A comparative study was undertaken to investigate the Nd:YAG and CO2 laser systems for laser-assisted vasovasostomies (LAVs). In 32 rats 64 vasovasostomies were performed, either conventionally sutured (CSV) or laser-welded (LAV-CO2 and LAV-Nd:YAG). Postoperative investigations included patency tests, gross examination, light and electron microscopy. The highest rate of sperm granulomas (50%) and the lowest patency rate (50%) was seen in the LAV-Nd:YAG group. LAV-CO2 showed the lowest rate of sperm granulomas (12.5%) and had a patency rate (82%) which was comparable to that of CSV (88%). Laser-assisted vas anastomosis is time saving and technically easy to perform. Contrary to microvascular anastomoses, where both laser types offer the same results, vas anastomosis is better done using a CO2 laser system.