Effect of ultrasonic vibration (USV) treatment on microstructure and mechanical properties of brass alloy has been investigated. Microstructure of the investigated brass alloy without USV treatment exhibited stretched and dendritic α-phase within an average length of 165 µm and also interdendritic coarse β-phase. By applying USV treatment, the α-phase is significantly refined that obtained an average length of 21 µm. In addition, USV treatment achieved more homogenized structure of α and β-phases. The refinement of microstructure enhanced of course the mechanical properties of the brass alloy including yield strength, ultimate tensile strength, elongation, hardness and wear resistance. Furthermore, fracture surfaces and worn surfaces mechanisms were also discussed.