Microtechnology for Mimicking In Vivo Tissue Environment

Sung, Jong Hwan; Shuler, Michael L.

doi:10.1007/s10439-011-0491-2

Cited by 52 publications

(46 citation statements)

References 88 publications

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“…[1][2][3][4] The class of materials known as hydrogels, which range from synthetic molecules such as poly(ethylene glycol) to native proteins, such as collagen and fibrin, has been demonstrated to be well-suited for use as 3D scaffolds. [5][6][7][8][9] Collagen-based hydrogels are gaining widespread popularity as scaffolds for tissue engineering due to the abundance of collagen in natural extracellular matrix (ECM).…”

Section: Introductionmentioning

confidence: 99%

Review of Collagen I Hydrogels for Bioengineered Tissue Microenvironments: Characterization of Mechanics, Structure, and Transport

Antoine

Vlachos

Rylander³

2014

Tissue Engineering Part B: Reviews

477

353

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Type I collagen hydrogels have been used successfully as three-dimensional substrates for cell culture and have shown promise as scaffolds for engineered tissues and tumors. A critical step in the development of collagen hydrogels as viable tissue mimics is quantitative characterization of hydrogel properties and their correlation with fabrication parameters, which enables hydrogels to be tuned to match specific tissues or fulfill engineering requirements. A significant body of work has been devoted to characterization of collagen I hydrogels; however, due to the breadth of materials and techniques used for characterization, published data are often disjoint and hence their utility to the community is reduced. This review aims to determine the parameter space covered by existing data and identify key gaps in the literature so that future characterization and use of collagen I hydrogels for research can be most efficiently conducted. This review is divided into three sections: (1) relevant fabrication parameters are introduced and several of the most popular methods of controlling and regulating them are described, (2) hydrogel properties most relevant for tissue engineering are presented and discussed along with their characterization techniques, (3) the state of collagen I hydrogel characterization is recapitulated and future directions are proposed. Ultimately, this review can serve as a resource for selection of fabrication parameters and material characterization methodologies in order to increase the usefulness of future collagenhydrogel-based characterization studies and tissue engineering experiments.

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Section: Introductionmentioning

confidence: 99%

Review of Collagen I Hydrogels for Bioengineered Tissue Microenvironments: Characterization of Mechanics, Structure, and Transport

Antoine

Vlachos

Rylander³

2014

Tissue Engineering Part B: Reviews

477

353

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“…Parameters like medium composition, shear stress, chemical gradients and temperature are of important consideration when designing the system [102, 103]. However, once the appropriate conditions are obtained microfluidic devices provide a tailored, controlled environment for cellular studies.…”

Section: Microfluidic Approaches For Molecular Diagnosis Of Cancermentioning

confidence: 99%

Microfluidics and cancer: are we there yet?

Zhang

Nagrath

2013

Biomed Microdevices

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More than two decades ago, microfluidics began to show its impact in biological research. Since then, the field of microfluidics has evolving rapidly. Cancer is one of the leading causes of death worldwide. Microfluidics holds great promise in cancer diagnosis and also serves as an emerging tool for understanding cancer biology. Microfluidics can be valuable for cancer investigation due to its high sensitivity, high throughput, less material-consumption, low cost, and enhanced spatio-temporal control. The physical laws on microscale offer an advantage enabling the control of physics, biology, chemistry and physiology at cellular level. Furthermore, microfluidic based platforms are portable and can be easily designed for point-of-care diagnostics. Developing and applying the state of the art microfluidic technologies to address the unmet challenges in cancer can expand the horizons of not only fundamental biology but also the management of disease and patient care. Despite the various microfluidic technologies available in the field, few have been tested clinically, which can be attributed to the various challenges existing in bridging the gap between the emerging technology and real world applications. We present a review of role of microlfuidcs in cancer research, including the history, recent advances and future directions to explore where the field stand currently in addressing complex clinical challenges and future of it. This review identifies four critical areas in cancer research, in which microfluidics can change the current paradigm. These include cancer cell isolation, molecular diagnostics, tumor biology and high-throughput screening for therapeutics. In addition, some of our lab’s current research is presented in the corresponding sections.

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“…As the physiological relevance of such models with respect to humans increases, the experimental complexity, along with required time, cost, and resources, also increases. Medical and life science researchers have thus adopted models that are as simple, robust, and reproducible as possible but still sufficiently represent the physiological phenomena of interest [5–8]. …”

Section: Introductionmentioning

confidence: 99%

“…However, conventional 2D and 3D static cell culture models often fail to reproduce the critical aspects of human physiology, because cell culture approaches can be difficult to adapt dynamic 3D microenvironments and the simultaneous study of multiple tissues and their interactions [5, 8–10]. For example, 3D cell culture models, in which cells are grown within 3D scaffolds, allow cells to interact with neighboring cells and the extracellular matrix (ECM) [11]; such cell–cell and cell–ECM interactions improve tissue-specific functions.…”

Section: Introductionmentioning

confidence: 99%

Physiologically relevant organs on chips

Yum

Hong

Healy

et al. 2013

Biotechnology Journal

123

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Recent advances in integrating microengineering and tissue engineering have generated promising microengineered physiological models for experimental medicine and pharmaceutical research. Here we review the recent development of microengineered physiological systems, or organs on chips, that reconstitute the physiologically critical features of specific human tissues and organs and their interactions. This technology uses microengineering approaches to construct organ-specific microenvironments, reconstituting tissue structures, tissue–tissue interactions and interfaces, and dynamic mechanical and biochemical stimuli found in specific organs, to direct cells to assemble into functional tissues. We first discuss microengineering approaches to reproduce the key elements of physiologically important, dynamic mechanical microenvironments, biochemical microenvironments, and microarchitectures of specific tissues and organs in microfluidic cell culture systems. This is followed by examples of microengineered individual organ models that incorporate the key elements of physiological microenvironments into single microfluidic cell culture systems to reproduce organ-level functions. Finally, microengineered multiple organ systems that simulate multiple organ interactions to better represent human physiology, including human responses to drugs, is covered in this review. This emerging organs-on-chips technology has the potential to become an alternative to 2D and 3D cell culture and animal models for experimental medicine, human disease modeling, drug development, and toxicology.

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Microtechnology for Mimicking In Vivo Tissue Environment

Cited by 52 publications

References 88 publications

Review of Collagen I Hydrogels for Bioengineered Tissue Microenvironments: Characterization of Mechanics, Structure, and Transport

Review of Collagen I Hydrogels for Bioengineered Tissue Microenvironments: Characterization of Mechanics, Structure, and Transport

Microfluidics and cancer: are we there yet?

Physiologically relevant organs on chips

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