Microtubule-Associated Kinase-like Protein RUNKEL Needed for Cell Plate Expansion in Arabidopsis Cytokinesis

Krupnova, Tamara; Sasabe, Michiko; Ghebreghiorghis, Luam; Gruber, Christian W.; Hamada, Takahiro; Dehmel, Verena; Strompen, Georg; Stierhof, York‐Dieter; Lukowitz, Wolfgang; Kemmerling, Birgit; Machida, Yasunori; Hashimoto, Takashi; Mayer, Ulríke; Jürgens, Gerd

doi:10.1016/j.cub.2009.03.021

Cited by 20 publications

(32 citation statements)

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“…We used a TAN1-YFP fusion containing the CYCLIN1B (GRMZM2G034647) destruction box (D-TAN1-YFP) to assess the function of TAN1 after anaphase. Maize CYCLIN1B is degraded in anaphase (33) and A. thaliana CYCLIN1B homolog has been used to degrade proteins during anaphase (34,35). A negative control with a mutated destruction box was also created (mD-TAN1-YFP).…”

Section: Resultsmentioning

confidence: 99%

Proper division plane orientation and mitotic progression together allow normal growth of maize

Martínez

Luo

Sylvester

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

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How growth, microtubule dynamics, and cell-cycle progression are coordinated is one of the unsolved mysteries of cell biology. A maize mutant, tangled1, with known defects in growth and proper division plane orientation, and a recently characterized cell-cycle delay identified by time-lapse imaging, was used to clarify the relationship between growth, cell cycle, and proper division plane orientation. The tangled1 mutant was fully rescued by introduction of cortical division site localized TANGLED1-YFP. A CYCLIN1B destruction box was fused to TANGLED1-YFP to generate a line that mostly rescued the division plane defect but still showed cell-cycle delays when expressed in the tangled1 mutant. Although an intermediate growth phenotype between wild-type and the tangled1 mutant was expected, these partially rescued plants grew as well as wild-type siblings, indicating that mitotic progression delays alone do not alter overall growth. These data indicate that division plane orientation, together with proper cell-cycle progression, is critical for plant growth.

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Section: Resultsmentioning

confidence: 99%

Proper division plane orientation and mitotic progression together allow normal growth of maize

Martínez

Luo

Sylvester

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

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“…They belong to ULK families already implicated in flagellum-related functions in animals (35,36) with the mammalian ULK Fused demonstrated to be essential for construction of motile cilia (35). Plant orthologs of both kinases were shown to be critical for the phragmoplast organization, resembling the function of plant kinesin-15s (37,38). Moreover, Arabidopsis Fused was shown to interact with the kinesin-15s (39), and the involved motifs are conserved in FCP6/TbFused (SI Appendix, Fig.…”

Section: The Fc Is a Membrane Junction Attached By Two Types Of Kinesmentioning

confidence: 95%

“…S11), indicating that the Fused kinase-kinesin-15 module is evolutionarily ancient. ULK4 from plants is enzymatically inactive and possesses a microtubule-binding activity (37). Such an activity would be advantageous in the context of the FC.…”

Section: The Fc Is a Membrane Junction Attached By Two Types Of Kinesmentioning

confidence: 99%

Protein diversity in discrete structures at the distal tip of the trypanosome flagellum

Varga

Moreira-Leite

Portman

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

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The distal end of the eukaryotic flagellum/cilium is important for axonemal growth and signaling and has distinct biomechanical properties. Specific flagellum tip structures exist, yet their composition, dynamics, and functions are largely unknown. We used biochemical approaches to identify seven constituents of the flagella connector at the tip of an assembling trypanosome flagellum and three constituents of the axonemal capping structure at the tips of both assembling and mature flagella. Both tip structures contain evolutionarily conserved as well as kinetoplastid-specific proteins, and component assembly into the structures occurs very early during flagellum extension. Localization and functional studies reveal that the flagella connector membrane junction is attached to the tips of extending microtubules of the assembling flagellum by a kinesin-15 family member. On the opposite side, a kinetoplastid-specific kinesin facilitates attachment of the junction to the microtubules in the mature flagellum. Functional studies also suggest roles of several other components and the definition of subdomains in the tip structures.

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“…It would be interesting to test whether the function of the cytokinesis specific motor is regulated by TIO. Another kinase-like MAP RUNKEL also decorates the phragmoplast midzone and is required for the organization of the anti-parallel MT array and the expansion of the phragmoplast [53,54]. It will be fascinating to elucidate how so many cytokinesis important factors work together in a spatially and temporally coordinated manner to govern the rise and fall of the phragmoplast MT array.…”

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confidence: 99%

The rise and fall of the phragmoplast microtubule array

Lee

Liu

2013

Current Opinion in Plant Biology

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The cytokinetic apparatus, the phragmoplast, contains a bipolar microtubule (MT) framework that has the MT plus ends concentrated at or near the division site. This anti-parallel MT array provides tracks for the transport of Golgi-derived vesicles toward the plus ends so that materials enclosed are subsequently deposited at the division site. Here we will discuss a proposed model of the centrifugal expansion of the phragmoplast that takes place concomitantly with the assembly of the cell plate, the ultimate product of vesicle fusion. The expansion is a result of continuous MT assembly at the phragmoplast periphery while the MTs toward the center of the phragmoplast are disassembled. These events are the result of MT-dependent MT polymerization, bundling of antiparallel MTs coming from opposite sides of the division plane that occurs selectively at the phragmoplast periphery, positioning of the plus ends of cross-linked MTs at or near the division site by establishing a minimal MT-overlapping zone, and debundling of anti-parallel MTs that is triggered by phosphorylation of MT-associated proteins. The debundled MTs are disassembled at last by factors including the MT severing enzyme katanin. IntroductionThe innovation of the phragmoplast marks a significant advancement in the evolution from green algae to land plants [1]. The phragmoplast contains a core of two mirrored sets of anti-parallel microtubules (MTs) whose plus ends are concentrated at or near the midzone of this cytokinetic apparatus (Figure 1) [2]. The ultimate mission of this bipolar MT array is to allow Golgi-derived vesicles to be transported unidirectionally toward MT plus ends so that the materials enclosed in these vesicles are deposited for the assembly of the cell plate. Phragmoplast MTs, like those MTs in spindles during mitosis, undergo continuous remodeling throughout cytokinesis [3,4]. Upon the completion of anaphase, MTs are polymerized and coalesced in the spindle midzone, and a bipolar array is established as the Kinesin-5 motor acts on anti-parallel MTs to slide them against each other ( Figure 1a) [5,6 ,7]. Concomitant with the addition of more MTs to the array, the MTs are shortened at their minus ends, which are facing the reforming daughter nuclei (Figure 1b). One of the most spectacular phenomena in cytokinesis is the centrifugal expansion of the phragmoplast MT array from the cell center to the edge. During the expansion, new MT filaments are added to the periphery of the phragmoplast while older ones in the inner part of the phragmoplast array are disassembled ( Figure 1c). This is particularly challenging because those opposing activities occur simultaneously within a few microns of each other. The assembly of new MTs uses tubulin subunits released from the depolymerization of older MTs [3].Before MT-associated proteins (MAPs) and MT-based motors were identified, microscopic observations had revealed many structural details of the phragmoplast in elegant systems like the endosperm of the African blood lily Haemanthus and tobacc...

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Microtubule-Associated Kinase-like Protein RUNKEL Needed for Cell Plate Expansion in Arabidopsis Cytokinesis

Cited by 20 publications

References 0 publications

Proper division plane orientation and mitotic progression together allow normal growth of maize

Proper division plane orientation and mitotic progression together allow normal growth of maize

Protein diversity in discrete structures at the distal tip of the trypanosome flagellum

The rise and fall of the phragmoplast microtubule array

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