Scuffing is a catastrophic failure that causes significant surface damages such as plastic flow and welding with a marked increase in friction, wear, temperature and noise. In this study, variations in the crystal grain structure of a steel surface was analysed in situ during the scuffing process using a synchrotron X-ray diffraction system, combined with a visible camera and a near-infrared thermometer. The in situ observation system was synchronously operated to capture a contact area between a rotating sapphire ring and a stationary bearing steel pin. The Debye-Scherrer ring diffracted from the contact area was captured by a two-dimensional detector. The scuffing behaviour could be classified as either micro scuffing or macro scuffing. During the micro scuffing period, plastic flow occurred intermittently with a significant temperature rise of approximately 1000 °C. During the macro scuffing period, heat was continuously generated over the contact area. When plastic flow occurred, the captured Debye-Scherrer ring indicated the orientation of crystal grains as well as a phase transformation from martensite to austenite. This study constitutes the first in-situ observation of the behaviour of crystal grains in the dynamic recrystallisation process occurring during the scuffing process.