Cell Analysis

Cited by 19 publications

References 185 publications

Automated quantitative analysis of single and double label autoradiographs.

Automated quantitative analysis of single and double label autoradiographs.

Synthesis, structural characterization, density functional theory calculations, and antimicrobial, anticancer, and antimetastatic properties of nanosized heteroleptic complexes of cocaine/TMEDA with d‐block metal ions

Chemical microscopy

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