Due to their good oxidation resistance, high heatresistant capability and good mechanical properties, Ni-based superalloys have been widely used for preparing the parts of aero-engine, such as turbine guide-vane, turbine disks and other bearing parts [1][2][3] . The microstructure of the Ni-based superalloy mainly consists of γ matrix, γ' phase and carbides [4][5][6] . To increase the solid solution strengthening level of the alloy, alloying elements with high melting points, such as W, Re, Ru, and so on, are commonly added during composition designs [7] . However, the application of the nickel-based superalloys containing Re and Ru is limited, mainly due to the prices of these two elements being much more expensive than the others. The element W, by contrast, has a lower price and could well improve the high temperature properties of the alloy [8,9] , which is thought to be an ideal alloying element to some extent to replace Re and Ru [10] . W mainly distributes in γ matrix Abstract: K416B Ni-based superalloy with high W content has good high temperature properties and low cost, which has a great development potential. To investigate the room temperature tensile property and the deformation feature of K416B superalloy, tensile testing at room temperature was carried out, and optical microscopy (OM), scanning electron microscopy (SEM) and transmission electron microscopy (TEM) were used to analyze the deformation and damage mechanisms. Results show that the main room temperature tensile deformation features of the K416B nickel-based superalloy are dislocations slipping in the matrix and shearing into γ' phase. The <110> super-dislocations shearing into γ' phase can form the anti-phase boundary two coupled (a/2)<110> partial-dislocations or decompose into the configuration of two (a/3)<112> partial dislocations plus stacking fault. In the later stage of tensile testing, the slip-lines with different orientations are activated in the grain, causing the stress concentration in the regions of block carbide or the porosity, and cracks initiate and propagate along these regions.