“…One of the methods to change the structure and composition of plant tissues is vacuum impregnation (VI) (Derossi & Severini, 2012;Fito, Chiralt, Barat, Spiess, & Behsnilian, 2001), which has been shown to improve the quality of frozen-thawed fruits and vegetables by incorporating stabilizers such as pectin (Reno, Prado, & Resende, 2011;Xie & Zhao, 2004) or zinc and calcium salts (Xie & Zhao, 2004) into the structure of the commodity prior to freezing. Impregnation of cryoprotectants, such as antifreeze proteins (Velickova et al, 2013), and sugars, such as sucrose (Ursachia, Segalb, & Dicu, 2009) and trehalose (Velickova et al, 2013), have been reported to have a positive effect on the quality of the food products after freezing and thawing.…”