Microstructure of early embryonic aortic arch and its reversibility following mechanically altered hemodynamic load release

Celik, Merve; Goktas, Selda; Karakaya, Cansu; Çakıroğlu, Ayşe İdil; Karahüseyinoğlu, Serçin; Lashkarinia, S Samaneh; Ermek, Erhan; Pekkan, Kerem

doi:10.1152/ajpheart.00495.2019

Cited by 14 publications

(9 citation statements)

References 40 publications

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“…Numerous research teams have quantified intracardiac biomechanical loading conditions (blood flow, 3D and 4D shear stresses, strains) during normal AV valve ( Figure 10 ) [ 136 , 145 , 146 , 147 , 148 , 149 , 150 , 151 , 152 , 153 , 154 , 155 ], outflow tract [ 156 , 157 , 158 , 159 , 160 ], and aortic arch morphogenesis ( Figure 11 ) [ 34 , 161 , 162 , 163 , 164 , 165 , 166 , 167 ]. The impact of altered biomechanical loading conditions on cardiac and vascular morphogenesis ( Figure 12 ) has confirmed altered intracardiac blood flow as one etiology for CHD [ 135 , 165 , 168 , 169 , 170 , 171 , 172 , 173 , 174 , 175 ]. Increased ventricular loading associated with CT banding alters ventricular gene expression and mitral valve morphogenesis [ 176 ].…”

Section: Embryonic Ventricular and Vascular Biomechanicsmentioning

confidence: 97%

Validating the Paradigm That Biomechanical Forces Regulate Embryonic Cardiovascular Morphogenesis and Are Fundamental in the Etiology of Congenital Heart Disease

Keller

Kowalski

Tinney

et al. 2020

JCDD

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The goal of this review is to provide a broad overview of the biomechanical maturation and regulation of vertebrate cardiovascular (CV) morphogenesis and the evidence for mechanistic relationships between function and form relevant to the origins of congenital heart disease (CHD). The embryonic heart has been investigated for over a century, initially focusing on the chick embryo due to the opportunity to isolate and investigate myocardial electromechanical maturation, the ability to directly instrument and measure normal cardiac function, intervene to alter ventricular loading conditions, and then investigate changes in functional and structural maturation to deduce mechanism. The paradigm of “Develop and validate quantitative techniques, describe normal, perturb the system, describe abnormal, then deduce mechanisms” was taught to many young investigators by Dr. Edward B. Clark and then validated by a rapidly expanding number of teams dedicated to investigate CV morphogenesis, structure–function relationships, and pathogenic mechanisms of CHD. Pioneering studies using the chick embryo model rapidly expanded into a broad range of model systems, particularly the mouse and zebrafish, to investigate the interdependent genetic and biomechanical regulation of CV morphogenesis. Several central morphogenic themes have emerged. First, CV morphogenesis is inherently dependent upon the biomechanical forces that influence cell and tissue growth and remodeling. Second, embryonic CV systems dynamically adapt to changes in biomechanical loading conditions similar to mature systems. Third, biomechanical loading conditions dynamically impact and are regulated by genetic morphogenic systems. Fourth, advanced imaging techniques coupled with computational modeling provide novel insights to validate regulatory mechanisms. Finally, insights regarding the genetic and biomechanical regulation of CV morphogenesis and adaptation are relevant to current regenerative strategies for patients with CHD.

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Section: Embryonic Ventricular and Vascular Biomechanicsmentioning

confidence: 97%

Validating the Paradigm That Biomechanical Forces Regulate Embryonic Cardiovascular Morphogenesis and Are Fundamental in the Etiology of Congenital Heart Disease

Keller

Kowalski

Tinney

et al. 2020

JCDD

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“…They observed that the midpoint diameters of the right arches are greater than the left and are in the range of 0.101 to 0.129 mm for HH18 and 0.113 to 0.138 mm for HH24, respectively [73]. Another study by Celik et al evaluated the aortic arch diameters; at HH24, the maximum diameter was ~0.3 mm [74]. Lindsey et al studied the diameter upon occlusion at HH18 and HH24 and found it to be ~0.2 mm [75].…”

Section: Aortic Arch and Vitelline Artery Properties Geometrymentioning

confidence: 99%

Soft-Tissue Material Properties and Mechanogenetics during Cardiovascular Development

Siddiqui

Dogru

Lashkarinia

et al. 2022

JCDD

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During embryonic development, changes in the cardiovascular microstructure and material properties are essential for an integrated biomechanical understanding. This knowledge also enables realistic predictive computational tools, specifically targeting the formation of congenital heart defects. Material characterization of cardiovascular embryonic tissue at consequent embryonic stages is critical to understand growth, remodeling, and hemodynamic functions. Two biomechanical loading modes, which are wall shear stress and blood pressure, are associated with distinct molecular pathways and govern vascular morphology through microstructural remodeling. Dynamic embryonic tissues have complex signaling networks integrated with mechanical factors such as stress, strain, and stiffness. While the multiscale interplay between the mechanical loading modes and microstructural changes has been studied in animal models, mechanical characterization of early embryonic cardiovascular tissue is challenging due to the miniature sample sizes and active/passive vascular components. Accordingly, this comparative review focuses on the embryonic material characterization of developing cardiovascular systems and attempts to classify it for different species and embryonic timepoints. Key cardiovascular components including the great vessels, ventricles, heart valves, and the umbilical cord arteries are covered. A state-of-the-art review of experimental techniques for embryonic material characterization is provided along with the two novel methods developed to measure the residual and von Mises stress distributions in avian embryonic vessels noninvasively, for the first time in the literature. As attempted in this review, the compilation of embryonic mechanical properties will also contribute to our understanding of the mature cardiovascular system and possibly lead to new microstructural and genetic interventions to correct abnormal development.

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“…The chicken embryo models have been used in the study of teratogenicity ( 79 ), disease development ( 39 , 80 ), cell migration and histogenesis, and causal mechanisms of neurocrystopathies (abnormal specification, migration, differentiation, or death of neural crest cells during embryonic development) ( 81 ). In ovo transplantation of neural crest cells (NC) derived from keratinocyte cells (KC) showed that these NC-KC cells could migrate to the neural crest region, grow within the egg, and produce different NC derivates, which is the indication of maintaining their pluripotent state ( 82 ).…”

Section: Applications Of In Ovo Technologymentioning

confidence: 99%

In ovo Feeding as a Tool for Improving Performance and Gut Health of Poultry: A Review

2021

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Early growth and development of the gastrointestinal tract are of critical importance to enhance nutrients' utilization and optimize the growth of poultry. In the current production system, chicks do not have access to feed for about 48–72 h during transportation between hatchery and production farms. This lag time affects early nutrient intake, natural exposure to the microbiome, and the initiation of beneficial stimulation of the immune system of chicks. In ovo feeding can provide early nutrients and additives to embryos, stimulate gut microflora, and mitigate the adverse effects of starvation during pre-and post-hatch periods. Depending on the interests, the compounds are delivered to the embryo either around day 12 or 17 to 18 of incubation and via air sac or amnion. In ovo applications of bioactive compounds like vaccines, nutrients, antibiotics, prebiotics, probiotics, synbiotics, creatine, follistatin, L-carnitine, CpG oligodeoxynucleotide, growth hormone, polyclonal antimyostatin antibody, peptide YY, and insulin-like growth factor-1 have been studied. These compounds affect hatchability, body weight at hatch, physiological functions, immune responses, gut morphology, gut microbiome, production performance, and overall health of birds. However, the route, dose, method, and time of in ovo injection and host factors can cause variation, and thereby inconsistencies in results. Studies using this method have manifested the benefits of injection of different single bioactive compounds. But for excelling in poultry production, researchers should precisely know the proper route and time of injection, optimum dose, and effective combination of different compounds. This review paper will provide an insight into current practices and available findings related to in ovo feeding on performance and health parameters of poultry, along with challenges and future perspectives of this technique.

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Microstructure of early embryonic aortic arch and its reversibility following mechanically altered hemodynamic load release

Cited by 14 publications

References 40 publications

Validating the Paradigm That Biomechanical Forces Regulate Embryonic Cardiovascular Morphogenesis and Are Fundamental in the Etiology of Congenital Heart Disease

Validating the Paradigm That Biomechanical Forces Regulate Embryonic Cardiovascular Morphogenesis and Are Fundamental in the Etiology of Congenital Heart Disease

Soft-Tissue Material Properties and Mechanogenetics during Cardiovascular Development

In ovo Feeding as a Tool for Improving Performance and Gut Health of Poultry: A Review

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