Microtubule bundling plays a role in ethylene-mediated cortical microtubule reorientation in etiolated <i>Arabidopsis</i> hypocotyls

Ma, Qin; Sun, Jingbo; Mao, Tonglin

doi:10.1242/jcs.184408

Cited by 28 publications

(25 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…An additional method 196 was used for quantification of the total microtubule length per unit surface area (Fujita 197 et al, 2013 salt stress (Asensi-Fabado et al, 2012;Li et al, 2016). The mutant ein3eil1 is 208 hypersensitive to NaCl treatment (Peng et al, 2014 signaling-inhibited hypocotyl cell elongation in darkness (Sun et al, 2015;Ma et al, 256 2016 Similar to other reports, we found that activation of ethylene signaling greatly alters 374 organization and stability of cortical microtubules in hypocotyl cells (Sun et al, 2015; 375 Ma et al, 2016Ma et al, , 2018. Other phytohormones, such as auxin and brassinosteroid (BR), 376 also affect cortical microtubule organization and stability (Wang et al, 2012;Vineyard 377 et al, 2013).…”

supporting

confidence: 68%

“…The results of several studies have demonstrated that ethylene regulates the stability 138 and organization of cortical microtubules in cells from roots and etiolated hypocotyls 139 Verbelen et al, 2008;Sun et al, 2015;Ma et al, 2016). For example, (WDL5) participates in ethylene signaling to inhibit etiolated hypocotyl elongation 142 (Sun et al, 2015 indicated that bleaching is associated with cell death (Supplemental Fig.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Ethylene Signaling Modulates Cortical Microtubule Reassembly in Response to Salt Stress

Dou

Higaki

et al. 2018

Plant Physiol.

Self Cite

View full text Add to dashboard Cite

show abstract

supporting

confidence: 68%

mentioning

confidence: 99%

Ethylene Signaling Modulates Cortical Microtubule Reassembly in Response to Salt Stress

Dou

Higaki

et al. 2018

Plant Physiol.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Ethylene is demonstrated to participate in the cortical microtubule reorganization during etiolated hypocotyl cell elongation (Sun et al, 2015;Ma et al, 2016). Because MDP60 plays a positive role in ethylene-promoted hypocotyl elongation, we analyzed the effects of MDP60 in regulating cortical microtubule organization in response to ethylene.…”

Section: Cortical Microtubule Organization Is Obviously Altered In Epmentioning

confidence: 99%

Coordinated Regulation of Hypocotyl Cell Elongation by Light and Ethylene through a Microtubule Destabilizing Protein

Wang

Sun

et al. 2017

Plant Physiol.

Self Cite

View full text Add to dashboard Cite

Precise regulation of hypocotyl cell elongation is essential for plant growth and survival. Light suppresses hypocotyl elongation by degrading transcription factor phytochrome-interacting factor 3 (PIF3), whereas the phytohormone ethylene promotes hypocotyl elongation by activating PIF3. However, the underlying mechanisms regarding how these two pathways coordinate downstream effectors to mediate hypocotyl elongation are largely unclear. In this study, we identified the novel MicrotubuleDestabilizing Protein 60 (MDP60), which plays a positive role in hypocotyl cell elongation in Arabidopsis (Arabidopsis thaliana); this effect is mediated through PIF3. Ethylene signaling up-regulates MDP60 expression via PIF3 binding to the MDP60 promoter. MDP60 loss-of-function mutants exhibit much shorter hypocotyls, whereas MDP60 overexpression significantly promotes hypocotyl cell elongation when grown in light compared to the control. MDP60 protein binds to microtubules in vitro and in vivo. The organization of cortical microtubules was significantly disrupted in mdp60 mutant cells and MDP60-overexpressing seedlings. These findings indicate that MDP60 is an important mediator of hypocotyl cell elongation. This study reveals a mechanism in which light and ethylene signaling coordinate MDP60 expression to modulate hypocotyl cell elongation by altering cortical microtubules in Arabidopsis.

show abstract

“…A recent study shows that ethylene signaling participates in regulation of cortical microtubule reorientation and microtubule bundling plays important role in this process. The effects of ethylene on microtubule bundling are partially suppressed in a microtubule-bundling protein WDL5 knockout mutant (wdl5-1) [21]. Another study demonstrates that WDL5 plays a positive role in the phytohormones ethylene negatively regulated etiolated hypocotyls cell elongation.…”

mentioning

confidence: 95%

“…The etiolated hypocotyls of aug8 are significantly shorter than WT. AUG8 binds to the plus ends of growing cortical MTs and promotes microtubule reorientation by regulating the dynamic instability of microtubule plus ends [17].WDL3 and WDL5 belong to the microtubule-associated protein Wave-Dampened2 (WVD2)/WVD2-LIKE (WDL) family in Arabidopsis [18][19][20][21]. WDL3 involves in light inhibited Hypocotyl elongation through the degraded by the ubiquitin-26S proteasomedependent pathway in the dark [18].…”

mentioning

confidence: 99%

The Role of Microtubule Associated Proteins in Plant Hypocotyl Elongation

Che¹

2018

BJSTR

View full text Add to dashboard Cite

IntroductionHypocotyl is the part of a plant embryo or seedling plant that is between the cotyledons and the radicle or root. The regulation of hypocotyls elongation is crucial in the process of plant growth and development, because after the seeds germination, hypocotyls fully elongate to search of the soil surface and percept light then significantly inhibits hypocotyls elongation, the cotyledons unfold and the photosynthetic growth process could successfully begin. The Arabidopsis hypocotyls is widely used as a model for studies of cell elongation as hypocotyls cells elongate quickly without division during the postembryonic growth process [1,2]. Hypocotyl cells growth in the darkness start their elongation at the base and proceed in the acropetal direction reach their maximum length. The elongation of hypocotyls cells is regulated by both external and internal cues such as light, phytohormones, transcription factors, and the cytoskeleton [3][4][5].Microtubule (MT) is one of the main members of cytoskeleton and plays important role in regulating cell expansion, division, and plant cell morphogenesis. Cortical microtubules regulate cell elongation by orientating cellulose fibrils and cellulose fibril arrays, thereby influencing the mechanical properties of the cell wall [6][7][8][9]. The orientations of cortical microtubules are associated with the growth status of etiolated hypocotyls [10,11]. The parallel array of cortical microtubules is dominantly transversely oriented to the hypocotyls longitudinal growth axis in elongating hypocotyls cells and longitudinally oriented when cell elongation stops [10], suggesting the regulation of the organization and dynamics of cortical microtubules is crucial for hypocotyls cell growth. The organization and dynamics of cortical microtubules is regulated by microtubule associated proteins (MAPs) [12]. In this article, plants MAPs involve in hypocotyls elongation are classified, on the basis of their function, into two categories: one is positive regulator and the other is negative regulator in the elongation of hypocotyls. In addition, we also discuss how these proteins mediate the synergistic and antagonistic effects in hypocotyls elongation. MAPs Play Positive Roles In Hypocotyl ElongationUntil now, several MAPs play positive roles in regulation of Hypocotyl elongation have been identified. Arabidopsis SPIRAL1 (SPR1) encodes a plant-specific MT-localizing protein [13]. Plants over expressing SPR1 shows enhanced resistance to an MTdisrupting drug and increased Hypocotyl elongation. In addition, the site of high SPR1 expression moved up wards along darkgrown hypocotyls. The temporal and spatial distribution of SPR1-expressing cells in hypocotyls generally matches the zone of rapid cell elongation [13,14]. Moreover, microtubule destabilizing protein 40 (MDP40) as a positive regulator involves in Brassinosteroid (BR) mediated Hypocotyl elongation by destabilizing cortical microtubules [15]. MDP40 is the target of Brassinazole-resistant1 (BZR1), a key transcription factor in...

show abstract

Microtubule bundling plays a role in ethylene-mediated cortical microtubule reorientation in etiolated Arabidopsis hypocotyls

Cited by 28 publications

References 34 publications

Ethylene Signaling Modulates Cortical Microtubule Reassembly in Response to Salt Stress

Ethylene Signaling Modulates Cortical Microtubule Reassembly in Response to Salt Stress

Coordinated Regulation of Hypocotyl Cell Elongation by Light and Ethylene through a Microtubule Destabilizing Protein

The Role of Microtubule Associated Proteins in Plant Hypocotyl Elongation

Contact Info

Product

Resources

About