Microspheres in bone regeneration: Fabrication, properties and applications

Cai, Zhuyun; Jiang, Heng; Lin, Tong; Ma, Jun; Gao, Rui; Jiang, Yingying; Zhou, Guiyao

doi:10.1016/j.mtadv.2022.100315

Cited by 18 publications

(34 citation statements)

References 149 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Macrospheres (diameter >1 mm) with a more uniform size distribution (1.2–1.6 mm) were obtained using alginate and CH. On the other hand, macrospheres with larger size distribution were obtained using PLA (0.8–2 mm), which may affect their release rate of encapsulated factors and their injectability . Commercially, the size of BG in the form of grains varies with the function of the application.…”

Section: Resultsmentioning

confidence: 99%

“…BG grains in the 1–2 mm size range are indicated for orthopedic, trauma, and septic surgery. Microspheres (nHAp/PLGA) with approximately 250 μm exhibited bone-repair capacity . On the other hand, nanosized sphere sizes result in inadequate space for tissue and vascular ingrowth and for carrying bone forming cells. , In addition, typical45S5 bioactive glass products have irregular particles with a wide range of sizes.…”

Section: Resultsmentioning

confidence: 99%

“…The production of Alginate/BG macrospheres involved the extrusion method, which entailed combining the material to be encapsulated with a solution of an encapsulating material, such as sodium alginate. This mixture was thoroughly homogenized and subsequently extruded into a gelling solution, resulting in the formation of macrospheres. − Alginate can exchange ions with divalent ions to form a stable “egg-box” hydrogel owing to the unique ionotropic-gelation effect . The methodology used followed the series of steps reported elsewhere …”

Section: Methodsmentioning

confidence: 99%

“…BG were last encapsulated into the biodegradable polymer CH matrix producing polymer/ceramic composite macrospheres. This procedure is based on the ionotropic gelation technique. , CH is a nature-derived cationic polymer that can react with polyanions to prepare microspheres with a gel network …”

Section: Methodsmentioning

confidence: 99%

“…By means of microencapsulation techniques, substances in solid, liquid, or gas are surrounded with a coating material, typically a polymeric agent, allowing the controlled release of several bioactive compounds. , Spheres based on bioactive ceramics have been developed using polylactic acid (PLA), polyglycolic acid (PGA), gelatin, alginate, and chitosan (CH) due to their biocompatibility and complete bioresorbability. ,− Improvement in the properties of bioceramic microspheres as slow degradation rates and high brittleness is obtained for creating composite microspheres …”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Biocomposite Macrospheres Based on Strontium-Bioactive Glass for Application as Bone Fillers

de Oliveira,

dos Santos Gonçalves,

Wallace dos Santos

et al. 2023

ACS Mater. Au

View full text Add to dashboard Cite

Traditional bioactive glass powders are typically composed of irregular particles that can be packed into dense configurations presenting low interconnectivity, which can limit bone ingrowth. The use of novel biocomposite sphere formulations comprising bioactive factors as bone fillers are most advantageous, as it simultaneously allows for packing the particles in a 3-dimensional manner to achieve an adequate interconnected porosity, enhanced biological performance, and ultimately a superior new bone formation. In this work, we develop and characterize novel biocomposite macrospheres of Sr-bioactive glass using sodium alginate, polylactic acid (PLA), and chitosan (CH) as encapsulating materials for finding applications as bone fillers. The biocomposite macrospheres that were obtained using PLA have a larger size distribution and higher porosity and an interconnectivity of 99.7%. Loose apatite particles were observed on the surface of macrospheres prepared with alginate and CH by means of soaking into a simulated body fluid (SBF) for 7 days. A dense apatite layer was formed on the biocomposite macrospheres’ surface produced with PLA, which served to protect PLA from degradation. In vitro investigations demonstrated that biocomposite macrospheres had minimal cytotoxic effects on a human osteosarcoma cell line (SaOS-2 cells). However, the accelerated degradation of PLA due to the degradation of bioactive glass may account for the observed decrease in SaOS-2 cells viability. Among the biocomposite macrospheres, those composed of PLA exhibited the most promising characteristics for their potential use as fillers in bone tissue repair applications.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Biocomposite Macrospheres Based on Strontium-Bioactive Glass for Application as Bone Fillers

de Oliveira,

dos Santos Gonçalves,

Wallace dos Santos

et al. 2023

ACS Mater. Au

View full text Add to dashboard Cite

show abstract

In Situ Enzymatic Reaction Generates Magnesium‐Based Mineralized Microspheres with Superior Bioactivity for Enhanced Bone Regeneration

Cai¹,

Liu

Hu³

et al. 2023

Adv Healthcare Materials

Self Cite

View full text Add to dashboard Cite

Bone is a naturally mineralized tissue with a remarkable hierarchical structure, and the treatment of bone defects remains challenging. Microspheres with facile features of controllable size, diverse morphologies, and specific functions display amazing potentials for bone regeneration. Herein, inspired by natural biomineralization, a novel enzyme‐catalyzed reaction is reported to prepare magnesium‐based mineralized microspheres. First, silk fibroin methacryloyl (SilMA) microspheres are prepared using a combination of microfluidics and photo‐crosslinking. Then, the alkaline phosphatase (ALP)‐catalyzed hydrolysis of adenosine triphosphate (ATP) is successfully used to induce the formation of spherical magnesium phosphate (MgP) in the SilMA microspheres. These SilMA@MgP microspheres display uniform size, rough surface structure, good degradability, and sustained Mg2+ release properties. Moreover, the in vitro studies demonstrate the high bioactivities of SilMA@MgP microspehres in promoting the proliferation, migration, and osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs). Transcriptomic analysis shows that the osteoinductivity of SilMA@MgP microspheres may be related to the activation of the PI3K/Akt signaling pathway. Finally, the bone regeneration enhancement units (BREUs) are designed and constructed by inoculating BMSCs onto SilMA@MgP microspheres. In summary, this study demonstrates a new biomineralization strategy for designing biomimetic bone repair materials with defined structures and combination functions.

show abstract

Biomimetic Hydrogels as the Inductive Endochondral Ossification Template for Promoting Bone Regeneration

Zhao,

Qiu,

Liu

et al. 2024

Adv Healthcare Materials

View full text Add to dashboard Cite

Repairing critical size bone defects (CSBD) is a major clinical challenge and requires effective intervention by biomaterial scaffolds. Inspired by the fact that the cartilaginous template‐based endochondral ossification (ECO) process is crucial to bone healing and development, developing biomimetic biomaterials to promote ECO is recognized as a promising approach for repairing CSBD. With the unique highly hydrated 3D polymeric network, hydrogels can be designed to closely emulate the physiochemical properties of cartilage matrix to facilitate ECO. In this review, we first introduce the various preparation methods of hydrogels possessing the specific physiochemical properties required for promoting ECO. We further summarize the materiobiological impacts of the physicochemical properties of hydrogels, such as mechanical properties, topographical structures and chemical compositions on ECO and the associated molecular mechanisms related to the BMP, Wnt, TGF‐β, HIF‐1α, FGF and RhoA signaling pathways. This review provides a detailed coverage on the materiobiological insights required for the design and preparation of hydrogel‐based biomaterials to facilitate bone regeneration.This article is protected by copyright. All rights reserved

show abstract

Microspheres in bone regeneration: Fabrication, properties and applications

Cited by 18 publications

References 149 publications

Biocomposite Macrospheres Based on Strontium-Bioactive Glass for Application as Bone Fillers

Biocomposite Macrospheres Based on Strontium-Bioactive Glass for Application as Bone Fillers

In Situ Enzymatic Reaction Generates Magnesium‐Based Mineralized Microspheres with Superior Bioactivity for Enhanced Bone Regeneration

Biomimetic Hydrogels as the Inductive Endochondral Ossification Template for Promoting Bone Regeneration

Contact Info

Product

Resources

About