Microsystems Technology and Biosensing

Sathuluri, Ramachandra Rao; Yamamura, Shohei; Tamiya, Eiichi

doi:10.1007/10_2007_078

Cited by 10 publications

(11 citation statements)

References 189 publications

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“…Various planar microreactor array type platforms have also been developed for PCR323334353637383940414243 and other life science applications444546474849505152 but none use the unique microfluidic configuration presented here. Cell-free in vitro protein expression has been done in various microreactor formats53545556575859 but never using the present configuration for filling and sealing the microreactors.…”

Section: Discussionmentioning

confidence: 99%

Microreactor Array Device

Wiktor

Brunner

Kahn

et al. 2015

Sci Rep

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We report a device to fill an array of small chemical reaction chambers (microreactors) with reagent and then seal them using pressurized viscous liquid acting through a flexible membrane. The device enables multiple, independent chemical reactions involving free floating intermediate molecules without interference from neighboring reactions or external environments. The device is validated by protein expressed in situ directly from DNA in a microarray of ~10,000 spots with no diffusion during three hours incubation. Using the device to probe for an autoantibody cancer biomarker in blood serum sample gave five times higher signal to background ratio compared to standard protein microarray expressed on a flat microscope slide. Physical design principles to effectively fill the array of microreactors with reagent and experimental results of alternate methods for sealing the microreactors are presented.

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Section: Discussionmentioning

confidence: 99%

Microreactor Array Device

Wiktor

Brunner

Kahn

et al. 2015

Sci Rep

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“…In recent years, miniaturized lab-on-chip and micro total analysis systems have been introduced for biochemical analyses in drug screening and the development of novel therapies using a variety of micro-and nano-technologies [22], [23]. The fluid handling capabilities of these miniaturized biosensor arrays provide significant advantages in terms of cost and throughput.…”

Section: System Architecturementioning

confidence: 99%

“…To ensure most of the voltage drops across , SW has to be large to minimize resistance [23]. To achieve this goal, m was selected for SW.…”

Section: A Thermal Controllermentioning

confidence: 99%

Thermally Controlled Electrochemical CMOS Microsystem for Protein Array Biosensors

Liu

Mason

2014

IEEE Trans. Biomed. Circuits Syst.

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Because many proteins useful in biosensors exhibit temperature dependent activity, this paper explores the opportunity to integrate thermal control within a protein array biosensor microsystem. A CMOS microhotplate array tailored to protein interfaces was developed for thermoregulation in a liquid sample environment. The microhotplates were shown to provide suitable thermal control for biosensor temperature ranges without the process complexity of most previously reported microhotplates. When combined with a CMOS analog thermal controller, the on-chip array was shown to set and hold temperatures for each protein site within ±1(°) C, and array elements were found to be almost completely thermally isolated from each other at distances beyond 0.4 mm. The compact size and low power of this controller enable it to be combined with the thermal control structures and instantiated for every element in a sensor array to increase biosensor interrogation throughput.

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“…The biological components of these systems permit operation at nearly physiological conditions, and the specificity and activity of the enzymes eliminates the need for electrode compartmentalization. The development of these systems is still maturing, but it is clear that these devices have a bright future as micro and nanoscale applications advance (Newman and Setford, 2006;Sathuluri et al, 2008;Willner and Willner, 2001).…”

Section: Introductionmentioning

confidence: 99%

Metabolic control analysis of an enzymatic biofuel cell

Glykys

Banta

2008

Biotech & Bioengineering

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Metabolic control analysis (MCA) is an analytical technique that aims to quantify the distribution of control that enzymes exhibit over the steady-state fluxes through a metabolic network. In an enzymatic biofuel cell, the flux of interest is the electrical current generated by the system. Regardless of transport limitations and other constraints, kinetic limitations can become potential bottlenecks in the operation of a biofuel cell. We have used an indirect approach to MCA to investigate a common osmium-mediated glucose oxidase/laccase enzymatic biofuel cell. The results of the analysis show that the control of the electron flux strongly depends on the total mediator concentrations and the extent of polarization of the individual electrodes. The effect of varying oxygen concentrations is also examined, as oxygen is required for the cathode, but it participates in a non-productive reaction at the anode. Under normal operating conditions the electrodes will be highly polarized and will both contain high mediator concentrations. This configuration will result in a dominant FCC at the anode, and the conditions that are needed for balanced flux control between the anode and cathode are explored. As increasingly complex bioelectrocatalytic systems and architectures are envisioned, MCA will be a valuable framework to facilitate their design and subsequent operation.

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Microsystems Technology and Biosensing

Cited by 10 publications

References 189 publications

Microreactor Array Device

Microreactor Array Device

Thermally Controlled Electrochemical CMOS Microsystem for Protein Array Biosensors

Metabolic control analysis of an enzymatic biofuel cell

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