Microspore mutagenesis of <i>Brassica</i> species for fatty acid modifications: a preliminary evaluation

Ferrie, A. M. R.; Taylor, David C.; MacKenzie, S. L.; Rakow, G.; Raney, J. P.; Keller, W. A.

doi:10.1111/j.1439-0523.2008.01502.x

Cited by 42 publications

(29 citation statements)

References 29 publications

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“…Furthermore, bleomycin (0.1-0.2 μg/ml), a glycopeptide antibiotic, for 20-30 min pretreatment increased embryo yields in B. napus , (Zeng et al 2010 ;Ahmadi et al 2012 ). Some mutagens such as γ-rays irradiation, N-Nitroso-N-Methyl Urethane (NMU) and ethyl methane sulphonate (EMS) were reported to promote microspore embryogenesis in B. napus , B. oleracea and B. juncea (Jeung and Lee 1997 ;Pechan and Keller 1989 ;Ferrie et al 2008 ).…”

Section: Stress and Pretreatmentmentioning

confidence: 97%

Microspore Culture and Doubled Haploid Technology

Takahata

Takahashi

Tsuwamoto³

2013

Biotechnology of Crucifers

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Haploids and doubled haploids (DHs) produced by in vitro culture of gametophytic cells, especially male gametophyto, are of great importance to plant breeding and basic science. The routine protocol of isolated microspore culture of Brassica has been established, and used worldwide. However, recently, new protocols dealing with multiple samples have been developed. Although various factors infl uencing microspore embryogenesis, plant regeneration and diplodization have been examined, attempts to enhance these effi ciencies have continued. With the advancement of molecular genetics, many approaches from both forward-and reverse-genetics for understanding the mechanism of microspore embryogenesis have been attempted. This chapter reviews the recent progress of microspore embryogenesis of Brassica in relation to culture protocol and factors affecting microspore embryogenesis, and recent achievements in elucidating the mechanism of microspore embryogenesis.

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Section: Stress and Pretreatmentmentioning

confidence: 97%

Microspore Culture and Doubled Haploid Technology

Takahata

Takahashi

Tsuwamoto³

2013

Biotechnology of Crucifers

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“…Consequently, much of our knowledge of genetics of higher organisms is based upon works utilizing induced mutations for analyzing gene function (McCallum et al, 2000). To date, several welldocumented examples of successful applications of mutation breeding to oilseed crops have been reported in the literature Bacelis, 2001;Bhatia et al, 1999;Ferrie et al, 2008;Fowler and Stefansson, 1972;Kott et al, 1996;MacDonald et al, 1991;Newsholme et al, 1989;Osorio et al, 1995;Parry et al, 2009;Rowland, 1991;Sala et al, 2008;Schnurbush et al, 2000;Spasibionek, 2006;Swanson et al, 1989;Velasco et al, 2008). Induced mutations have been used mainly to generate variation that could rarely be found in germplasm collections.…”

Section: Introductionmentioning

confidence: 99%

“…Soybean with 155 mutant cultivars possesses the highest number of mutant cultivars, followed by sesame with 24 and canola with 15 cultivars. In canola, oil modification has been achieved by using seed and microspore mutagenesis (Ferrie et al, 2008;MacDonald et al, 1991;Velasco et al, 2008). In spring canola, radiation treatment has been applied to the seeds of "Regent" cultivar and M 5 lines selected with increased oleic acid contents varying from 63 to 79%.…”

Section: Introductionmentioning

confidence: 99%

Seed viability, germination and seedling growth of canola (Brassica napus L.) as influenced by chemical mutagens

Emrani¹,

Arzani²,

Saeidi³

2011

Afr. J. Biotechnol.

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Mutation induction is considered as an effective way to enrich plant genetic variation, particularly for traits with a very low level of genetic variation. The objectives of this study were to evaluate the effect of different dosages of chemical mutagens on seed germination, seed viability and seedling growth characteristics and to identify optimum treatment conditions for chemical mutagens based on the LD 50 criterion in canola (Brassica napus L.). Two pretreatment conditions of soaking in distilled water and non-soaking, different concentrations of chemical mutagens, and four treatment periods were investigated. The effect of mutagen dosage on seed viability was also assessed using the tetrazolium staining test. Results revealed the significant effects of mutagen dosages and treatment periods on seed viability and seed germination as well as on seedling characteristics for all the mutagens tested. Additionally, it was found that increased dosage and period in each treatment led to significant reductions in seed viability for the tested mutagens. Pretreatment did not significantly influence most of the studied characteristics. The 0.8% ethyl methanesulfonate (EMS) for 6 h, 12 mM N-nitroso-Nethylurea (ENU) and 6 mM sodium azide for 8 h and 9 mM N-nitroso-N-methylurea (NMU) for 4 h were considered as optimum treatment conditions.

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“…Traits are fixed and homozygous true breeding lines are produced within one generation, which can help reduce the time needed to develop new varieties (Thomas et al 2003;Ferrie and Caswell 2011;Ferrie and Möllers 2011). For the same reasons, in vitro microspore culture has become an important tool for mutagenesis (Barro et al 2001(Barro et al , 2002Sonntag and Rudloff 2004;Liu et al 2005;Ferrie et al 2008) and in vitro selection (Albrecht et al 1995;Möllers et al 2000;Liu et al 2005). In addition, microspores, MDEs and DH plants are being used for transformation studies.…”

Section: Introductionmentioning

confidence: 99%

Identification and evaluation of intervarietal substitution lines of rapeseed (Brassica napus L.) with donor segments affecting the diploidization rate of isolated microspores

et al. 2016

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In Brassica species microspore derived doubled haploid lines are an important tool in breeding and research. A limiting step in the production of doubled haploid lines is the diploidization of the microspores. Strong differences have been observed in diploidization rates between different genotypes but little is know about the genetic control of these differences. To identify genomic regions that carry genetic factors controlling the diploidization rate of isolated microspores of rapeseed, marker segregations were compared between segregating populations of diploid and haploid microspore derived embryos and a BC 1 from a cross between 'Express 617' and 'RS239'. After map construction ten intervarietal substitution lines from the same cross were selected with donor segments covering five genomic regions that showed a pattern of skewed marker segregations across the three populations indicative of the segregation of genes controlling the diploidization rates. The diploidization rates of microspores of the ten lines ranged from 23.9 to 58.7 % while the recurrent parent 'Express 617' showed a rate of 52.5 %. For three lines the diploidization rates were significantly lower (P = 0.05) than the rate of 'Express 617'. By comparing donor segments between the significant and the non-significant lines, seven genomic regions that cover just between 4.17 and 6.16 % of the rapeseed genome were identified that may contain genetic factors controlling diploidization rates in rapeseed. In addition, one marker was found that has a high probability to be linked to such a factor. The significant lines represent an ideal material for further in depth studies of this trait.

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Microspore mutagenesis of Brassica species for fatty acid modifications: a preliminary evaluation

Cited by 42 publications

References 29 publications

Microspore Culture and Doubled Haploid Technology

Microspore Culture and Doubled Haploid Technology

Seed viability, germination and seedling growth of canola (Brassica napus L.) as influenced by chemical mutagens

Identification and evaluation of intervarietal substitution lines of rapeseed (Brassica napus L.) with donor segments affecting the diploidization rate of isolated microspores

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