Microtubule-associated protein IQ67 DOMAIN5 regulates morphogenesis of leaf pavement cells in<i>Arabidopsis thaliana</i>

Mitra, Dipannita; Klemm, Sandra; Kumari, Pratibha; Quegwer, Jakob; Möller, Birgit; Poeschl, Yvonne; Pflug, Paul; Stamm, Gina; Abel, Steffen; Bürstenbinder, Katharina

doi:10.1093/jxb/ery395

Cited by 41 publications

(34 citation statements)

References 93 publications

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“…MT; microtubule; ns, not significant. (Ambrose et al, 2007;Kirik et al, 2007;Lin et al, 2013;Liang et al, 2018;Mitra et al, 2019). Decreased lobe morphogenesis in some mutants may be caused by the change in growth and organ shape from isotropic to elongated growth patterns (Lee et al, 2006;Sapala et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Basic Proline-Rich Protein-Mediated Microtubules Are Essential for Lobe Growth and Flattened Cell Geometry

et al. 2019

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Complex cell shapes are generated first by breaking symmetry, and subsequent polar growth. Localized bending of anticlinal walls initiates lobe formation in the epidermal pavement cells of cotyledons and leaves, but how the microtubule cytoskeleton mediates local cell growth, and how plant pavement cells benefit from adopting jigsaw puzzle-like shapes, are poorly understood. In Arabidopsis (Arabidopsis thaliana), the basic Pro-rich protein (BPP) microtubule-associated protein family comprises seven members. We analyzed lobe morphogenesis in cotyledon pavement cells of a BPP1;BPP2;BPP5 triple knockout mutant. New image analysis methods (MtCurv and BQuant) showed that anticlinal microtubule bundles were significantly reduced and cortical microtubules that fan out radially across the periclinal wall did not enrich at the convex side of developing lobes. Despite these microtubule defects, new lobes were initiated at the same frequency as in wild-type cells, but they did not expand into well-defined protrusions. Eventually, mutant cells formed nearly polygonal shapes and adopted concentric microtubule patterns. The mutant periclinal cell wall bulged outward. The radius of the calculated inscribed circle of the pavement cells, a proposed proxy for maximal stress in the cell wall, was consistently larger in the mutant cells during cotyledon development, and correlated with an increase in cell height. These bpp mutant phenotypes provide genetic and cell biological evidence that initiation and growth of lobes are distinct morphogenetic processes, and that interdigitated cell geometry effectively suppresses large outward bulging of pavement cells.

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Section: Discussionmentioning

confidence: 99%

Basic Proline-Rich Protein-Mediated Microtubules Are Essential for Lobe Growth and Flattened Cell Geometry

et al. 2019

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“…Disordered cortical microtubules usually correlate with wider pavement cell indentations and reduced lobe length. Due to the abnormal expression of IQD5, IQD11, IQD14, IQD16, and IQD25 in Arabidopsis , cortical microtubules become disordered in pavement cells to affect their shape, indicating that IQD proteins may regulate anisotropic growth and shape formation by regulating the order of cortical microtubules ( Burstenbinder et al, 2017b ; Liang et al, 2018 ; Mitra et al, 2019 ). Different IQDs affect microtubule organization in different ways to produce unique phenotypes ( Liang et al, 2018 ).…”

Section: The Role Of Iqd On Organ Shapementioning

confidence: 99%

“…Different IQDs affect microtubule organization in different ways to produce unique phenotypes ( Liang et al, 2018 ). Due to the limitations of intracellular Ca 2+ imaging and the functional redundancy of the IQD family, the specific regulatory mechanisms are still unclear ( Mitra et al, 2019 ). It is complex.…”

Section: The Role Of Iqd On Organ Shapementioning

confidence: 99%

Section: The Role Of Iqd On Organ Shapementioning

confidence: 99%

“…Furthermore, Ca 2+ signaling plays a key role for the pavement cell morphology and IQD5’s recruitment to cortical microtubules ( Mitra et al, 2019 ). The IQD-KLCR module stabilizes cortical microtubules laterally, especially at the microtubule-plasma membrane interface ( Mitra et al, 2019 ). Unlike IQD5, which inhibits microtubule dynamics to stabilize microtubules, microtubule-associated proteins exist in Arabidopsis that affect microtubule organization by promoting their growth, contraction, and catastrophe frequency, thereby enhancing microtubule dynamics and ensuring normal sorting ( Liang et al, 2018 ) [e.g., MOR1 in the Arabidopsis MAP215 family ( Twell et al, 2002 )].…”

Section: The Role Of Iqd On Organ Shapementioning

confidence: 99%

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New Insights Into Functions of IQ67-Domain Proteins

Guo

Zhou

2021

Front. Plant Sci.

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IQ67-domain (IQD) proteins, first identified in Arabidopsis and rice, are plant-specific calmodulin-binding proteins containing highly conserved motifs. They play a critical role in plant defenses, organ development and shape, and drought tolerance. Driven by comprehensive genome identification and analysis efforts, IQDs have now been characterized in several species and have been shown to act as microtubule-associated proteins, participating in microtubule-related signaling pathways. However, the precise molecular mechanisms underpinning their biological functions remain incompletely understood. Here we review current knowledge on how IQD family members are thought to regulate plant growth and development by affecting microtubule dynamics or participating in microtubule-related signaling pathways in different plant species and propose some new insights.

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Investigation of Glycosylphosphatidylinositol (GPI)‐Plasma Membrane Interaction in Live Cells and the Influence of GPI Glycan Structure on the Interaction

Kundu,

Jaiswal,

Babu Mullapudi

et al. 2023

Chemistry A European J

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Glycosylphosphatidylinositols (GPIs) need to interact with other components in the cell membrane to transduce transmembrane signals. A bifunctional GPI probe was employed for photoaffinity‐based proximity labelling and identification of GPI‐interacting proteins in the cell membrane. This probe contained the entire core structure of GPIs and was functionalized with photoreactive diazirine and clickable alkyne to facilitate its crosslinking with proteins and attachment of an affinity tag. It was disclosed that this probe was more selective than our previously reported probe containing only a part structure of the GPI core for cell membrane incorporation and an improved probe for studying GPI‐cell membrane interaction. Eighty‐eight unique membrane proteins, many of which are related to GPIs/GPI‐anchored proteins, were identified utilizing this probe. The proteomics dataset is a valuable resource for further analyses and data mining to find new GPI‐related proteins and signalling pathways. A comparison of these results with those of our previous probe provided direct evidence for the profound impact of GPI glycan structure on its interaction with the cell membrane.

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Microtubule-associated protein IQ67 DOMAIN5 regulates morphogenesis of leaf pavement cells inArabidopsis thaliana

Abstract: Microtubule-localized Arabidopsis IQ67 DOMAIN5 regulates pavement cell morphogenesis in the leaf epidermis and links calcium–calmodulin signaling to anisotropic expansion during early phases of pavement cell growth.

Cited by 41 publications

References 93 publications

Basic Proline-Rich Protein-Mediated Microtubules Are Essential for Lobe Growth and Flattened Cell Geometry

Basic Proline-Rich Protein-Mediated Microtubules Are Essential for Lobe Growth and Flattened Cell Geometry

New Insights Into Functions of IQ67-Domain Proteins

Investigation of Glycosylphosphatidylinositol (GPI)‐Plasma Membrane Interaction in Live Cells and the Influence of GPI Glycan Structure on the Interaction

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