Abstract. Crystallographic preferred orientations (CPOs) are particularly important in controlling the mechanical properties of glacial shear margins. Logistical and safety considerations often make direct sampling of shear margins difficult and geophysical measurements are commonly used to constrain the CPOs. We present here the first direct comparison of seismic and ultrasonic data with measured CPOs in a polar shear margin. The measured CPO from ice samples from a 58 m deep borehole in the left lateral shear margin of the Priestley Glacier, Antarctica, is dominated by horizontal c-axes aligned sub-perpendicular to flow. A vertical seismic profile experiment with hammer shots up to 50 m away from the borehole, in four different azimuthal directions, shows velocity anisotropy of both P-waves and S-waves. Matching P-wave data to the anisotropy corresponding to CPO models defined by horizontally aligned c-axes gives two possible solutions for c-axis azimuth, one of which matches the c-axis measurements. If both P-wave and S-wave data are used, there is one best fit for azimuth and intensity of c-axis alignment that matches well the measurements. Azimuthal P-wave and S-wave ultrasonic data recorded in the laboratory on the ice core show clear anisotropy that matches that predicted from the CPO of the samples. With good quality data, azimuthal increments of 30° or less will constrain well the orientation and intensity of c-axis alignment. Our experiments provide a good framework for planning seismic surveys aimed at constraining the anisotropy of shear margins.