Microstructured Tungsten Oxide: A Generic Desorption/Ionization Substrate for Mass Spectrometry

Yang, Xiujuan; Hu, X. K.; Лобода, А. В.; Lipson, R. H.

doi:10.1002/adma.201001627

Cited by 18 publications

(18 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…4 Since then a large number of nanostructured materials have been explored for surface and particle based LDI-MS including gold nanoparticles, 5-10 nanostructured gold 11 or platinum 12 surfaces and nanoparticles, 13 germanium oxide surfaces, 14 etched porous silicon, 2,4,15,16 silicon nanowires 17 and nanoposts, 18 the carbon allotropes 19 fullerene, 20 graphite, 21 graphene, 22 or semiconductor metal and metal oxides 23 among many others. 4 Since then a large number of nanostructured materials have been explored for surface and particle based LDI-MS including gold nanoparticles, 5-10 nanostructured gold 11 or platinum 12 surfaces and nanoparticles, 13 germanium oxide surfaces, 14 etched porous silicon, 2,4,15,16 silicon nanowires 17 and nanoposts, 18 the carbon allotropes 19 fullerene, 20 graphite, 21 graphene, 22 or semiconductor metal and metal oxides 23 among many others.…”

Section: Introductionmentioning

confidence: 99%

Nanostructured weathering steel for matrix-free laser desorption ionisation mass spectrometry and imaging of metabolites, drugs and complex glycans

Etxebarría

Calvo

Reichardt

2014

Analyst

View full text Add to dashboard Cite

Weathering steel has been employed for the first time to prepare sample plates for matrix-free laser desorption ionisation mass spectrometry (LDI-MS) of small molecules up to a mass range of around 1500 Da. The effective UV absorption, heat conductivity and porosity of the nanostructured inner rust layer formed during passivation determine the excellent performance in LDI-MS for a broad range of different analyte classes. The inexpensive material was evaluated in a series of relevant analytical applications ranging from the matrix-free detection of serum metabolites, lactose quantification, lipid analysis in milk to the glycoprofiling of antibodies and imaging mass spectrometry of brain tissue samples.

show abstract

Section: Introductionmentioning

confidence: 99%

Nanostructured weathering steel for matrix-free laser desorption ionisation mass spectrometry and imaging of metabolites, drugs and complex glycans

Etxebarría

Calvo

Reichardt

2014

Analyst

View full text Add to dashboard Cite

show abstract

“…These included microparticle species of WO 3 , WO 2 , and ReO 3 , in addition to nanoparticle size WO 3 . In previous studies, the work functions of rhenium and tungsten metals in oxygen‐rich environments were found to be very high, in the range of 6.4 and 6.0 eV, respectively, and surfaces of those metals provided promising platforms for ionization due to these work functions . These higher work functions are also conducive to photodetachment.…”

Section: Introductionmentioning

confidence: 97%

Laser desorption ionization of small molecules assisted by tungsten oxide and rhenium oxide particles

2015

View full text Add to dashboard Cite

Inorganic metal oxides have shown potential as matrices for assisting in laser desorption ionization (LDI) with advantages over the aromatic acids typically used. Rhenium and tungsten oxides are an attractive option due to their high work functions and relative chemical inertness. In this work, it is shown that ReO3 and WO3, in microparticle (μP) powder forms, can efficiently ionize various types of small molecules and provide minimized background contamination at analyte concentrations below 1 ng/μL. This study shows that untreated inorganic WO3 and ReO3 particles are valid matrix options for detection of protonatable, radical, and precharged species under LDI. Qualitatively, the WO3 μP showed an improved detection of apigenin, sodiated glucose, and the precharged analyte choline, while the ReO3 μP allowed detection of protonated cocaine, quinuclidine, ametryn, and radical ions of polyaromatic hydrocarbons at detection levels as low as 50 pg/μL. For thermometer ion survival yield experiments, it was also shown that the ReO3 powder was significantly softer than CCA. Furthermore, it provided higher intensities of cocaine and polyaromatic hydrocarbons, at laser flux values equal to that used with CCA.

show abstract

“…

Since the discovery of desorption ionization on porous silicon (DIOS), intact laser desorption ionization (LDI) of thermally labile molecules from nanostructured surfaces has been the subject of extensive investigations. 8 For this study, length-controlled WO 3 surface nanowires with a 50 nm diameter were prepared by utilizing anodic Al 2 O 3 templates. However, with recent advances in nanostructure fabrication, a variety of surface nanostructures and materials have been examined and some of them exhibited their LDI capability extending beyond the smallmolecule regime.

…”

mentioning

confidence: 99%

“…The high melting points of metal oxides may offer robust structures against laser ablation for this application. 8 For this study, length-controlled WO 3 surface nanowires with a 50 nm diameter were prepared by utilizing anodic Al 2 O 3 templates. However, investigations of LDI from metal oxides are rare.…”

mentioning

confidence: 99%

Observation of Intact Desorption Ionization of Peptide Molecules from Arrays of Tungsten Oxide Nanowires by Laser Irradiation

Han

2015

Bulletin Korean Chem Soc

View full text Add to dashboard Cite

Since the discovery of desorption ionization on porous silicon (DIOS), intact laser desorption ionization (LDI) of thermally labile molecules from nanostructured surfaces has been the subject of extensive investigations. 1,2 In fact, early studies focused on the ionization of small molecules because the use of excess matrices in MALDI (matrix-assisted LDI) has certain limitations in the low-mass region (m/z < 400), including matrix peak interference. However, with recent advances in nanostructure fabrication, a variety of surface nanostructures and materials have been examined and some of them exhibited their LDI capability extending beyond the smallmolecule regime. Now a possible realization of matrix-free LDI as a true alternative to conventional MALDI for protein mass spectrometry can be anticipated.Although the mechanism of LDI on the surface of nanostructures is too complex to understand fully, some previous studies showed that intact desorption might be promoted by rapid surface heating caused by momentary trapping of absorbed laser energy within the nanostructures before it dissipates into the bulk substrates. 3-5 For example, LDI can cause a rapid increase in surface temperature to 1000 K or more, in a 10-ns time scale for Si surface nanostructures.Among the many surface materials that might enhance LDI, we chose metal oxide nanowires in this study. The high melting points of metal oxides may offer robust structures against laser ablation for this application. In addition, reproducible fabrication of nanostructures by using metal oxides is well known. However, investigations of LDI from metal oxides are rare. A few studies on zinc oxide (ZnO) nanowires revealed limited LDI performance for small drug molecules. But LDI of large molecules, such as peptides, were not successful at all from the ZnO nanostructured surfaces. 4,6 Herein, we report the observation of pronounced LDI of peptide molecules from the arrays of tungsten oxide (WO 3 ) nanowires, which possess promising material properties for the LDI mechanism based on rapid surface heating. WO 3 is a semiconductor (bandgap~2.73 eV) with a high melting point (1473 C), which is suitable for high-power UV laser irradiation. Its very low thermal conductivity of 1.63 W m −1 K −1 may promote the rapid surface heating required for intact desorption of thermally labile molecules, which is much lower than for Si (150 W m −1 K −1 ) and smaller still than for amorphous Si (5.5 W m −1 K −1 ). 5 Although ZnO nanowires were not successful for LDI of peptides, 4,6 a few previous studies including LDI using WO 3 nanoparticles as a matrix alternative 7 and LDI from rough WO 3 surfaces with micrometer dimensions suggest that WO 3 is a promising material for LDI surfaces. 8 For this study, length-controlled WO 3 surface nanowires with a 50 nm diameter were prepared by utilizing anodic Al 2 O 3 templates. Careful control of the fabrication process yielded a set of length-controlled nanowire arrays (Figure 1). The lengths of the nanowires covered a range of 60-250 nm. Typic...

show abstract

Microstructured Tungsten Oxide: A Generic Desorption/Ionization Substrate for Mass Spectrometry

Cited by 18 publications

References 28 publications

Nanostructured weathering steel for matrix-free laser desorption ionisation mass spectrometry and imaging of metabolites, drugs and complex glycans

Nanostructured weathering steel for matrix-free laser desorption ionisation mass spectrometry and imaging of metabolites, drugs and complex glycans

Laser desorption ionization of small molecules assisted by tungsten oxide and rhenium oxide particles

Observation of Intact Desorption Ionization of Peptide Molecules from Arrays of Tungsten Oxide Nanowires by Laser Irradiation

Contact Info

Product

Resources

About