Microtubules

Fedtke, Carl

doi:10.1007/978-3-642-68375-6_6

Cited by 1 publication

(2 citation statements)

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“…Most known and suspected antimicrotubule herbicides have relatively low aqueous solubilities and may bind to the surfaces of containers and be lost from solution (Fedtke, 1982;Hugdahl and Morejohn, 1993). Pilot experiments were carried out to determine the solubility characteristics of APM in IB solutions containing 2% (v/v) DMSO.…”

Section: Quasi-equilibrium Dialysis Measurementsmentioning

confidence: 99%

“…Because APM has a relatively low solubility ir( aqueous solutions (Fedtke, 1982) and may bind to the dialysi:; chamber as does oryzalin , pilot studies were conducted on its solubility in the buffer used for ligandbinding experiments. It was determined that an APM concentration 5110 p~ remains constant for periods up to 3 h, as described in "Materials and Methods.…”

Section: Competition Of Apm With Oryzalin For Binding To Tobacco Kubulinmentioning

confidence: 99%

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Competitive Inhibition of High-Affinity Oryzalin Binding to Plant Tubulin by the Phosphoric Amide Herbicide Amiprophos-Methyl

et al. 1994

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Amiprophos-methyl (APM), a phosphoric amide herbicide, was previously reported to inhibit the in vitro polymerization of isolated plant tubulin (L.C. Morejohn, D.E. Fosket [1984] Science 224: 874-876), yet little other biochemical information exists concerning this compound. To characterize further the mechanism of action of APM, its interactions with tubulin and microtubules purified from cultured cells of tobacco (Nicofiana tabacum cv Bright Yellow-2) were investigated. Low micromolar concentrations of APM depolymerized preformed, taxol-stabilized tobacco microtubules. Remarkably, at the lowest APM concentration examined, many short microtubules were redistributed into fewer but 2.7-fold longer microtubules without a substantial decrease in total polymer mass, a result consistent with an end-to-end annealing of microtubules with enhanced kinetic properties. Quasi-equilibrium binding measurements showed that tobacco tubulin binds ['4C]oryzalin with high affinity to produce a tubulin-oryzalin complex having a dis- Phosphoric amide herbicides such as APM (Tokunol M) and butamiphos (Cremart) were developed as preemergence herbicides and are effective on annual grasses and broadleaf weeds (Aya et al., 1975). The uses of phosphoric amides are similar to those of the dinitroaniline herbicides, including

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Section: Quasi-equilibrium Dialysis Measurementsmentioning

confidence: 99%

Section: Competition Of Apm With Oryzalin For Binding To Tobacco Kubulinmentioning

confidence: 99%