MICROSPECTROPHOTOMETRIC STUDIES ON THE PIGMENTSIN VIVOOF SINGLE ALGAL CELLS‐I. PIGMENTS OFCHLORELLA PYRENOIDOSA

Abstract

Abstract— The pigments in vivo of single cells of Chlorella pyrenoidosa were studied by the microspectrophotometric technique. An accessory recording the first derivative of absorption was used to obtain fine resolution and enhanced accuracy. The results suggest that there are several long‐wavelength components of Chl a in vivo. In addition, there seem to be four short‐wave forms of Chl a. It is also likely that Chl b exists in vivo in two different forms. The existence of all these forms was demonstrated at room temperature.

Detailed Action Spectra of Photophosphorylation Catalyzed by Photosystem I

“…Fluorescence spectra and derivative absorption spectra provide evidence for the occurrence of an absorption band at 720 nm cf. GOEDHEER (1972) andTHOMAS et al (1970). The only action spectra we know that show an absorption band around 720 nm are the ones mentioned by LITVIN & SINESHCHEKOV (1975) and those published by VAN GINKEL (1975) andHALLDAL et al (1974).…”

“…Derivative spectroscopy suggests the occurrence of chlorophyll-a forms with absorption peaks at 662, 669, 672 and 678 nm in this spectral region, cf. THOMAS et al (1970). However, fourth derivative low temperature absorption spectra, deconvoluted low temperature absorption spectra and deconvoluted photophosphorylation action spectra suggest the occurrence of only four chlorophyll absorption bands peaking at 661 (662), 669 (670), 677 and 684 nm, cf.…”

“…In the spectral region ranging from 690-730 nm derivative spectroscopy suggests the occurrence of chlorophyll a forms with absorption peaks around 725-730, 716, 709-712, 702-705, 696 and690-693 nm, cf. GULYAYEV &LITVIN (1970) andTHOMAS et al (1970). A number of absorption spectra and action spectra show the presence of an absorption band at 700-705 nm cf.…”

Vidyadhar Govind (Pandit) Tatake (1926–2004): An ingenious instrumentalist, an authority on thermoluminescence, and a lover of classical Indian music

“…He employed these to learn more and in detail about effects of ionizing and non-ionizing radiations on biological materials. Together with Dr. A. R. Gopal-Ayengar, a pioneer in biological sciences in India at that time, Tatake used microspectrophotometry to identify different pigments of the green alga Chlorella (Thomas et al 1970a) and multiple forms of chlorophyll a in the green algae Chlamydomonas reinhardtii and Scenedesmus obliquus (Thomas et al 1970b). These were important papers since C. Stacy French, at the Carnegie Institution of Washington at Stanford, was investigating these multiple spectral forms of chlorophyll a mainly in suspensions of algae (see e.g., French 1971; Govindjee and Fork 2006).…”