Microstructural interactions contribute to the hotspot in the living cochlea

Liu, Junpei; Bai, Yanru; Cheng, Qianli; Zheng, Shu Xian; Elliott, Stephen J.; Ni, Guangjian

doi:10.1016/j.crneur.2022.100045

Cited by 3 publications

(1 citation statement)

References 36 publications

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“…It also remains to be determined if cochlea amplification of 44 kHz voltage responses measured at the 53 kHz location that are suppressed by HOP light-activated hyperpolarization of DCs and OPCs is due all or in part to block of apical transfer of forces from the 44 kHz region (Figure 4). Movement from remote sources could reach the measurement site on the BM through longitudinal transmission of forces along the cochlear partition, which might implicate a role for supporting cells, including OPCs and DCs as proposed in "feedforward" models (Geisler and Sang,1995;Russell and Nilson, 1997;Steele et al, 1999;Liu et al, 2022). These forces do not excite OHCs, which respond to radial shear between the TM and RL and have tuning curves resembling those of the BM (Russell and Kössl, 1992;Russell et al, 1995) and without the broad frequency characteristics reported for the frequency tuning of the RL (He et al, 2018;Ren et al, 2016) the "hotspot" within the OoC (Cooper et al, 2018) which may be generated by flow of forces along the OoC length.…”

Section: Hop Light-activated Hyperpolarization Of Dcs and Opcs Impair...mentioning

confidence: 99%

Optogenetics Reveals Roles for Supporting Cells in Force Transmission to and From Outer Hair Cells in the Mouse Cochlea

Lukashkina,

Levic,

Simões

et al. 2023

J. Neurosci.

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Outer hair cells (OHCs) of the organ of Corti (OoC), acting as bidirectional cellular mechanoelectrical- transducers, generate, receive, and exchange forces with other major elements of the cochlear partition, including the sensory inner hair cells (IHCs). Force exchange is mediated via a supporting cell scaffold, including Deiters’ (DC) and outer pillar cells (OPC), to enable the sensitivity and exquisite frequency selectivity of the mammalian cochlea and to transmit its responses to the auditory nerve. To selectively activate DCs and OPCs in male and female mice, we conditionally expressed in them a hyperpolarizing halorhodopsin (HOP), a light-gated inward chloride ion pump and measured extracellular receptor potentials (ERPs) and their DC component (ERPDCs) from the Cortilymph, which fills the OoC fluid spaces, and compared the responses with similar potentials from HOP-/-littermates. The compound action potentials (CAP) of the auditory nerve were measured as an indication of IHC activity and transmission of cochlear responses to the CNS. HOP light-activated hyperpolarization of DCs and OPCs suppressed cochlear amplification through changing timing of its feedback, altered basilar membrane (BM) responses to tones at all measured levels and frequencies, and reduced IHC excitation. HOP-activation findings reported here complement recent studies that revealed channelrhodopsin activation depolarized DCs and OPCs and effectively bypassed, rather than blocked, the control of OHC mechanical and electrical responses to sound and their contribution to timed and directed electromechanical feedback to the mammalian cochlea. Moreover, our findings identify DCs and OPCs as potential targets for the treatment of noise-induced hearing loss.Significance StatementOuter hair cells provide electromechanical feedback to the organ of Corti, mediated via a cellular scaffold of Deiters’ and outer pillar cells, that enables the sensitivity and fine frequency tuning of the cochlea. The role of this scaffold was explored by expressing the halorhodopsin HOP in Deiters’ and pillar cells of male and female mice which became hyperpolarized when illuminated. HOP light-activated hyperpolarization suppressed cochlear amplification, altered basilar membrane responses to tones, including those at levels and frequencies not subject to amplification, and attenuated neural excitation. The findings indicate supporting cells in mediating force transmission between outer hair cells and the organ of Corti and as targets for hearing loss treatments.

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Section: Hop Light-activated Hyperpolarization Of Dcs and Opcs Impair...mentioning

confidence: 99%

Optogenetics Reveals Roles for Supporting Cells in Force Transmission to and From Outer Hair Cells in the Mouse Cochlea

Lukashkina,

Levic,

Simões

et al. 2023

J. Neurosci.

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Towards simulating micromechanics of the organ of Corti induced by the traveling wave using a slice finite-element model of the mouse cochlea

Wang,

Puria

2024

NONLINEARITY AND HEARING: ADVANCES IN THEORY AND EXPERIMENT: Proceedings of the 14th International Mechanics of Hearing Worksho

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Optogenetics reveals roles for supporting cells in force transmission to and from outer hair cells in the mouse cochlea

Lukashkina

Levic

Simões

et al. 2023

Preprint

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Cochlear outer hair cells (OHCs), acting as bidirectional cellular mechanoelectrical transducers, generate, receive, and exchange forces with other major elements of the cochlear partition, including inner hair cells (IHCs). Force exchange is mediated via a supporting cell scaffold, including Deiters (DC) and outer pillar cells (OPC), to enable the sensitivity and exquisite frequency selectivity of the mammalian cochlea. We conditionally expressed a hyperpolarizing halorhodopsin (HOP), a light-gated inward chloride ion pump in DCs and OPCs. We measured extracellular receptor potentials (ERPs) and their DC component (ERPDC) from the Cortilymph (CL) of HOP expressing mice and compared the responses with similar potentials from littermates without HOP expression. Compound action potentials (CAP) were measured as an indication of IHC activity. HOP laser activation suppressed cochlear amplification through changing timing of its feedback, altered basilar membrane (BM) responses to tones at all measured levels and frequencies, and reduced IHC excitation. Our HOP activation results here complement previous channelrhodopsin activation studies in exploiting optogenetics to measure and understand the roles of DCs and OPCs in vivo in controlling the mechanical and electrical responses of OHCs to sound and their contribution to timed and directed electromechanical feedback to the mammalian cochlea.

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Microstructural interactions contribute to the hotspot in the living cochlea

Cited by 3 publications

References 36 publications

Optogenetics Reveals Roles for Supporting Cells in Force Transmission to and From Outer Hair Cells in the Mouse Cochlea

Optogenetics Reveals Roles for Supporting Cells in Force Transmission to and From Outer Hair Cells in the Mouse Cochlea

Towards simulating micromechanics of the organ of Corti induced by the traveling wave using a slice finite-element model of the mouse cochlea

Optogenetics reveals roles for supporting cells in force transmission to and from outer hair cells in the mouse cochlea

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